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Nanoparticles and photochemistry for native-like transmembrane protein footprinting

Author

Listed:
  • Jie Sun

    (Washington University in St. Louis)

  • Xiaoran Roger Liu

    (Washington University in St. Louis)

  • Shuang Li

    (Washington University School of Medicine)

  • Peng He

    (Washington University School of Medicine)

  • Weikai Li

    (Washington University School of Medicine)

  • Michael L. Gross

    (Washington University in St. Louis)

Abstract

Mass spectrometry-based footprinting can probe higher order structure of soluble proteins in their native states and serve as a complement to high-resolution approaches. Traditional footprinting approaches, however, are hampered for integral membrane proteins because their transmembrane regions are not accessible to solvent, and they contain hydrophobic residues that are generally unreactive with most chemical reagents. To address this limitation, we bond photocatalytic titanium dioxide (TiO2) nanoparticles to a lipid bilayer. Upon laser irradiation, the nanoparticles produce local concentrations of radicals that penetrate the lipid layer, which is made permeable by a simultaneous laser-initiated Paternò–Büchi reaction. This approach achieves footprinting for integral membrane proteins in liposomes, helps locate both ligand-binding residues in a transporter and ligand-induced conformational changes, and reveals structural aspects of proteins at the flexible unbound state. Overall, this approach proves effective in intramembrane footprinting and forges a connection between material science and biology.

Suggested Citation

  • Jie Sun & Xiaoran Roger Liu & Shuang Li & Peng He & Weikai Li & Michael L. Gross, 2021. "Nanoparticles and photochemistry for native-like transmembrane protein footprinting," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
  • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27588-8
    DOI: 10.1038/s41467-021-27588-8
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    1. Dong Deng & Chao Xu & Pengcheng Sun & Jianping Wu & Chuangye Yan & Mingxu Hu & Nieng Yan, 2014. "Crystal structure of the human glucose transporter GLUT1," Nature, Nature, vol. 510(7503), pages 121-125, June.
    2. Sayan Gupta & Jin Chai & Jie Cheng & Rhijuta D’Mello & Mark R. Chance & Dax Fu, 2014. "Visualizing the kinetic power stroke that drives proton-coupled zinc(ii) transport," Nature, Nature, vol. 512(7512), pages 101-104, August.
    3. Dong Deng & Pengcheng Sun & Chuangye Yan & Meng Ke & Xin Jiang & Lei Xiong & Wenlin Ren & Kunio Hirata & Masaki Yamamoto & Shilong Fan & Nieng Yan, 2015. "Molecular basis of ligand recognition and transport by glucose transporters," Nature, Nature, vol. 526(7573), pages 391-396, October.
    4. Anne Kathrine Nielsen & Ingvar R. Möller & Yong Wang & Søren G. F. Rasmussen & Kresten Lindorff-Larsen & Kasper D. Rand & Claus J. Loland, 2019. "Substrate-induced conformational dynamics of the dopamine transporter," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
    5. Weikai Li & Sol Schulman & Rachel J. Dutton & Dana Boyd & Jon Beckwith & Tom A. Rapoport, 2010. "Structure of a bacterial homologue of vitamin K epoxide reductase," Nature, Nature, vol. 463(7280), pages 507-512, January.
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