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Pluripotency factors determine gene expression repertoire at zygotic genome activation

Author

Listed:
  • Meijiang Gao

    (University of Freiburg
    Signalling Research Centres BIOSS and CIBSS)

  • Marina Veil

    (University of Freiburg)

  • Marcus Rosenblatt

    (University of Freiburg)

  • Aileen Julia Riesle

    (University of Freiburg)

  • Anna Gebhard

    (University of Freiburg)

  • Helge Hass

    (University of Freiburg)

  • Lenka Buryanova

    (University of Freiburg)

  • Lev Y. Yampolsky

    (East Tennessee State University
    Basel University)

  • Björn Grüning

    (University of Freiburg
    University of Freiburg)

  • Sergey V. Ulianov

    (Russian Academy of Sciences
    M.V. Lomonosov Moscow State University)

  • Jens Timmer

    (Signalling Research Centres BIOSS and CIBSS
    University of Freiburg)

  • Daria Onichtchouk

    (University of Freiburg
    Signalling Research Centres BIOSS and CIBSS
    Koltzov Institute of Developmental Biology RAS)

Abstract

Awakening of zygotic transcription in animal embryos relies on maternal pioneer transcription factors. The interplay of global and specific functions of these proteins remains poorly understood. Here, we analyze chromatin accessibility and time-resolved transcription in single and double mutant zebrafish embryos lacking pluripotency factors Pou5f3 and Sox19b. We show that two factors modify chromatin in a largely independent manner. We distinguish four types of direct enhancers by differential requirements for Pou5f3 or Sox19b. We demonstrate that changes in chromatin accessibility of enhancers underlie the changes in zygotic expression repertoire in the double mutants. Pou5f3 or Sox19b promote chromatin accessibility of enhancers linked to the genes involved in gastrulation and ventral fate specification. The genes regulating mesendodermal and dorsal fates are primed for activation independently of Pou5f3 and Sox19b. Strikingly, simultaneous loss of Pou5f3 and Sox19b leads to premature expression of genes, involved in regulation of organogenesis and differentiation.

Suggested Citation

  • Meijiang Gao & Marina Veil & Marcus Rosenblatt & Aileen Julia Riesle & Anna Gebhard & Helge Hass & Lenka Buryanova & Lev Y. Yampolsky & Björn Grüning & Sergey V. Ulianov & Jens Timmer & Daria Onichtch, 2022. "Pluripotency factors determine gene expression repertoire at zygotic genome activation," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28434-1
    DOI: 10.1038/s41467-022-28434-1
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    References listed on IDEAS

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