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Comparison of steam-alkali-chemical and microwave-alkali pretreatment for enhancing the enzymatic saccharification of oil palm trunk

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  • Lai, Long Wee
  • Idris, Ani

Abstract

This paper demonstrates two different pretreatment protocols for oil palm trunks (OPT); steam-alkali-chemical (SAC) and microwave-alkali (Mw-A) method. The composition, morphology, structure and crystallinity of OPT before and after pretreatment were analyzed. The effectiveness of the pretreated methods was investigated by performing enzymatic saccharification on the OPT. The physiochemical factors namely: enzyme ratio (cellulase to β-glucosidase), pH, temperature and substrate loading (w/v) on enzymatic saccharification were also investigated. The pre-determined optimal conditions were then used for further enzymatic hydrolysis of raw and pretreated OPT substrates. The results revealed a huge degree of reduction in lignin, up to 89% for SAC treated OPT and at least 15% for Mw-A treated OPT sample as compared to untreated ones. High glucose accumulation (79.4%) was obtained after 72 h saccharification for both pretreated OPT samples.

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  • Lai, Long Wee & Idris, Ani, 2016. "Comparison of steam-alkali-chemical and microwave-alkali pretreatment for enhancing the enzymatic saccharification of oil palm trunk," Renewable Energy, Elsevier, vol. 99(C), pages 738-746.
    • Handle: RePEc:eee:renene:v:99:y:2016:i:c:p:738-746
    DOI: 10.1016/j.renene.2016.07.059
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    1. Awalludin, Mohd Fahmi & Sulaiman, Othman & Hashim, Rokiah & Nadhari, Wan Noor Aidawati Wan, 2015. "An overview of the oil palm industry in Malaysia and its waste utilization through thermochemical conversion, specifically via liquefaction," Renewable and Sustainable Energy Reviews, Elsevier, vol. 50(C), pages 1469-1484.
    2. Binod, Parameswaran & Satyanagalakshmi, Karri & Sindhu, Raveendran & Janu, Kanakambaran Usha & Sukumaran, Rajeev K. & Pandey, Ashok, 2012. "Short duration microwave assisted pretreatment enhances the enzymatic saccharification and fermentable sugar yield from sugarcane bagasse," Renewable Energy, Elsevier, vol. 37(1), pages 109-116.
    3. Coimbra, Michelle Cardoso & Duque, Aleta & Saéz, Felicia & Manzanares, Paloma & Garcia-Cruz, Crispin Humberto & Ballesteros, Mercedes, 2016. "Sugar production from wheat straw biomass by alkaline extrusion and enzymatic hydrolysis," Renewable Energy, Elsevier, vol. 86(C), pages 1060-1068.
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    2. Ahmad, Farah B. & Zhang, Zhanying & Doherty, William O.S. & O'Hara, Ian M., 2019. "The outlook of the production of advanced fuels and chemicals from integrated oil palm biomass biorefinery," Renewable and Sustainable Energy Reviews, Elsevier, vol. 109(C), pages 386-411.
    3. Patricia Portero-Barahona & Enrique Javier Carvajal-Barriga & Jesús Martín-Gil & Pablo Martín-Ramos, 2019. "Sugarcane Bagasse Hydrolysis Enhancement by Microwave-Assisted Sulfolane Pretreatment," Energies, MDPI, vol. 12(9), pages 1-15, May.

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