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LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution

Author

Listed:
  • Florian Lindner

    (Max Planck Institute for Terrestrial Microbiology)

  • Bailey Milne-Davies

    (Max Planck Institute for Terrestrial Microbiology)

  • Katja Langenfeld

    (Max Planck Institute for Terrestrial Microbiology)

  • Thorsten Stiewe

    (Philipps-University)

  • Andreas Diepold

    (Max Planck Institute for Terrestrial Microbiology)

Abstract

Many bacteria employ a type III secretion system (T3SS) injectisome to translocate proteins into eukaryotic host cells. Although the T3SS can efficiently export heterologous cargo proteins, a lack of target cell specificity currently limits its application in biotechnology and healthcare. In this study, we exploit the dynamic nature of the T3SS to govern its activity. Using optogenetic interaction switches to control the availability of the dynamic cytosolic T3SS component SctQ, T3SS-dependent effector secretion can be regulated by light. The resulting system, LITESEC-T3SS (Light-induced translocation of effectors through sequestration of endogenous components of the T3SS), allows rapid, specific, and reversible activation or deactivation of the T3SS upon illumination. We demonstrate the light-regulated translocation of heterologous reporter proteins, and induction of apoptosis in cultured eukaryotic cells. LITESEC-T3SS constitutes a new method to control protein secretion and translocation into eukaryotic host cells with unparalleled spatial and temporal resolution.

Suggested Citation

  • Florian Lindner & Bailey Milne-Davies & Katja Langenfeld & Thorsten Stiewe & Andreas Diepold, 2020. "LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-16169-w
    DOI: 10.1038/s41467-020-16169-w
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    Cited by:

    1. Vidhyavathi Raman & Clemencia M. Rojas & Balaji Vasudevan & Kevin Dunning & Jaydeep Kolape & Sunhee Oh & Jianfei Yun & Lishan Yang & Guangming Li & Bikram D. Pant & Qingzhen Jiang & Kirankumar S. Myso, 2022. "Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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