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Molecular Models for the Core Components of the Flagellar Type-III Secretion Complex

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  • William R Taylor
  • Teige R S Matthews-Palmer
  • Morgan Beeby

Abstract

We show that by using a combination of computational methods, consistent three-dimensional molecular models can be proposed for the core proteins of the type-III secretion system. We employed a variety of approaches to reconcile disparate, and sometimes inconsistent, data sources into a coherent picture that for most of the proteins indicated a unique solution to the constraints. The range of difficulty spanned from the trivial (FliQ) to the difficult (FlhA and FliP). The uncertainties encountered with FlhA were largely the result of the greater number of helix packing possibilities allowed in a large protein, however, for FliP, there remains an uncertainty in how to reconcile the large displacement predicted between its two main helical hairpins and their ability to sit together happily across the bacterial membrane. As there is still no high resolution structural information on any of these proteins, we hope our predicted models may be of some use in aiding the interpretation of electron microscope images and in rationalising mutation data and experiments.

Suggested Citation

  • William R Taylor & Teige R S Matthews-Palmer & Morgan Beeby, 2016. "Molecular Models for the Core Components of the Flagellar Type-III Secretion Complex," PLOS ONE, Public Library of Science, vol. 11(11), pages 1-33, November.
  • Handle: RePEc:plo:pone00:0164047
    DOI: 10.1371/journal.pone.0164047
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    References listed on IDEAS

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    1. Koushik Paul & Marc Erhardt & Takanori Hirano & David F. Blair & Kelly T. Hughes, 2008. "Energy source of flagellar type III secretion," Nature, Nature, vol. 451(7177), pages 489-492, January.
    2. Koji Yonekura & Saori Maki-Yonekura & Keiichi Namba, 2003. "Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy," Nature, Nature, vol. 424(6949), pages 643-650, August.
    3. Lawrence K. Lee & Michael A. Ginsburg & Claudia Crovace & Mhairi Donohoe & Daniela Stock, 2010. "Structure of the torque ring of the flagellar motor and the molecular basis for rotational switching," Nature, Nature, vol. 466(7309), pages 996-1000, August.
    4. Yukihiro Akeda & Jorge E. Galán, 2005. "Chaperone release and unfolding of substrates in type III secretion," Nature, Nature, vol. 437(7060), pages 911-915, October.
    5. Raz Zarivach & Wanyin Deng & Marija Vuckovic & Heather B. Felise & Hai V. Nguyen & Samuel I. Miller & B. Brett Finlay & Natalie C. J. Strynadka, 2008. "Structural analysis of the essential self-cleaving type III secretion proteins EscU and SpaS," Nature, Nature, vol. 453(7191), pages 124-127, May.
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