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Biofuel that Keeps Glycerol as Monoglyceride by 1,3-Selective Ethanolysis with Pig Pancreatic Lipase Covalently Immobilized on AlPO 4 Support

Author

Listed:
  • Carlos Luna

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain)

  • Enrique Sancho

    (Department of Microbiology, University of Córdoba, Campus de Rabanales, Ed. Severo Ochoa, 14014 Córdoba, Spain)

  • Diego Luna

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain)

  • Verónica Caballero

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain)

  • Juan Calero

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain)

  • Alejandro Posadillo

    (Seneca Green Catalyst S.L., Campus de Rabanales, 14014 Córdoba, Spain)

  • Cristóbal Verdugo

    (Crystallographic Studies Laboratory, Andalusian Institute of Earth Sciences, CSIC, Avda. Las Palmeras nº4, 18100 Armilla, Granada, Spain)

  • Felipa M. Bautista

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain)

  • Antonio A. Romero

    (Department of Organic Chemistry, University of Cordoba, Campus de Rabanales, Ed. Marie Curie, 14014 Córdoba, Spain)

Abstract

By using pig pancreatic lipase (EC 3.1.1.3 or PPL) as a biocatalyst, covalently immobilized on amorphous AlPO 4 support, a new second generation biodiesel was obtained in the transesterification reaction of sunflower oil with ethanol. The resulting biofuel is composed of fatty acid ethyl esters and monoglycerides (FAEE/MG) blended in a 2:1 molar ratio. This novel product, which integrates glycerol as monoacylglycerols (MG) into the biofuels composition, has similar physicochemical properties as conventional biodiesel and also avoids the removal step of the by-product by washing of the biodiesel with water. Immobilization of PPL was achieved by covalent attachment of the ε -amino group of the lysine residues of PPL with the aldehyde groups of p -hydroxybenzaldehyde linked on a hybrid organic-inorganic functionalized AlPO 4 surface. With this procedure, the PPL biocatalyst was strongly fixed to the inorganic support surface (94.3%). Nevertheless, the efficiency of the immobilized enzyme was relatively lower compared to that of the free PPL, but it showed a remarkable stability as well as a great capacity of reutilization (25 reuses) without a significant loss of its initial catalytic activity. Therefore, this enzymatic method allows the production of a biodiesel which integrates the glycerol, allows a more efficient fabrication method and minimizes the waste production as compared to the conventional alkali-catalyzed process.

Suggested Citation

  • Carlos Luna & Enrique Sancho & Diego Luna & Verónica Caballero & Juan Calero & Alejandro Posadillo & Cristóbal Verdugo & Felipa M. Bautista & Antonio A. Romero, 2013. "Biofuel that Keeps Glycerol as Monoglyceride by 1,3-Selective Ethanolysis with Pig Pancreatic Lipase Covalently Immobilized on AlPO 4 Support," Energies, MDPI, vol. 6(8), pages 1-22, July.
    • Handle: RePEc:gam:jeners:v:6:y:2013:i:8:p:3879-3900:d:27600
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    References listed on IDEAS

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    Cited by:

    1. Guimarães, José Renato & Fernandez-Lafuente, Roberto & Tardioli, Paulo Waldir, 2022. "Ethanolysis of soybean oil catalyzed by magnetic CLEA of porcine pancreas lipase to produce ecodiesel. Efficient separation of ethyl esters and monoglycerides," Renewable Energy, Elsevier, vol. 198(C), pages 455-462.
    2. Dutra, Luciana da Silva & Costa Cerqueira Pinto, Martina & Cipolatti, Eliane Pereira & Aguieiras, Erika Cristina G. & Manoel, Evelin Andrade & Greco-Duarte, Jaqueline & Guimarães Freire, Denise Maria , 2022. "How the biodiesel from immobilized enzymes production is going on: An advanced bibliometric evaluation of global research," Renewable and Sustainable Energy Reviews, Elsevier, vol. 153(C).
    3. Juan Calero & Diego Luna & Carlos Luna & Felipa M. Bautista & Beatriz Hurtado & Antonio A. Romero & Alejandro Posadillo & Rafael Estevez, 2019. "Rhizomucor miehei Lipase Supported on Inorganic Solids, as Biocatalyst for the Synthesis of Biofuels: Improving the Experimental Conditions by Response Surface Methodology," Energies, MDPI, vol. 12(5), pages 1-15, March.
    4. Rafael Estevez & Laura Aguado-Deblas & Francisco J. López-Tenllado & Carlos Luna & Juan Calero & Antonio A. Romero & Felipa M. Bautista & Diego Luna, 2022. "Biodiesel Is Dead: Long Life to Advanced Biofuels—A Comprehensive Critical Review," Energies, MDPI, vol. 15(9), pages 1-39, April.
    5. Calero, Juan & Luna, Diego & Sancho, Enrique D. & Luna, Carlos & Bautista, Felipa M. & Romero, Antonio A. & Posadillo, Alejandro & Berbel, Julio & Verdugo-Escamilla, Cristóbal, 2015. "An overview on glycerol-free processes for the production of renewable liquid biofuels, applicable in diesel engines," Renewable and Sustainable Energy Reviews, Elsevier, vol. 42(C), pages 1437-1452.

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