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Stereocilia-staircase spacing is influenced by myosin III motors and their cargos espin-1 and espin-like

Author

Listed:
  • Seham Ebrahim

    (Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health)

  • Matthew R. Avenarius

    (Oregon Hearing Research Center and Vollum Institute, Oregon Health & Science University)

  • M’hamed Grati

    (Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health)

  • Jocelyn F. Krey

    (Oregon Hearing Research Center and Vollum Institute, Oregon Health & Science University)

  • Alanna M. Windsor

    (Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health)

  • Aurea D. Sousa

    (Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health)

  • Angela Ballesteros

    (Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health)

  • Runjia Cui

    (Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health)

  • Bryan A. Millis

    (Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health)

  • Felipe T. Salles

    (Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health)

  • Michelle A. Baird

    (Florida State University)

  • Michael W. Davidson

    (Florida State University)

  • Sherri M. Jones

    (University of Nebraska-Lincoln)

  • Dongseok Choi

    (Oregon Health and Science University)

  • Lijin Dong

    (Genetic Engineering Core, National Eye Institute, National Institutes of Health)

  • Manmeet H. Raval

    (Penn State University College of Medicine)

  • Christopher M. Yengo

    (Penn State University College of Medicine)

  • Peter G. Barr-Gillespie

    (Oregon Hearing Research Center and Vollum Institute, Oregon Health & Science University)

  • Bechara Kachar

    (Laboratory of Cell Structure and Dynamics, National Institute on Deafness and Other Communication Disorders, National Institutes of Health)

Abstract

Hair cells tightly control the dimensions of their stereocilia, which are actin-rich protrusions with graded heights that mediate mechanotransduction in the inner ear. Two members of the myosin-III family, MYO3A and MYO3B, are thought to regulate stereocilia length by transporting cargos that control actin polymerization at stereocilia tips. We show that eliminating espin-1 (ESPN-1), an isoform of ESPN and a myosin-III cargo, dramatically alters the slope of the stereocilia staircase in a subset of hair cells. Furthermore, we show that espin-like (ESPNL), primarily present in developing stereocilia, is also a myosin-III cargo and is essential for normal hearing. ESPN-1 and ESPNL each bind MYO3A and MYO3B, but differentially influence how the two motors function. Consequently, functional properties of different motor-cargo combinations differentially affect molecular transport and the length of actin protrusions. This mechanism is used by hair cells to establish the required range of stereocilia lengths within a single cell.

Suggested Citation

  • Seham Ebrahim & Matthew R. Avenarius & M’hamed Grati & Jocelyn F. Krey & Alanna M. Windsor & Aurea D. Sousa & Angela Ballesteros & Runjia Cui & Bryan A. Millis & Felipe T. Salles & Michelle A. Baird &, 2016. "Stereocilia-staircase spacing is influenced by myosin III motors and their cargos espin-1 and espin-like," Nature Communications, Nature, vol. 7(1), pages 1-17, April.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms10833
    DOI: 10.1038/ncomms10833
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    Cited by:

    1. Charles Petitpré & Louis Faure & Phoebe Uhl & Paula Fontanet & Iva Filova & Gabriela Pavlinkova & Igor Adameyko & Saida Hadjab & Francois Lallemend, 2022. "Single-cell RNA-sequencing analysis of the developing mouse inner ear identifies molecular logic of auditory neuron diversification," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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