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A Biofuel Similar to Biodiesel Obtained by Using a Lipase from Rhizopus oryzae , Optimized by Response Surface Methodology

Author

Listed:
  • Carlos Luna

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain)

  • Cristobal Verdugo

    (Laboratorio de Estudios Cristalográficos, IACT, CSIC-University of Granada, Avenida de las Palmeras 4, 18100 Armilla, Granada, Spain)

  • Enrique D. Sancho

    (Department of Microbiology, University of Córdoba, Campus de Rabanales, Ed. Severo Ochoa, 14014 Córdoba, Spain)

  • Diego Luna

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain
    Seneca Green Catalyst S.L., Campus de Rabanales, 14014 Córdoba, Spain)

  • Juan Calero

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain)

  • Alejandro Posadillo

    (Seneca Green Catalyst S.L., Campus de Rabanales, 14014 Córdoba, Spain)

  • Felipa M. Bautista

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain)

  • Antonio A. Romero

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie,14014 Córdoba, Spain)

Abstract

A new biodiesel-like biofuel is obtained by the enzymatic ethanolysis reaction of sunflower oil with ethanol, in free solvent media, by using BIOLIPASE-R, a multipurpose alimentary additive from Biocon ® -Spain that is a low cost lipase from a strain of Rhizopus oryzae . This biofuel is composed by two parts of fatty acid ethyl esters (FAEE) and one of monoglyceride (MG), which in this form integrates glycerol, through the application of the 1,3-selective lipases. Thus, this process minimizes waste generation and maximizes the efficiency of the process because no residual glycerol is produced. Response surface methodology (RSM) is employed to evaluate the main reaction parameters (reaction temperature, oil/ethanol ratio and pH) on the sunflower oil conversion. Water content and amount of lipase were also previously investigated. Regarding the results, we found that it operates optimally with a water content of the reaction medium of 0.15%, 0.05%–0.1% lipase by weight relative to the weight of oil used, 20 °C, volume ratio (mL/mL) oil/ethanol 12/3.5 and pH 12 (by addition of 50 µL of 10 N NaOH solution). These results have proven a very good efficiency of the biocatalyst in the studied selective process.

Suggested Citation

  • Carlos Luna & Cristobal Verdugo & Enrique D. Sancho & Diego Luna & Juan Calero & Alejandro Posadillo & Felipa M. Bautista & Antonio A. Romero, 2014. "A Biofuel Similar to Biodiesel Obtained by Using a Lipase from Rhizopus oryzae , Optimized by Response Surface Methodology," Energies, MDPI, vol. 7(5), pages 1-17, May.
    • Handle: RePEc:gam:jeners:v:7:y:2014:i:5:p:3383-3399:d:36323
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    References listed on IDEAS

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    1. Haseeb, A.S.M.A. & Sia, S.Y. & Fazal, M.A. & Masjuki, H.H., 2010. "Effect of temperature on tribological properties of palm biodiesel," Energy, Elsevier, vol. 35(3), pages 1460-1464.
    2. Demirbas, Ayhan, 2009. "Political, economic and environmental impacts of biofuels: A review," Applied Energy, Elsevier, vol. 86(Supplemen), pages 108-117, November.
    3. Oh, Pin Pin & Lau, Harrison Lik Nang & Chen, Junghui & Chong, Mei Fong & Choo, Yuen May, 2012. "A review on conventional technologies and emerging process intensification (PI) methods for biodiesel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 16(7), pages 5131-5145.
    4. Xu, Yufu & Wang, Qiongjie & Hu, Xianguo & Li, Chuan & Zhu, Xifeng, 2010. "Characterization of the lubricity of bio-oil/diesel fuel blends by high frequency reciprocating test rig," Energy, Elsevier, vol. 35(1), pages 283-287.
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    Cited by:

    1. Juan Calero & Diego Luna & Carlos Luna & Felipa M. Bautista & Beatriz Hurtado & Antonio A. Romero & Alejandro Posadillo & Rafael Estevez, 2019. "Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology," Energies, MDPI, vol. 12(5), pages 1-15, March.
    2. Rafael Estevez & Laura Aguado-Deblas & Francisco J. López-Tenllado & Carlos Luna & Juan Calero & Antonio A. Romero & Felipa M. Bautista & Diego Luna, 2022. "Biodiesel Is Dead: Long Life to Advanced Biofuels—A Comprehensive Critical Review," Energies, MDPI, vol. 15(9), pages 1-39, April.

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