IDEAS home Printed from https://ideas.repec.org/a/gam/jeners/v14y2021i15p4443-d599786.html
   My bibliography  Save this article

Screening of Fungal Strains for Cellulolytic and Xylanolytic Activities Production and Evaluation of Brewers’ Spent Grain as Substrate for Enzyme Production by Selected Fungi

Author

Listed:
  • Rossana Liguori

    (Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy)

  • Anna Pennacchio

    (Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy)

  • Luciana Porto de Souza Vandenberghe

    (Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná Coronel Francisco H. Dos Santos Avenue, 210, Curitiba 81531-980, Brazil)

  • Addolorata De Chiaro

    (Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy)

  • Leila Birolo

    (Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy)

  • Carlos Ricardo Soccol

    (Department of Bioprocess Engineering and Biotechnology, Federal University of Paraná Coronel Francisco H. Dos Santos Avenue, 210, Curitiba 81531-980, Brazil)

  • Vincenza Faraco

    (Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy)

Abstract

Brewer’s spent grain (BSG), the solid residue of beer production, is attracting significant attention as raw material for the production of added value substances, since until recently it was mainly used as animal feed or deposited in landfills, causing serious environmental problems. Therefore, this work aimed at developing a bioprocess using BSG as a substrate for the production of cellulases and xylanases for waste saccharification and bioenergy production. Different fungi were analyzed for their cellulolytic and xylanolytic abilities, through a first screening on solid media by assessment of fungal growth and enzyme production on agar containing carboxylmethylcellulose or xylan as the sole carbon source, respectively. The best cellulase and xylanase producers were subjected to quantitative evaluation of enzyme production in liquid cultures. Aspergillus niger LPB-334 was selected for its ability to produce cellulase and xylanase at high levels and it was cultivated on BSG by solid state fermentation. The cellulase production reached a maximum of 118.04 ± 8.4 U/g of dry substrate after 10 days of fermentation, while a maximum xylanase production of 1315.15 ± 37.5 U/g of dry substrate was reached after 4 days. Preliminary characterization of cellulase and xylanase activities and identification of the enzymes responsible were carried out.

Suggested Citation

  • Rossana Liguori & Anna Pennacchio & Luciana Porto de Souza Vandenberghe & Addolorata De Chiaro & Leila Birolo & Carlos Ricardo Soccol & Vincenza Faraco, 2021. "Screening of Fungal Strains for Cellulolytic and Xylanolytic Activities Production and Evaluation of Brewers’ Spent Grain as Substrate for Enzyme Production by Selected Fungi," Energies, MDPI, vol. 14(15), pages 1-17, July.
    • Handle: RePEc:gam:jeners:v:14:y:2021:i:15:p:4443-:d:599786
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1996-1073/14/15/4443/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1996-1073/14/15/4443/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Rossana Liguori & Carlos Ricardo Soccol & Luciana Porto de Souza Vandenberghe & Adenise Lorenci Woiciechowski & Vincenza Faraco, 2015. "Second Generation Ethanol Production from Brewers’ Spent Grain," Energies, MDPI, vol. 8(4), pages 1-12, March.
    2. Amore, Antonella & Faraco, Vincenza, 2012. "Potential of fungi as category I Consolidated BioProcessing organisms for cellulosic ethanol production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 16(5), pages 3286-3301.
    3. Richard Ahorsu & Francesc Medina & Magda Constantí, 2018. "Significance and Challenges of Biomass as a Suitable Feedstock for Bioenergy and Biochemical Production: A Review," Energies, MDPI, vol. 11(12), pages 1-19, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Danilo Arcentales-Bastidas & Carla Silva & Angel D. Ramirez, 2022. "The Environmental Profile of Ethanol Derived from Sugarcane in Ecuador: A Life Cycle Assessment Including the Effect of Cogeneration of Electricity in a Sugar Industrial Complex," Energies, MDPI, vol. 15(15), pages 1-24, July.
    2. Tuan Hoang, Anh & Viet Pham, Van, 2021. "2-Methylfuran (MF) as a potential biofuel: A thorough review on the production pathway from biomass, combustion progress, and application in engines," Renewable and Sustainable Energy Reviews, Elsevier, vol. 148(C).
    3. Mariana S. T. Amândio & Joana M. Pereira & Jorge M. S. Rocha & Luísa S. Serafim & Ana M. R. B. Xavier, 2022. "Getting Value from Pulp and Paper Industry Wastes: On the Way to Sustainability and Circular Economy," Energies, MDPI, vol. 15(11), pages 1-31, June.
    4. Bogusława Waliszewska & Mieczysław Grzelak & Eliza Gaweł & Agnieszka Spek-Dźwigała & Agnieszka Sieradzka & Wojciech Czekała, 2021. "Chemical Characteristics of Selected Grass Species from Polish Meadows and Their Potential Utilization for Energy Generation Purposes," Energies, MDPI, vol. 14(6), pages 1-14, March.
    5. Yichen Liu & James J. Leahy & Jacek Grams & Witold Kwapinski, 2019. "Hydro-Pyrolysis and Catalytic Upgrading of Biomass and Its Hydroxy Residue Fast Pyrolysis Vapors," Energies, MDPI, vol. 12(18), pages 1-18, September.
    6. Małgorzata Hawrot-Paw & Aleksander Stańczuk, 2022. "From Waste Biomass to Cellulosic Ethanol by Separate Hydrolysis and Fermentation (SHF) with Trichoderma viride," Sustainability, MDPI, vol. 15(1), pages 1-10, December.
    7. Sahar Safarian & Seyed Mohammad Ebrahimi Saryazdi & Runar Unnthorsson & Christiaan Richter, 2021. "Artificial Neural Network Modeling of Bioethanol Production Via Syngas Fermentation," Biophysical Economics and Resource Quality, Springer, vol. 6(1), pages 1-13, March.
    8. Beata Brzychczyk & Tomasz Hebda & Norbert Pedryc, 2020. "The Influence of Artificial Lighting Systems on the Cultivation of Algae: The Example of Chlorella vulgaris," Energies, MDPI, vol. 13(22), pages 1-14, November.
    9. Roksana Muzyka & Szymon Sobek & Mariusz Dudziak & Miloud Ouadi & Marcin Sajdak, 2023. "A Comparative Analysis of Waste Biomass Pyrolysis in Py-GC-MS and Fixed-Bed Reactors," Energies, MDPI, vol. 16(8), pages 1-15, April.
    10. Takahiro Nakashima & Keiichiro Ueno & Eisuke Fujita & Shoko Ishikawa, 2020. "Evaluation of Polyethylene Mulching and Sugarcane Cultivar on Energy Inputs and Greenhouse Gas Emissions for Ethanol Production in a Temperate Climate," Energies, MDPI, vol. 13(17), pages 1-17, August.
    11. Sofía Sampaolesi & Laura Estefanía Briand & Mario Carlos Nazareno Saparrat & María Victoria Toledo, 2023. "Potentials of Biomass Waste Valorization: Case of South America," Sustainability, MDPI, vol. 15(10), pages 1-21, May.
    12. Daissy Lorena Restrepo-Serna & Jimmy Anderson Martínez-Ruano & Carlos Ariel Cardona-Alzate, 2018. "Energy Efficiency of Biorefinery Schemes Using Sugarcane Bagasse as Raw Material," Energies, MDPI, vol. 11(12), pages 1-12, December.
    13. Mattias, Gaglio & Elena, Tamburini & Giuseppe, Castaldelli & Anna, Fano Elisa, 2021. "Modeling the ecosystem service of agricultural residues provision for bioenergy production: A potential application in the Emilia-Romagna region (Italy)," Ecological Modelling, Elsevier, vol. 451(C).
    14. Siddique, Istiaq Jamil & Salema, Arshad Adam & Antunes, Elsa & Vinu, Ravikrishnan, 2022. "Technical challenges in scaling up the microwave technology for biomass processing," Renewable and Sustainable Energy Reviews, Elsevier, vol. 153(C).
    15. Aditiya, H.B. & Mahlia, T.M.I. & Chong, W.T. & Nur, Hadi & Sebayang, A.H., 2016. "Second generation bioethanol production: A critical review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 66(C), pages 631-653.
    16. Ong, Victor Zhenquan & Wu, Ta Yeong, 2020. "An application of ultrasonication in lignocellulosic biomass valorisation into bio-energy and bio-based products," Renewable and Sustainable Energy Reviews, Elsevier, vol. 132(C).
    17. Ibrahim, Nur Atirah & Wan Alwi, Sharifah Rafidah & Manan, Zainuddin Abdul & Mustaffa, Azizul Azri & Kidam, Kamarizan, 2022. "Risk matrix approach of extreme temperature and precipitation for renewable energy systems in Malaysia," Energy, Elsevier, vol. 254(PC).
    18. Mateusz Jackowski & Lukasz Niedzwiecki & Magdalena Lech & Mateusz Wnukowski & Amit Arora & Monika Tkaczuk-Serafin & Marcin Baranowski & Krystian Krochmalny & Vivek K. Veetil & Przemysław Seruga & Anna, 2020. "HTC of Wet Residues of the Brewing Process: Comprehensive Characterization of Produced Beer, Spent Grain and Valorized Residues," Energies, MDPI, vol. 13(8), pages 1-20, April.
    19. Sedef E. Kara & Mustapha D. Ibrahim & Sahand Daneshvar, 2021. "Dual Efficiency and Productivity Analysis of Renewable Energy Alternatives of OECD Countries," Sustainability, MDPI, vol. 13(13), pages 1-14, July.
    20. Wagner, Evelyn & Sierra-Ibarra, Estefanía & Rojas, Natalia L. & Martinez, Alfredo, 2022. "One-pot bioethanol production from brewery spent grain using the ethanologenic Escherichia coli MS04," Renewable Energy, Elsevier, vol. 189(C), pages 717-725.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jeners:v:14:y:2021:i:15:p:4443-:d:599786. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.