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Characterization of the development of the mouse cochlear epithelium at the single cell level

Author

Listed:
  • Likhitha Kolla

    (National Institutes of Health)

  • Michael C. Kelly

    (National Institutes of Health)

  • Zoe F. Mann

    (King’s College London)

  • Alejandro Anaya-Rocha

    (National Institutes of Health)

  • Kathryn Ellis

    (National Institutes of Health)

  • Abigail Lemons

    (National Institutes of Health)

  • Adam T. Palermo

    (Decibel Therapeutics)

  • Kathy S. So

    (Decibel Therapeutics)

  • Joseph C. Mays

    (National Institutes of Health)

  • Joshua Orvis

    (University of Maryland School of Medicine)

  • Joseph C. Burns

    (Decibel Therapeutics)

  • Ronna Hertzano

    (University of Maryland School of Medicine
    University of Maryland School of Medicine)

  • Elizabeth C. Driver

    (National Institutes of Health)

  • Matthew W. Kelley

    (National Institutes of Health)

Abstract

Mammalian hearing requires the development of the organ of Corti, a sensory epithelium comprising unique cell types. The limited number of each of these cell types, combined with their close proximity, has prevented characterization of individual cell types and/or their developmental progression. To examine cochlear development more closely, we transcriptionally profile approximately 30,000 isolated mouse cochlear cells collected at four developmental time points. Here we report on the analysis of those cells including the identification of both known and unknown cell types. Trajectory analysis for OHCs indicates four phases of gene expression while fate mapping of progenitor cells suggests that OHCs and their surrounding supporting cells arise from a distinct (lateral) progenitor pool. Tgfβr1 is identified as being expressed in lateral progenitor cells and a Tgfβr1 antagonist inhibits OHC development. These results provide insights regarding cochlear development and demonstrate the potential value and application of this data set.

Suggested Citation

  • Likhitha Kolla & Michael C. Kelly & Zoe F. Mann & Alejandro Anaya-Rocha & Kathryn Ellis & Abigail Lemons & Adam T. Palermo & Kathy S. So & Joseph C. Mays & Joshua Orvis & Joseph C. Burns & Ronna Hertz, 2020. "Characterization of the development of the mouse cochlear epithelium at the single cell level," Nature Communications, Nature, vol. 11(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16113-y
    DOI: 10.1038/s41467-020-16113-y
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    Cited by:

    1. Charles Petitpré & Louis Faure & Phoebe Uhl & Paula Fontanet & Iva Filova & Gabriela Pavlinkova & Igor Adameyko & Saida Hadjab & Francois Lallemend, 2022. "Single-cell RNA-sequencing analysis of the developing mouse inner ear identifies molecular logic of auditory neuron diversification," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Marina Kaiser & Timo H. Lüdtke & Lena Deuper & Carsten Rudat & Vincent M. Christoffels & Andreas Kispert & Mark-Oliver Trowe, 2022. "TBX2 specifies and maintains inner hair and supporting cell fate in the Organ of Corti," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    3. Huihui Liu & Hongchao Liu & Longhao Wang & Lei Song & Guixian Jiang & Qing Lu & Tao Yang & Hu Peng & Ruijie Cai & Xingle Zhao & Ting Zhao & Hao Wu, 2023. "Cochlear transcript diversity and its role in auditory functions implied by an otoferlin short isoform," Nature Communications, Nature, vol. 14(1), pages 1-19, December.

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