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Single-cell RNA-sequencing analysis of the developing mouse inner ear identifies molecular logic of auditory neuron diversification

Author

Listed:
  • Charles Petitpré

    (Karolinska Institutet)

  • Louis Faure

    (Medical University Vienna)

  • Phoebe Uhl

    (Karolinska Institutet)

  • Paula Fontanet

    (Karolinska Institutet)

  • Iva Filova

    (Institute of Biotechnology CAS)

  • Gabriela Pavlinkova

    (Institute of Biotechnology CAS)

  • Igor Adameyko

    (Medical University Vienna
    Karolinska Institutet)

  • Saida Hadjab

    (Karolinska Institutet)

  • Francois Lallemend

    (Karolinska Institutet
    Ming-Wai Lau Centre for Reparative Medicine, Stockholm Node, Karolinska Institutet)

Abstract

Different types of spiral ganglion neurons (SGNs) are essential for auditory perception by transmitting complex auditory information from hair cells (HCs) to the brain. Here, we use deep, single cell transcriptomics to study the molecular mechanisms that govern their identity and organization in mice. We identify a core set of temporally patterned genes and gene regulatory networks that may contribute to the diversification of SGNs through sequential binary decisions and demonstrate a role for NEUROD1 in driving specification of a Ic-SGN phenotype. We also find that each trajectory of the decision tree is defined by initial co-expression of alternative subtype molecular controls followed by gradual shifts toward cell fate resolution. Finally, analysis of both developing SGN and HC types reveals cell-cell signaling potentially playing a role in the differentiation of SGNs. Our results indicate that SGN identities are drafted prior to birth and reveal molecular principles that shape their differentiation and will facilitate studies of their development, physiology, and dysfunction.

Suggested Citation

  • Charles Petitpré & Louis Faure & Phoebe Uhl & Paula Fontanet & Iva Filova & Gabriela Pavlinkova & Igor Adameyko & Saida Hadjab & Francois Lallemend, 2022. "Single-cell RNA-sequencing analysis of the developing mouse inner ear identifies molecular logic of auditory neuron diversification," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31580-1
    DOI: 10.1038/s41467-022-31580-1
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