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Novel Yeast Strains for the Efficient Saccharification and Fermentation of Starchy By-Products to Bioethanol

Author

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  • Nicoletta Gronchi

    (Department of Agronomy, Food, Natural resources, Animals and the Environment (DAFNAE), University of Padova, Agripolis, 35020 Legnaro (PD), Italy)

  • Lorenzo Favaro

    (Department of Agronomy, Food, Natural resources, Animals and the Environment (DAFNAE), University of Padova, Agripolis, 35020 Legnaro (PD), Italy)

  • Lorenzo Cagnin

    (Department of Agronomy, Food, Natural resources, Animals and the Environment (DAFNAE), University of Padova, Agripolis, 35020 Legnaro (PD), Italy)

  • Silvia Brojanigo

    (Department of Agronomy, Food, Natural resources, Animals and the Environment (DAFNAE), University of Padova, Agripolis, 35020 Legnaro (PD), Italy)

  • Valentino Pizzocchero

    (Department of Agronomy, Food, Natural resources, Animals and the Environment (DAFNAE), University of Padova, Agripolis, 35020 Legnaro (PD), Italy)

  • Marina Basaglia

    (Department of Agronomy, Food, Natural resources, Animals and the Environment (DAFNAE), University of Padova, Agripolis, 35020 Legnaro (PD), Italy)

  • Sergio Casella

    (Department of Agronomy, Food, Natural resources, Animals and the Environment (DAFNAE), University of Padova, Agripolis, 35020 Legnaro (PD), Italy)

Abstract

The use of solid starchy waste streams to produce value-added products, such as fuel ethanol, is a priority for the global bio-based economy. Despite technological advances, bioethanol production from starch is still not economically competitive. Large cost-savings can be achieved through process integration (consolidated bioprocessing, CBP) and new amylolytic microbes that are able to directly convert starchy biomass into fuel in a single bioreactor. Firstly, CBP technology requires efficient fermenting yeast strains to be engineered for amylase(s) production. This study addressed the selection of superior yeast strains with high fermentative performances to be used as recipient for future CBP engineering of fungal amylases. Twenty-one newly isolated wild-type Saccharomyces cerevisiae strains were screened at 30 °C in a simultaneous saccharification and fermentation (SSF) set up using starchy substrates at high loading (20% w/v) and the commercial amylases cocktail STARGEN™ 002. The industrial yeast Ethanol Red™ was used as benchmark. A cluster of strains produced ethanol levels (up to 118 g/L) significantly higher than those of Ethanol Red™ (about 109 g/L). In particular, S. cerevisiae L20, selected for a scale-up process into a 1-L bioreactor, confirmed the outstanding performance over the industrial benchmark, producing nearly 101 g/L ethanol instead of 94 g/L. As a result, this strain can be a promising CBP host for heterologous expression of fungal amylases towards the design of novel and efficient starch-to-ethanol routes.

Suggested Citation

  • Nicoletta Gronchi & Lorenzo Favaro & Lorenzo Cagnin & Silvia Brojanigo & Valentino Pizzocchero & Marina Basaglia & Sergio Casella, 2019. "Novel Yeast Strains for the Efficient Saccharification and Fermentation of Starchy By-Products to Bioethanol," Energies, MDPI, vol. 12(4), pages 1-13, February.
    • Handle: RePEc:gam:jeners:v:12:y:2019:i:4:p:714-:d:208145
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    References listed on IDEAS

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    3. Poomani, Merlin Sobia & Mariappan, Iyyadurai & Muthan, Krishnaveni & Subramanian, Venkatesh, 2024. "Insights of Pichia kudriavzevii SVMS2019 for cellulase production and fermentation into ethanol," Renewable Energy, Elsevier, vol. 225(C).
    4. Rosen, Yan & Mamane, Hadas & Gerchman, Yoram, 2021. "Immersed ozonation of agro-wastes as an effective pretreatment method in bioethanol production," Renewable Energy, Elsevier, vol. 174(C), pages 382-390.
    5. Gupte, Ameya Pankaj & Basaglia, Marina & Casella, Sergio & Favaro, Lorenzo, 2022. "Rice waste streams as a promising source of biofuels: feedstocks, biotechnologies and future perspectives," Renewable and Sustainable Energy Reviews, Elsevier, vol. 167(C).
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