IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v503y2013i7476d10.1038_nature12632.html
   My bibliography  Save this article

Nanog, Pou5f1 and SoxB1 activate zygotic gene expression during the maternal-to-zygotic transition

Author

Listed:
  • Miler T. Lee

    (Yale University School of Medicine)

  • Ashley R. Bonneau

    (Yale University School of Medicine)

  • Carter M. Takacs

    (Yale University School of Medicine)

  • Ariel A. Bazzini

    (Yale University School of Medicine)

  • Kate R. DiVito

    (Yale University School of Medicine)

  • Elizabeth S. Fleming

    (Yale University School of Medicine)

  • Antonio J. Giraldez

    (Yale University School of Medicine
    Yale Stem Cell Center, Yale University School of Medicine)

Abstract

After fertilization, maternal factors direct development and trigger zygotic genome activation (ZGA) at the maternal-to-zygotic transition (MZT). In zebrafish, ZGA is required for gastrulation and clearance of maternal messenger RNAs, which is in part regulated by the conserved microRNA miR-430. However, the factors that activate the zygotic program in vertebrates are unknown. Here we show that Nanog, Pou5f1 (also called Oct4) and SoxB1 regulate zygotic gene activation in zebrafish. We identified several hundred genes directly activated by maternal factors, constituting the first wave of zygotic transcription. Ribosome profiling revealed that nanog, sox19b and pou5f1 are the most highly translated transcription factors pre-MZT. Combined loss of these factors resulted in developmental arrest before gastrulation and a failure to activate >75% of zygotic genes, including miR-430. Our results demonstrate that maternal Nanog, Pou5f1 and SoxB1 are required to initiate the zygotic developmental program and induce clearance of the maternal program by activating miR-430 expression.

Suggested Citation

  • Miler T. Lee & Ashley R. Bonneau & Carter M. Takacs & Ariel A. Bazzini & Kate R. DiVito & Elizabeth S. Fleming & Antonio J. Giraldez, 2013. "Nanog, Pou5f1 and SoxB1 activate zygotic gene expression during the maternal-to-zygotic transition," Nature, Nature, vol. 503(7476), pages 360-364, November.
    • Handle: RePEc:nat:nature:v:503:y:2013:i:7476:d:10.1038_nature12632
    DOI: 10.1038/nature12632
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/nature12632
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/nature12632?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Xiaoqing Nie & Qianhua Xu & Chengpeng Xu & Fengling Chen & Qizhi Wang & Dandan Qin & Rui Wang & Zheng Gao & Xukun Lu & Xinai Yang & Yu Wu & Chen Gu & Wei Xie & Lei Li, 2023. "Maternal TDP-43 interacts with RNA Pol II and regulates zygotic genome activation," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Aileen Julia Riesle & Meijiang Gao & Marcus Rosenblatt & Jacques Hermes & Helge Hass & Anna Gebhard & Marina Veil & Björn Grüning & Jens Timmer & Daria Onichtchouk, 2023. "Activator-blocker model of transcriptional regulation by pioneer-like factors," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    3. Meijiang Gao & Marina Veil & Marcus Rosenblatt & Aileen Julia Riesle & Anna Gebhard & Helge Hass & Lenka Buryanova & Lev Y. Yampolsky & Björn Grüning & Sergey V. Ulianov & Jens Timmer & Daria Onichtch, 2022. "Pluripotency factors determine gene expression repertoire at zygotic genome activation," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    4. Lior Fishman & Avani Modak & Gal Nechooshtan & Talya Razin & Florian Erhard & Aviv Regev & Jeffrey A. Farrell & Michal Rabani, 2024. "Cell-type-specific mRNA transcription and degradation kinetics in zebrafish embryogenesis from metabolically labeled single-cell RNA-seq," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    5. Xinru Zhang & Bohao Fang & Yi-Fei Huang, 2023. "Transcription factor binding sites are frequently under accelerated evolution in primates," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    6. Saurabh J. Pradhan & Puli Chandramouli Reddy & Michael Smutny & Ankita Sharma & Keisuke Sako & Meghana S. Oak & Rini Shah & Mrinmoy Pal & Ojas Deshpande & Greg Dsilva & Yin Tang & Rakesh Mishra & Giri, 2021. "Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesis," Nature Communications, Nature, vol. 12(1), pages 1-19, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:503:y:2013:i:7476:d:10.1038_nature12632. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.