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QuEChERS LC–MS/MS Screening Method for Mycotoxin Detection in Cereal Products and Spices

Author

Listed:
  • Licia Pantano

    (Istituto Zooprofilattico Sperimentale della Sicilia, Via Gino Marinuzzi 3, 90129 Palermo, Italy)

  • Ladislao La Scala

    (Istituto Zooprofilattico Sperimentale della Sicilia, Via Gino Marinuzzi 3, 90129 Palermo, Italy)

  • Francesco Olibrio

    (Istituto Zooprofilattico Sperimentale della Sicilia, Via Gino Marinuzzi 3, 90129 Palermo, Italy)

  • Francesco Giuseppe Galluzzo

    (Istituto Zooprofilattico Sperimentale della Sicilia, Via Gino Marinuzzi 3, 90129 Palermo, Italy
    Dipartimento di Scienze della Vita, Università degli studi di Modena e Reggio Emilia, Via Università 4, 41121 Modena, Italy)

  • Carmelo Bongiorno

    (Istituto Zooprofilattico Sperimentale della Sicilia, Via Gino Marinuzzi 3, 90129 Palermo, Italy)

  • Maria Drussilla Buscemi

    (Istituto Zooprofilattico Sperimentale della Sicilia, Via Gino Marinuzzi 3, 90129 Palermo, Italy)

  • Andrea Macaluso

    (Istituto Zooprofilattico Sperimentale della Sicilia, Via Gino Marinuzzi 3, 90129 Palermo, Italy)

  • Antonio Vella

    (Istituto Zooprofilattico Sperimentale della Sicilia, Via Gino Marinuzzi 3, 90129 Palermo, Italy)

Abstract

We developed and validated a screening method for mycotoxin analysis in cereal products and spices. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC–MS/MS) was used for the analysis. Dispersive solid-phase extractions (d-SPEs) were used for the extraction of samples. Ochratoxin A (OTA), zearalenone (ZEA), aflatoxins (AFLA; AFB 1 , AFB 2 , AFG 1 , AFG 2 ), deoxynivalenol (DON), fumonisin (FUMO; FB 1 , FB 2 , FB 3 ), T2, and HT2 were validated in maize. AFLA and DON were validated in black pepper. The method satisfies the requirements of Commission Regulation (EC) no. 401/2006 and (EC) no. 1881/2006. The screening target concentration (STC) was under maximum permitted levels (MLs) for all mycotoxins validated. The method’s performance was assessed by two different proficiencies and tested with 100 real samples.

Suggested Citation

  • Licia Pantano & Ladislao La Scala & Francesco Olibrio & Francesco Giuseppe Galluzzo & Carmelo Bongiorno & Maria Drussilla Buscemi & Andrea Macaluso & Antonio Vella, 2021. "QuEChERS LC–MS/MS Screening Method for Mycotoxin Detection in Cereal Products and Spices," IJERPH, MDPI, vol. 18(7), pages 1-17, April.
    • Handle: RePEc:gam:jijerp:v:18:y:2021:i:7:p:3774-:d:530197
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    References listed on IDEAS

    as
    1. Noreddine Benkerroum, 2020. "Chronic and Acute Toxicities of Aflatoxins: Mechanisms of Action," IJERPH, MDPI, vol. 17(2), pages 1-28, January.
    2. Katarzyna Eufemia Przybyłowicz & Tomasz Arłukowicz & Anna Danielewicz & Jakub Morze & Magdalena Gajęcka & Łukasz Zielonka & Bartosz Fotschki & Tomasz Sawicki, 2020. "Association Between Mycotoxin Exposure and Dietary Habits in Colorectal Cancer Development Among a Polish Population: A Study Protocol," IJERPH, MDPI, vol. 17(3), pages 1-9, January.
    3. Ahmad Alshannaq & Jae-Hyuk Yu, 2017. "Occurrence, Toxicity, and Analysis of Major Mycotoxins in Food," IJERPH, MDPI, vol. 14(6), pages 1-20, June.
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