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FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteria

Author

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  • Manuel Halte

    (Humboldt-Universität zu Berlin)

  • Ekaterina P. Andrianova

    (The Ohio State University)

  • Christian Goosmann

    (Max Planck Institute for Infection Biology)

  • Fabienne F. V. Chevance

    (University of Utah)

  • Kelly T. Hughes

    (University of Utah)

  • Igor B. Zhulin

    (The Ohio State University)

  • Marc Erhardt

    (Humboldt-Universität zu Berlin
    Max Planck Unit for the Science of Pathogens)

Abstract

The bacterial flagellum, which facilitates motility, is composed of ~20 structural proteins organized into a long extracellular filament connected to a cytoplasmic rotor-stator complex via a periplasmic rod. Flagellum assembly is regulated by multiple checkpoints that ensure an ordered gene expression pattern coupled to the assembly of the various building blocks. Here, we use epifluorescence, super-resolution, and transmission electron microscopy to show that the absence of a periplasmic protein (FlhE) prevents proper flagellar morphogenesis and results in the formation of periplasmic flagella in Salmonella enterica. The periplasmic flagella disrupt cell wall synthesis, leading to a loss of normal cell morphology resulting in cell lysis. We propose that FlhE functions as a periplasmic chaperone to control assembly of the periplasmic rod, thus preventing formation of periplasmic flagella.

Suggested Citation

  • Manuel Halte & Ekaterina P. Andrianova & Christian Goosmann & Fabienne F. V. Chevance & Kelly T. Hughes & Igor B. Zhulin & Marc Erhardt, 2024. "FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteria," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50278-0
    DOI: 10.1038/s41467-024-50278-0
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    References listed on IDEAS

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    1. Koji Yonekura & Saori Maki-Yonekura & Keiichi Namba, 2003. "Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy," Nature, Nature, vol. 424(6949), pages 643-650, August.
    2. Yukihiro Akeda & Jorge E. Galán, 2005. "Chaperone release and unfolding of substrates in type III secretion," Nature, Nature, vol. 437(7060), pages 911-915, October.
    3. M. Leaver & P. Domínguez-Cuevas & J. M. Coxhead & R. A. Daniel & J. Errington, 2009. "Life without a wall or division machine in Bacillus subtilis," Nature, Nature, vol. 457(7231), pages 849-853, February.
    4. M. Leaver & P. Domínguez-Cuevas & J. M. Coxhead & R. A. Daniel & J. Errington, 2009. "Erratum: Life without a wall or division machine in Bacillus subtilis," Nature, Nature, vol. 460(7254), pages 538-538, July.
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