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Organization of the mitochondrial translation machinery studied in situ by cryoelectron tomography

Author

Listed:
  • Stefan Pfeffer

    (Max-Planck Institute of Biochemistry)

  • Michael W. Woellhaf

    (Cell Biology, University of Kaiserslautern)

  • Johannes M. Herrmann

    (Cell Biology, University of Kaiserslautern)

  • Friedrich Förster

    (Max-Planck Institute of Biochemistry)

Abstract

Whereas the structure and function of cytosolic ribosomes have been studied in great detail, we know surprisingly little about the structural basis of mitochondrial protein synthesis. Here we used cryoelectron tomography and subtomogram analysis to visualize mitoribosomes in isolated yeast mitochondria, avoiding perturbations during ribosomal purification. Most mitoribosomes reside in immediate proximity to the inner mitochondrial membrane, in line with their specialization in the synthesis of hydrophobic membrane proteins. The subtomogram average of membrane-associated mitoribosomes reveals two distinct membrane contact sites, formed by the 21S rRNA expansion segment 96-ES1 and the inner membrane protein Mba1. On the basis of our data, we further hypothesize that Mba1 is not just a passive mitoribosome receptor on the inner membrane, but that it spatially aligns mitoribosomes with the membrane insertion machinery. This study reveals detailed insights into the supramolecular organization of the mitochondrial translation machinery and its association with the inner membrane in translation-competent mitochondria.

Suggested Citation

  • Stefan Pfeffer & Michael W. Woellhaf & Johannes M. Herrmann & Friedrich Förster, 2015. "Organization of the mitochondrial translation machinery studied in situ by cryoelectron tomography," Nature Communications, Nature, vol. 6(1), pages 1-8, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7019
    DOI: 10.1038/ncomms7019
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    Cited by:

    1. Florent Waltz & Thalia Salinas-Giegé & Robert Englmeier & Herrade Meichel & Heddy Soufari & Lauriane Kuhn & Stefan Pfeffer & Friedrich Förster & Benjamin D. Engel & Philippe Giegé & Laurence Drouard &, 2021. "How to build a ribosome from RNA fragments in Chlamydomonas mitochondria," Nature Communications, Nature, vol. 12(1), pages 1-15, December.

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