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How to build a ribosome from RNA fragments in Chlamydomonas mitochondria

Author

Listed:
  • Florent Waltz

    (Université de Bordeaux
    Université de Strasbourg
    Helmholtz Zentrum München)

  • Thalia Salinas-Giegé

    (Université de Strasbourg)

  • Robert Englmeier

    (Utrecht University)

  • Herrade Meichel

    (Université de Strasbourg)

  • Heddy Soufari

    (Université de Bordeaux)

  • Lauriane Kuhn

    (Université de Strasbourg)

  • Stefan Pfeffer

    (Zentrum für Molekulare Biologie der Universität Heidelberg, DKFZ-ZMBH Alliance)

  • Friedrich Förster

    (Utrecht University)

  • Benjamin D. Engel

    (Helmholtz Zentrum München
    Technical University of Munich)

  • Philippe Giegé

    (Université de Strasbourg)

  • Laurence Drouard

    (Université de Strasbourg)

  • Yaser Hashem

    (Université de Bordeaux)

Abstract

Mitochondria are the powerhouse of eukaryotic cells. They possess their own gene expression machineries where highly divergent and specialized ribosomes, named hereafter mitoribosomes, translate the few essential messenger RNAs still encoded by mitochondrial genomes. Here, we present a biochemical and structural characterization of the mitoribosome in the model green alga Chlamydomonas reinhardtii, as well as a functional study of some of its specific components. Single particle cryo-electron microscopy resolves how the Chlamydomonas mitoribosome is assembled from 13 rRNA fragments encoded by separate non-contiguous gene pieces. Additional proteins, mainly OPR, PPR and mTERF helical repeat proteins, are found in Chlamydomonas mitoribosome, revealing the structure of an OPR protein in complex with its RNA binding partner. Targeted amiRNA silencing indicates that these ribosomal proteins are required for mitoribosome integrity. Finally, we use cryo-electron tomography to show that Chlamydomonas mitoribosomes are attached to the inner mitochondrial membrane via two contact points mediated by Chlamydomonas-specific proteins. Our study expands our understanding of mitoribosome diversity and the various strategies these specialized molecular machines adopt for membrane tethering.

Suggested Citation

  • Florent Waltz & Thalia Salinas-Giegé & Robert Englmeier & Herrade Meichel & Heddy Soufari & Lauriane Kuhn & Stefan Pfeffer & Friedrich Förster & Benjamin D. Engel & Philippe Giegé & Laurence Drouard &, 2021. "How to build a ribosome from RNA fragments in Chlamydomonas mitochondria," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27200-z
    DOI: 10.1038/s41467-021-27200-z
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    Cited by:

    1. Haonan Zhang & Yan Li & Yanan Liu & Dongyu Li & Lin Wang & Kai Song & Keyan Bao & Ping Zhu, 2023. "A method for restoring signals and revealing individual macromolecule states in cryo-ET, REST," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. Victor Tobiasson & Ieva Berzina & Alexey Amunts, 2022. "Structure of a mitochondrial ribosome with fragmented rRNA in complex with membrane-targeting elements," Nature Communications, Nature, vol. 13(1), pages 1-9, December.

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