IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v13y2016i5p483-d69773.html
   My bibliography  Save this article

Effects of Size-Fractionated Particulate Matter on Cellular Oxidant Radical Generation in Human Bronchial Epithelial BEAS-2B Cells

Author

Listed:
  • Longfei Guan

    (Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China)

  • Wei Rui

    (Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China)

  • Ru Bai

    (Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China)

  • Wei Zhang

    (Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China)

  • Fang Zhang

    (Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China)

  • Wenjun Ding

    (Laboratory of Environment and Health, College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China)

Abstract

The aim of the present study was to investigate the effects of size-fractionated ( i.e. , <1; 1–2.5, and 2.5–10 µm in an aerodynamic diameter) ambient particulate matter (PM) on reactive oxygen species (ROS) activity and cell viability in human bronchial epithelial cells (BEAS-2B). The PM samples were collected from an urban site (uPM) in Beijing and a steel factory site (sPM) in Anshan, China, from March 2013 to December 2014. Metal elements, organic and elemental carbon, and water-soluble inorganic ions in the uPM and sPM were analyzed. The cell viability and ROS generation in PM-exposed BEAS-2B cells were measured by MTS and DCFH-DA. The results showed that both uPM and sPM caused a decrease in the cell viability and an increase in ROS generation. The level of ROS measured in sPM 1.0 was approximately triple that in uPM 1.0 . The results of correlation analysis showed that the ROS activity and cytotoxicity were related to different PM composition. Moreover, deferoxamine (DFO) significantly prevented the increase of ROS generation and the decrease of cell viability. Taken together, our results suggest that the metals absorbed on PM induced oxidant radical generation in BEAS-2B cells that could lead to impairment of pulmonary function.

Suggested Citation

  • Longfei Guan & Wei Rui & Ru Bai & Wei Zhang & Fang Zhang & Wenjun Ding, 2016. "Effects of Size-Fractionated Particulate Matter on Cellular Oxidant Radical Generation in Human Bronchial Epithelial BEAS-2B Cells," IJERPH, MDPI, vol. 13(5), pages 1-14, May.
    • Handle: RePEc:gam:jijerp:v:13:y:2016:i:5:p:483-:d:69773
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/13/5/483/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/13/5/483/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Ru-Jin Huang & Yanlin Zhang & Carlo Bozzetti & Kin-Fai Ho & Jun-Ji Cao & Yongming Han & Kaspar R. Daellenbach & Jay G. Slowik & Stephen M. Platt & Francesco Canonaco & Peter Zotter & Robert Wolf & Sim, 2014. "High secondary aerosol contribution to particulate pollution during haze events in China," Nature, Nature, vol. 514(7521), pages 218-222, October.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Ao Di & Yun Wu & Mindong Chen & Dongyang Nie & Xinlei Ge, 2020. "Chemical Characterization of Seasonal PM 2.5 Samples and Their Cytotoxicity in Human Lung Epithelial Cells (A549)," IJERPH, MDPI, vol. 17(12), pages 1-13, June.
    2. Yun Wu & Mei Wang & Shaojuan Luo & Yunfeng Gu & Dongyang Nie & Zhiyang Xu & Yue Wu & Mindong Chen & Xinlei Ge, 2020. "Comparative Toxic Effects of Manufactured Nanoparticles and Atmospheric Particulate Matter in Human Lung Epithelial Cells," IJERPH, MDPI, vol. 18(1), pages 1-12, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Jing Zhang & Wenjian Zhu & Dubin Dong & Yuan Ren & Wenhao Hu & Xinjie Jin & Zhengxuan He & Jian Chen & Xiaoai Jin & Tianhuan Zhou, 2024. "The Influence of Three-Dimensional Building Morphology on PM 2.5 Concentrations in the Yangtze River Delta," Sustainability, MDPI, vol. 16(17), pages 1-17, August.
    2. Shi, Wenxiao & Lin, Chen & Chen, Wei & Hong, Jinglan & Chang, Jingcai & Dong, Yong & Zhang, Yanlu, 2017. "Environmental effect of current desulfurization technology on fly dust emission in China," Renewable and Sustainable Energy Reviews, Elsevier, vol. 72(C), pages 1-9.
    3. Yi Yang & Jie Li & Guobin Zhu & Qiangqiang Yuan, 2019. "Spatio–Temporal Relationship and Evolvement of Socioeconomic Factors and PM 2.5 in China During 1998–2016," IJERPH, MDPI, vol. 16(7), pages 1-24, March.
    4. Zhixiong Tan & Haili Wu & Qingyang Chen & Jiejun Huang, 2024. "Spatiotemporal Analysis of Air Quality and Its Driving Factors in Beijing’s Main Urban Area," Sustainability, MDPI, vol. 16(14), pages 1-18, July.
    5. Yana Jin & Henrik Andersson & Shiqiu Zhang, 2016. "Air Pollution Control Policies in China: A Retrospective and Prospects," IJERPH, MDPI, vol. 13(12), pages 1-22, December.
    6. Lili Guo & Yuting Song & Mengqian Tang & Jinyang Tang & Bright Senyo Dogbe & Mengying Su & Houjian Li, 2022. "Assessing the Relationship among Land Transfer, Fertilizer Usage, and PM 2.5 Pollution: Evidence from Rural China," IJERPH, MDPI, vol. 19(14), pages 1-18, July.
    7. Yu Zhang & Jiayu Wu & Chunyao Zhou & Qingyu Zhang, 2019. "Installation Planning in Regional Thermal Power Industry for Emissions Reduction Based on an Emissions Inventory," IJERPH, MDPI, vol. 16(6), pages 1-13, March.
    8. Hujia Zhao & Ke Gui & Yanjun Ma & Yangfeng Wang & Yaqiang Wang & Hong Wang & Yu Zheng & Lei Li & Lei Zhang & Yuqi Zhang & Huizheng Che & Xiaoye Zhang, 2022. "Multi-Year Variation of Ozone and Particulate Matter in Northeast China Based on the Tracking Air Pollution in China (TAP) Data," IJERPH, MDPI, vol. 19(7), pages 1-23, March.
    9. Ruiqing Ma & Yeyue Zhang & Yini Zhang & Xi Li & Zheng Ji, 2023. "The Relationship between the Transmission of Different SARS-CoV-2 Strains and Air Quality: A Case Study in China," IJERPH, MDPI, vol. 20(3), pages 1-17, January.
    10. Hongfeng Zhang & Lu Huang & Yan Zhu & Hongyun Si & Xu He, 2021. "Does Low-Carbon City Construction Improve Total Factor Productivity? Evidence from a Quasi-Natural Experiment in China," IJERPH, MDPI, vol. 18(22), pages 1-21, November.
    11. Han-Yin Sun & Ci-Wen Luo & Yun-Wei Chiang & Kun-Lin Yeh Yi-Ching Li & Yung-Chung Ho & Shiuan-Shinn Lee & Wen-Ying Chen & Chun-Jung Chen & Yu-Hsiang Kuan, 2021. "Association Between PM 2.5 Exposure Level and Primary Open-Angle Glaucoma in Taiwanese Adults: A Nested Case–control Study," IJERPH, MDPI, vol. 18(4), pages 1-12, February.
    12. Zhang, Dongyang, 2023. "Can environmental monitoring power transition curb corporate greenwashing behavior?," Journal of Economic Behavior & Organization, Elsevier, vol. 212(C), pages 199-218.
    13. Ying Zhang & Shouming Chen & Yujia Li & Disney Leite Ramos, 2024. "Does Environmental Protection Law Bring about Greenwashing? Evidence from Heavy-Polluting Firms in China," Sustainability, MDPI, vol. 16(5), pages 1-20, February.
    14. Zhong, Yu-Xiu & Wang, Xin & Xu, Gang & Ning, Xinyu & Zhou, Lin & Tang, Wen & Wang, Ming-Hao & Wang, Tong & Xu, Jun & Jiang, Long & Wang, Yi & Su, Sheng & Hu, Song & Xiang, Jun, 2023. "Investigation on slagging and high-temperature corrosion prevention and control of a 1000 MW ultra supercritical double tangentially fired boiler," Energy, Elsevier, vol. 275(C).
    15. Shr, Yau-Huo & Hsu, Wen & Hwang, Bing-Fang & Jung, Chau-Ren, 2023. "Air quality and risky behaviors on roads," Journal of Environmental Economics and Management, Elsevier, vol. 118(C).
    16. Jie Yang & Pengfei Liu & Hongquan Song & Changhong Miao & Feng Wang & Yu Xing & Wenjie Wang & Xinyu Liu & Mengxin Zhao, 2021. "Effects of Anthropogenic Emissions from Different Sectors on PM 2.5 Concentrations in Chinese Cities," IJERPH, MDPI, vol. 18(20), pages 1-13, October.
    17. Kun Liu & Xuemin Liu & Zihao Wu, 2024. "Nexus between Corporate Digital Transformation and Green Technological Innovation Performance: The Mediating Role of Optimizing Resource Allocation," Sustainability, MDPI, vol. 16(3), pages 1-21, February.
    18. Diyi Liu & Kun Cheng & Kevin Huang & Hui Ding & Tiantong Xu & Zhenni Chen & Yanqi Sun, 2022. "Visualization and Analysis of Air Pollution and Human Health Based on Cluster Analysis: A Bibliometric Review from 2001 to 2021," IJERPH, MDPI, vol. 19(19), pages 1-15, October.
    19. Eun-Min Cho & Hyung Jin Jeon & Dan Ki Yoon & Si Hyun Park & Hyung Jin Hong & Kil Yong Choi & Heun Woo Cho & Hyo Chang Cheon & Cheol Min Lee, 2019. "Reliability of Low-Cost, Sensor-Based Fine Dust Measurement Devices for Monitoring Atmospheric Particulate Matter Concentrations," IJERPH, MDPI, vol. 16(8), pages 1-10, April.
    20. Qi Chen & Ruqian Miao & Guannan Geng & Manish Shrivastava & Xu Dao & Bingye Xu & Jiaqi Sun & Xian Zhang & Mingyuan Liu & Guigang Tang & Qian Tang & Hanwen Hu & Ru-Jin Huang & Hao Wang & Yan Zheng & Yu, 2024. "Widespread 2013-2020 decreases and reduction challenges of organic aerosol in China," Nature Communications, Nature, vol. 15(1), pages 1-8, December.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:13:y:2016:i:5:p:483-:d:69773. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.