IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v20y2022i1p223-d1012940.html
   My bibliography  Save this article

Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli , Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal

Author

Listed:
  • Rita Batista

    (Food Microbiology Laboratory, Food and Nutrition Department, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal)

  • Margarida Saraiva

    (Food Microbiology Laboratory, Food and Nutrition Department, Rua Alexandre Herculano 321, 4000-055 Oporto, Portugal)

  • Teresa Lopes

    (Food Microbiology Laboratory, Food and Nutrition Department, Rua Alexandre Herculano 321, 4000-055 Oporto, Portugal)

  • Leonor Silveira

    (National Reference Laboratory for Gastrointestinal Infections, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal)

  • Anabela Coelho

    (Food Microbiology Laboratory, Food and Nutrition Department, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal)

  • Rosália Furtado

    (Food Microbiology Laboratory, Food and Nutrition Department, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal)

  • Rita Castro

    (National Reference Laboratory for Gastrointestinal Infections, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal)

  • Cristina Belo Correia

    (Food Microbiology Laboratory, Food and Nutrition Department, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal)

  • David Rodrigues

    (ESAC-IPC, Coimbra College of Agriculture, Polytechnic of Coimbra, 3045-601 Coimbra, Portugal
    CEF, Forest Research Centre, Edifício Prof. Azevedo Gomes, ISA, Tapada da Ajuda, 1349-017 Lisboa, Portugal)

  • Pedro Henriques

    (ESAC-IPC, Coimbra College of Agriculture, Polytechnic of Coimbra, 3045-601 Coimbra, Portugal
    Espaço de Visitação e Observação de Aves, 2600 Vila Franca de Xira, Portugal)

  • Sara Lóio

    (Centro de Recuperação de Fauna do Parque Biológico de Gaia, Rua da Cunha, Avintes, 4430-812 Vila Nova de Gaia, Portugal)

  • Vanessa Soeiro

    (Centro de Recuperação de Fauna do Parque Biológico de Gaia, Rua da Cunha, Avintes, 4430-812 Vila Nova de Gaia, Portugal)

  • Paulo Martins da Costa

    (ICBAS—Institute of Biomedical Sciences Abel Salazar, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Oporto, Portugal)

  • Mónica Oleastro

    (National Reference Laboratory for Gastrointestinal Infections, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal)

  • Angela Pista

    (National Reference Laboratory for Gastrointestinal Infections, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge, Avenida Padre Cruz, 1649-016 Lisbon, Portugal)

Abstract

Birds are potential carriers of pathogens affecting humans and agriculture. Aiming to evaluate the occurrence of the top three most important foodborne pathogens in free-living birds in Portugal, we investigated 108 individual fecal samples from free-living birds and one pooled sample of gull feces ( n = 50) for the presence of Escherichia coli (pathogenic and non-pathogenic), Salmonella spp. and Campylobacter spp. Virulence- and antimicrobial resistance- (AMR) associated genes were detected by PCR and Whole-Genome Sequencing (WGS), and phenotypic (serotyping and AMR profiles) characterization was performed. Overall, 8.9% of samples tested positive for pathogenic E. coli , 2.8% for Salmonella spp., and 9.9% for Campylobacter spp. AMR was performed on all pathogenic isolates and in a fraction of non-pathogenic E. coli , being detected in 25.9% of them. Ten of the tested E. coli isolates were multidrug-resistant (MDR), and seven of them were Extended-spectrum β-lactamase (ESBL) producers. Among Salmonella ( n = 3) and Campylobacter ( n = 9), only one strain of C. jejuni was identified as MDR. Most of the identified serotypes/sequence types had already been found to be associated with human disease. These results show that free-living birds in Portugal may act as carriers of foodborne pathogens linked to human disease, some of them resistant to critically important antimicrobials.

Suggested Citation

  • Rita Batista & Margarida Saraiva & Teresa Lopes & Leonor Silveira & Anabela Coelho & Rosália Furtado & Rita Castro & Cristina Belo Correia & David Rodrigues & Pedro Henriques & Sara Lóio & Vanessa Soe, 2022. "Genotypic and Phenotypic Characterization of Pathogenic Escherichia coli , Salmonella spp., and Campylobacter spp., in Free-Living Birds in Mainland Portugal," IJERPH, MDPI, vol. 20(1), pages 1-14, December.
    • Handle: RePEc:gam:jijerp:v:20:y:2022:i:1:p:223-:d:1012940
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/20/1/223/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/20/1/223/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Vanessa M. D’Costa & Christine E. King & Lindsay Kalan & Mariya Morar & Wilson W. L. Sung & Carsten Schwarz & Duane Froese & Grant Zazula & Fabrice Calmels & Regis Debruyne & G. Brian Golding & Hendri, 2011. "Antibiotic resistance is ancient," Nature, Nature, vol. 477(7365), pages 457-461, September.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Shahnawaz Hassan & Zulaykha Khurshid & Sabreena & Bikram Singh Bali & Bashir Ah Ganai & R. Z. Sayyed & Peter Poczai & Muzafar Zaman, 2022. "A Critical Assessment of the Congruency between Environmental DNA and Palaeoecology for the Biodiversity Monitoring and Palaeoenvironmental Reconstruction," IJERPH, MDPI, vol. 19(15), pages 1-25, August.
    2. Laura C. Scott & Nicholas Lee & Tiong Gim Aw, 2020. "Antibiotic Resistance in Minimally Human-Impacted Environments," IJERPH, MDPI, vol. 17(11), pages 1-12, June.
    3. Merja Ahonen & Anne Kahru & Angela Ivask & Kaja Kasemets & Siiri Kõljalg & Paride Mantecca & Ivana Vinković Vrček & Minna M. Keinänen-Toivola & Francy Crijns, 2017. "Proactive Approach for Safe Use of Antimicrobial Coatings in Healthcare Settings: Opinion of the COST Action Network AMiCI," IJERPH, MDPI, vol. 14(4), pages 1-23, March.
    4. Na Li & Hongna Li & Changxiong Zhu & Chong Liu & Guofeng Su & Jianguo Chen, 2022. "Controlling AMR in the Pig Industry: Is It Enough to Restrict Heavy Metals?," IJERPH, MDPI, vol. 19(18), pages 1-12, September.
    5. Asit Kumar Chakraborty, 2017. "Mechanisms of AMR: Mdr Genes and Antibiotics Decoys Retard the New Antibiotic Discovery against Superbugs," Novel Approaches in Drug Designing & Development, Juniper Publishers Inc., vol. 2(1), pages 1-5, June.
    6. Ming Ni & Antoine L Decrulle & Fanette Fontaine & Alice Demarez & Francois Taddei & Ariel B Lindner, 2012. "Pre-Disposition and Epigenetics Govern Variation in Bacterial Survival upon Stress," PLOS Genetics, Public Library of Science, vol. 8(12), pages 1-11, December.
    7. Na Li & Chong Liu & Zhiguo Zhang & Hongna Li & Tingting Song & Ting Liang & Binxu Li & Luyao Li & Shuo Feng & Qianqian Su & Jing Ye & Changxiong Zhu, 2019. "Research and Technological Advances Regarding the Study of the Spread of Antimicrobial Resistance Genes and Antimicrobial-Resistant Bacteria Related to Animal Husbandry," IJERPH, MDPI, vol. 16(24), pages 1-19, December.
    8. Iyiola Olatunji Oladunjoye & Yusuf Amuda Tajudeen & Habeebullah Jayeola Oladipo & Mona Said El-Sherbini, 2022. "Planetary Health and Traditional Medicine: A Potential Synergistic Approach to Tackle Antimicrobial Resistance," Challenges, MDPI, vol. 13(1), pages 1-10, June.
    9. Łukasz Jałowiecki & Jakub Hubeny & Monika Harnisz & Grażyna Płaza, 2021. "Seasonal and Technological Shifts of the WHO Priority Multi-Resistant Pathogens in Municipal Wastewater Treatment Plant and Its Receiving Surface Water: A Case Study," IJERPH, MDPI, vol. 19(1), pages 1-13, December.
    10. Stacy Slobodiuk & Caitlin Niven & Greer Arthur & Siddhartha Thakur & Ayse Ercumen, 2021. "Does Irrigation with Treated and Untreated Wastewater Increase Antimicrobial Resistance in Soil and Water: A Systematic Review," IJERPH, MDPI, vol. 18(21), pages 1-19, October.
    11. Milva Pepi & Silvano Focardi, 2021. "Antibiotic-Resistant Bacteria in Aquaculture and Climate Change: A Challenge for Health in the Mediterranean Area," IJERPH, MDPI, vol. 18(11), pages 1-31, May.
    12. Nolonwabo Nontongana & Timothy Sibanda & Elvis Ngwenya & Anthony I. Okoh, 2014. "Prevalence and Antibiogram Profiling of Escherichia coli Pathotypes Isolated from the Kat River and the Fort Beaufort Abstraction Water," IJERPH, MDPI, vol. 11(8), pages 1-15, August.
    13. Sharon P. Nappier & Krista Liguori & Audrey M. Ichida & Jill R. Stewart & Kaedra R. Jones, 2020. "Antibiotic Resistance in Recreational Waters: State of the Science," IJERPH, MDPI, vol. 17(21), pages 1-28, October.
    14. Valentin Duvauchelle & Elsa Causse & Julien Michon & Patrick Rateau & Karine Weiss & Patrick Meffre & Zohra Benfodda, 2021. "Evaluation of Knowledge and Risk Perception about Antibiotic Resistance in Biology and Mathematics Young Students in Nîmes University in France," IJERPH, MDPI, vol. 18(18), pages 1-12, September.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:20:y:2022:i:1:p:223-:d:1012940. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.