IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-49504-6.html
   My bibliography  Save this article

Natural variation in BnaA07.MKK9 confers resistance to Sclerotinia stem rot in oilseed rape

Author

Listed:
  • Li Lin

    (Yangzhou University
    Yangzhou University)

  • Xingrui Zhang

    (Yangzhou University)

  • Jialin Fan

    (Yangzhou University)

  • Jiawei Li

    (Huazhong Agricultural University)

  • Sichao Ren

    (Yangzhou University)

  • Xin Gu

    (Yangzhou University)

  • Panpan Li

    (Yangzhou University)

  • Meiling Xu

    (Yangzhou University)

  • Jingyi Xu

    (Yangzhou University)

  • Wenjing Lei

    (Yangzhou University)

  • Dongxiao Liu

    (Yangzhou University)

  • Qinfu Sun

    (Yangzhou University)

  • Guangqin Cai

    (Chinese Academy of Agricultural Sciences)

  • Qing-Yong Yang

    (Huazhong Agricultural University)

  • Youping Wang

    (Yangzhou University
    Yangzhou University)

  • Jian Wu

    (Yangzhou University)

Abstract

Sclerotinia stem rot (SSR), caused by the necrotrophic fungus Sclerotinia sclerotiorum, is one of the most devastating diseases for several major oil-producing crops. Despite its impact, the genetic basis of SSR resistance in plants remains poorly understood. Here, through a genome-wide association study, we identify a key gene, BnaA07. MKK9, that encodes a mitogen-activated protein kinase kinase that confers SSR resistance in oilseed rape. Our functional analyses reveal that BnaA07.MKK9 interacts with BnaC03.MPK3 and BnaC03.MPK6 and phosphorylates them at the TEY activation motif, triggering a signaling cascade that initiates biosynthesis of ethylene, camalexin, and indole glucosinolates, and promotes accumulation of H2O2 and the hypersensitive response, ultimately conferring resistance. Furthermore, variations in the coding sequence of BnaA07.MKK9 alter its kinase activity and improve SSR resistance by ~30% in cultivars carrying the advantageous haplotype. These findings enhance our understanding of SSR resistance and may help engineer novel diversity for future breeding of oilseed rape.

Suggested Citation

  • Li Lin & Xingrui Zhang & Jialin Fan & Jiawei Li & Sichao Ren & Xin Gu & Panpan Li & Meiling Xu & Jingyi Xu & Wenjing Lei & Dongxiao Liu & Qinfu Sun & Guangqin Cai & Qing-Yong Yang & Youping Wang & Jia, 2024. "Natural variation in BnaA07.MKK9 confers resistance to Sclerotinia stem rot in oilseed rape," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49504-6
    DOI: 10.1038/s41467-024-49504-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-49504-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-49504-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Shin, Ji-Hyung & Blay, Sigal & McNeney, Brad & Graham, Jinko, 2006. "LDheatmap: An R Function for Graphical Display of Pairwise Linkage Disequilibria Between Single Nucleotide Polymorphisms," Journal of Statistical Software, Foundation for Open Access Statistics, vol. 16(c03).
    2. Jonathan D. G. Jones & Jeffery L. Dangl, 2006. "The plant immune system," Nature, Nature, vol. 444(7117), pages 323-329, November.
    3. Tsuneaki Asai & Guillaume Tena & Joulia Plotnikova & Matthew R. Willmann & Wan-Ling Chiu & Lourdes Gomez-Gomez & Thomas Boller & Frederick M. Ausubel & Jen Sheen, 2002. "MAP kinase signalling cascade in Arabidopsis innate immunity," Nature, Nature, vol. 415(6875), pages 977-983, February.
    4. Kun Lu & Lijuan Wei & Xiaolong Li & Yuntong Wang & Jian Wu & Miao Liu & Chao Zhang & Zhiyou Chen & Zhongchun Xiao & Hongju Jian & Feng Cheng & Kai Zhang & Hai Du & Xinchao Cheng & Cunming Qu & Wei Qia, 2019. "Whole-genome resequencing reveals Brassica napus origin and genetic loci involved in its improvement," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Xiao Zhang & Mark Blaxter & Jonathan M. D. Wood & Alan Tracey & Shane McCarthy & Peter Thorpe & Jack G. Rayner & Shangzhe Zhang & Kirstin L. Sikkink & Susan L. Balenger & Nathan W. Bailey, 2024. "Temporal genomics in Hawaiian crickets reveals compensatory intragenomic coadaptation during adaptive evolution," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    2. Jiaojiao Bai & Yuanyuan Zhou & Jianhang Sun & Kexin Chen & Yufang Han & Ranran Wang & Yanmin Zou & Mingshuo Du & Dongping Lu, 2023. "BIK1 protein homeostasis is maintained by the interplay of different ubiquitin ligases in immune signaling," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    3. Sheng Yang & Weiwei Cai & Ruijie Wu & Yu Huang & Qiaoling Lu & Hui Wang & Xueying Huang & Yapeng Zhang & Qing Wu & Xingge Cheng & Meiyun Wan & Jingang Lv & Qian Liu & Xiang Zheng & Shaoliang Mou & Dey, 2023. "Differential CaKAN3-CaHSF8 associations underlie distinct immune and heat responses under high temperature and high humidity conditions," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    4. Jiahui Liu & Xiaoyun Wu & Yue Fang & Ye Liu & Esther Oreofe Bello & Yong Li & Ruyi Xiong & Yinzi Li & Zheng Qing Fu & Aiming Wang & Xiaofei Cheng, 2023. "A plant RNA virus inhibits NPR1 sumoylation and subverts NPR1-mediated plant immunity," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    5. Paul Vincelli, 2016. "Genetic Engineering and Sustainable Crop Disease Management: Opportunities for Case-by-Case Decision-Making," Sustainability, MDPI, vol. 8(5), pages 1-22, May.
    6. Norliza Abu-Bakar & Nor Mustaiqazah Juri & Ros Azrinawati Hana Abu-Bakar & Mohd Zulfadli Sohaime & Rafidah Badrun & Johari Sarip & Mohd Azhar Hassan & Khairulmazmi Ahmad, 2021. "Recombinant Protein Foliar Application Activates Systemic Acquired Resistance and Increases Tolerance against Papaya Dieback Disease," Asian Journal of Agriculture and rural Development, Asian Economic and Social Society, vol. 11(1), pages 1-9, March.
    7. Xin Tong & Jia-Jia Zhao & Ya-Lan Feng & Jing-Ze Zou & Jian Ye & Junfeng Liu & Chenggui Han & Dawei Li & Xian-Bing Wang, 2023. "A selective autophagy receptor VISP1 induces symptom recovery by targeting viral silencing suppressors," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    8. Huanhuan Li & Wenqiang Men & Chao Ma & Qianwen Liu & Zhenjie Dong & Xiubin Tian & Chaoli Wang & Cheng Liu & Harsimardeep S. Gill & Pengtao Ma & Zhibin Zhang & Bao Liu & Yue Zhao & Sunish K. Sehgal & W, 2024. "Wheat powdery mildew resistance gene Pm13 encodes a mixed lineage kinase domain-like protein," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    9. Farhan Ali & Qingchun Pan & Genshen Chen & Kashif Rafiq Zahid & Jianbing Yan, 2013. "Evidence of Multiple Disease Resistance (MDR) and Implication of Meta-Analysis in Marker Assisted Selection," PLOS ONE, Public Library of Science, vol. 8(7), pages 1-12, July.
    10. Javier Martínez Pacheco & Philippe Ranocha & Luciana Kasulin & Corina M. Fusari & Lucas Servi & Ariel. A. Aptekmann & Victoria Berdion Gabarain & Juan Manuel Peralta & Cecilia Borassi & Eliana Marzol , 2022. "Apoplastic class III peroxidases PRX62 and PRX69 promote Arabidopsis root hair growth at low temperature," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    11. Vidhyavathi Raman & Clemencia M. Rojas & Balaji Vasudevan & Kevin Dunning & Jaydeep Kolape & Sunhee Oh & Jianfei Yun & Lishan Yang & Guangming Li & Bikram D. Pant & Qingzhen Jiang & Kirankumar S. Myso, 2022. "Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    12. Jincai Qiu & Yongshan Chen & Ying Feng & Xiaofeng Li & Jinghua Xu & Jinping Jiang, 2023. "Adaptation of Rhizosphere Microbial Communities to Continuous Exposure to Multiple Residual Antibiotics in Vegetable Farms," IJERPH, MDPI, vol. 20(4), pages 1-15, February.
    13. Hideki Yoshida & Ko Hirano & Kenji Yano & Fanmiao Wang & Masaki Mori & Mayuko Kawamura & Eriko Koketsu & Masako Hattori & Reynante Lacsamana Ordonio & Peng Huang & Eiji Yamamoto & Makoto Matsuoka, 2022. "Genome-wide association study identifies a gene responsible for temperature-dependent rice germination," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    14. Arsheed H. Sheikh & Iosif Zacharia & Alonso J. Pardal & Ana Dominguez-Ferreras & Daniela J. Sueldo & Jung-Gun Kim & Alexi Balmuth & Jose R. Gutierrez & Brendon F. Conlan & Najeeb Ullah & Olivia M. Nip, 2023. "Dynamic changes of the Prf/Pto tomato resistance complex following effector recognition," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    15. Josephine R. Paris & James R. Whiting & Mitchel J. Daniel & Joan Ferrer Obiol & Paul J. Parsons & Mijke J. Zee & Christopher W. Wheat & Kimberly A. Hughes & Bonnie A. Fraser, 2022. "A large and diverse autosomal haplotype is associated with sex-linked colour polymorphism in the guppy," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    16. Shen Huang & Chunli Wang & Zixuan Ding & Yaqian Zhao & Jing Dai & Jia Li & Haining Huang & Tongkai Wang & Min Zhu & Mingfeng Feng & Yinghua Ji & Zhongkai Zhang & Xiaorong Tao, 2024. "A plant NLR receptor employs ABA central regulator PP2C-SnRK2 to activate antiviral immunity," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    17. Karine de Guillen & Diana Ortiz-Vallejo & Jérome Gracy & Elisabeth Fournier & Thomas Kroj & André Padilla, 2015. "Structure Analysis Uncovers a Highly Diverse but Structurally Conserved Effector Family in Phytopathogenic Fungi," PLOS Pathogens, Public Library of Science, vol. 11(10), pages 1-27, October.
    18. Veronika DUMALASOVÁ & Leona SVOBODOVÁ & Alena HANZALOVÁ, 2012. "Differentially expressed gene transcripts in wheat and barley leaves upon leaf spot infection," Czech Journal of Genetics and Plant Breeding, Czech Academy of Agricultural Sciences, vol. 48(3), pages 108-119.
    19. Juan E. Rosas & Maia Escobar & Sebastián Martínez & Pedro Blanco & Fernando Pérez & Gastón Quero & Lucía Gutiérrez & Victoria Bonnecarrère, 2020. "Epistasis and Quantitative Resistance to Pyricularia oryzae Revealed by GWAS in Advanced Rice Breeding Populations," Agriculture, MDPI, vol. 10(12), pages 1-16, December.
    20. Mariam Amouzoune & Sajid Rehman & Rachid Benkirane & Swati Verma & Sanjaya Gyawali & Muamar Al-Jaboobi & Ramesh Pal Singh Verma & Zakaria Kehel & Ahmed Amri, 2022. "Genome-Wide Association Study of Leaf Rust Resistance at Seedling and Adult Plant Stages in a Global Barley Panel," Agriculture, MDPI, vol. 12(11), pages 1-26, November.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49504-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.