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Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates

Author

Listed:
  • Louis Faure

    (Medical University Vienna)

  • Yiqiao Wang

    (Karolinska Institutet)

  • Maria Eleni Kastriti

    (Medical University Vienna)

  • Paula Fontanet

    (Karolinska Institutet)

  • Kylie K. Y. Cheung

    (Karolinska Institutet)

  • Charles Petitpré

    (Karolinska Institutet)

  • Haohao Wu

    (Karolinska Institutet)

  • Lynn Linyu Sun

    (Karolinska Institutet)

  • Karen Runge

    (Aix-Marseille University)

  • Laura Croci

    (Università Vita-Salute San Raffaele)

  • Mark A. Landy

    (UT Southwestern Medical Center)

  • Helen C. Lai

    (UT Southwestern Medical Center)

  • Gian Giacomo Consalez

    (Università Vita-Salute San Raffaele)

  • Antoine Chevigny

    (Aix-Marseille University)

  • François Lallemend

    (Karolinska Institutet
    Karolinska Institutet)

  • Igor Adameyko

    (Medical University Vienna
    Karolinska Institutet)

  • Saida Hadjab

    (Karolinska Institutet)

Abstract

Somatic sensation is defined by the existence of a diversity of primary sensory neurons with unique biological features and response profiles to external and internal stimuli. However, there is no coherent picture about how this diversity of cell states is transcriptionally generated. Here, we use deep single cell analysis to resolve fate splits and molecular biasing processes during sensory neurogenesis in mice. Our results identify a complex series of successive and specific transcriptional changes in post-mitotic neurons that delineate hierarchical regulatory states leading to the generation of the main sensory neuron classes. In addition, our analysis identifies previously undetected early gene modules expressed long before fate determination although being clearly associated with defined sensory subtypes. Overall, the early diversity of sensory neurons is generated through successive bi-potential intermediates in which synchronization of relevant gene modules and concurrent repression of competing fate programs precede cell fate stabilization and final commitment.

Suggested Citation

  • Louis Faure & Yiqiao Wang & Maria Eleni Kastriti & Paula Fontanet & Kylie K. Y. Cheung & Charles Petitpré & Haohao Wu & Lynn Linyu Sun & Karen Runge & Laura Croci & Mark A. Landy & Helen C. Lai & Gian, 2020. "Single cell RNA sequencing identifies early diversity of sensory neurons forming via bi-potential intermediates," Nature Communications, Nature, vol. 11(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-17929-4
    DOI: 10.1038/s41467-020-17929-4
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    Cited by:

    1. Ariel Madrigal & Tianyuan Lu & Larisa M. Soto & Hamed S. Najafabadi, 2024. "A unified model for interpretable latent embedding of multi-sample, multi-condition single-cell data," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    2. Benjamin Villalard & Arjan Boltjes & Florie Reynaud & Olivier Imbaud & Karine Thoinet & Ilse Timmerman & Séverine Croze & Emy Theoulle & Gianluigi Atzeni & Joël Lachuer & Jan J. Molenaar & Godelieve A, 2024. "Neuroblastoma plasticity during metastatic progression stems from the dynamics of an early sympathetic transcriptomic trajectory," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    3. Charles Petitpré & Louis Faure & Phoebe Uhl & Paula Fontanet & Iva Filova & Gabriela Pavlinkova & Igor Adameyko & Saida Hadjab & Francois Lallemend, 2022. "Single-cell RNA-sequencing analysis of the developing mouse inner ear identifies molecular logic of auditory neuron diversification," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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