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Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation

Author

Listed:
  • Vidhyavathi Raman

    (Noble Research Institute, LLC
    University of Minnesota)

  • Clemencia M. Rojas

    (Noble Research Institute, LLC
    University of Arkansas)

  • Balaji Vasudevan

    (Noble Research Institute, LLC)

  • Kevin Dunning

    (Noble Research Institute, LLC)

  • Jaydeep Kolape

    (Noble Research Institute, LLC)

  • Sunhee Oh

    (Noble Research Institute, LLC)

  • Jianfei Yun

    (Noble Research Institute, LLC)

  • Lishan Yang

    (Noble Research Institute, LLC)

  • Guangming Li

    (Noble Research Institute, LLC)

  • Bikram D. Pant

    (Noble Research Institute, LLC
    Oklahoma State University
    Oklahoma State University)

  • Qingzhen Jiang

    (Noble Research Institute, LLC)

  • Kirankumar S. Mysore

    (Noble Research Institute, LLC
    Oklahoma State University
    Oklahoma State University)

Abstract

Agrobacterium-mediated plant transformation (AMT) is the basis of modern-day plant biotechnology. One major drawback of this technology is the recalcitrance of many plant species/varieties to Agrobacterium infection, most likely caused by elicitation of plant defense responses. Here, we develop a strategy to increase AMT by engineering Agrobacterium tumefaciens to express a type III secretion system (T3SS) from Pseudomonas syringae and individually deliver the P. syringae effectors AvrPto, AvrPtoB, or HopAO1 to suppress host defense responses. Using the engineered Agrobacterium, we demonstrate increase in AMT of wheat, alfalfa and switchgrass by ~250%–400%. We also show that engineered A. tumefaciens expressing a T3SS can deliver a plant protein, histone H2A-1, to enhance AMT. This strategy is of great significance to both basic research and agricultural biotechnology for transient and stable transformation of recalcitrant plant species/varieties and to deliver proteins into plant cells in a non-transgenic manner.

Suggested Citation

  • Vidhyavathi Raman & Clemencia M. Rojas & Balaji Vasudevan & Kevin Dunning & Jaydeep Kolape & Sunhee Oh & Jianfei Yun & Lishan Yang & Guangming Li & Bikram D. Pant & Qingzhen Jiang & Kirankumar S. Myso, 2022. "Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30180-3
    DOI: 10.1038/s41467-022-30180-3
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    References listed on IDEAS

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    1. Tsuneaki Asai & Guillaume Tena & Joulia Plotnikova & Matthew R. Willmann & Wan-Ling Chiu & Lourdes Gomez-Gomez & Thomas Boller & Frederick M. Ausubel & Jen Sheen, 2002. "MAP kinase signalling cascade in Arabidopsis innate immunity," Nature, Nature, vol. 415(6875), pages 977-983, February.
    2. Florian Lindner & Bailey Milne-Davies & Katja Langenfeld & Thorsten Stiewe & Andreas Diepold, 2020. "LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    3. Yukihiro Akeda & Jorge E. Galán, 2005. "Chaperone release and unfolding of substrates in type III secretion," Nature, Nature, vol. 437(7060), pages 911-915, October.
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