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Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate

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  • Dias, Bruna
  • Lopes, Marlene
  • Fernandes, Helena
  • Marques, Susana
  • Gírio, Francisco
  • Belo, Isabel

Abstract

Using lignocellulosic biomass hydrolysate as a renewable and abundant feedstock for microbial lipids production is a sustainable and economic high-potential approach. This study investigated the potential of the oleaginous yeast Yarrowia lipolytica to produce lipids-rich biomass from eucalyptus bark hydrolysate (EBH) obtained by enzymatic hydrolysis of the biomass pretreated by steam explosion. The effect of EBH concentration (undiluted and 1:3 v/v diluted) and medium supplementation (CSL and KH2PO4) was evaluated in Erlenmeyer flasks and lab-scale stirred tank bioreactor, respectively. Additionally, the effect of volumetric oxygen transfer coefficient (kLa) and mode of operation (batch and two-stage repeated batch) was also assessed in the bioreactor. Under the best experimental conditions (undiluted EBH, 2 g⋅L−1 CSL, 1.8 g⋅L−1 (NH4)2SO4, and kLa of 66 h−1), Y. lipolytica W29 grown in batch cultures accumulated 26 % (w/w) of intracellular lipids, corresponding to 5.6 g⋅L−1 of concentration. Lipids of Y. lipolytica were highly unsaturated and mainly composed of oleic acid (48 %), followed by palmitoleic (20 %), linoleic (17 %) and palmitic acids (14 %). This composition of Y. lipolytica lipids suggests their potential use as feedstock for biodiesel (a renewable biofuel). This work demonstrated the robust features of Y. lipolytica W29 as a potential lipids production platform to implement lignocellulose-based biorefineries.

Suggested Citation

  • Dias, Bruna & Lopes, Marlene & Fernandes, Helena & Marques, Susana & Gírio, Francisco & Belo, Isabel, 2024. "Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate," Renewable Energy, Elsevier, vol. 224(C).
    • Handle: RePEc:eee:renene:v:224:y:2024:i:c:s0960148124002386
    DOI: 10.1016/j.renene.2024.120173
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    References listed on IDEAS

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    1. Pereira, Ana S. & Lopes, Marlene & Duarte, M. Salomé & Alves, M. Madalena & Belo, Isabel, 2023. "Integrated bioprocess of microbial lipids production in Yarrowia lipolytica using food-waste derived volatile fatty acids," Renewable Energy, Elsevier, vol. 202(C), pages 1470-1478.
    2. Miao, Zhengang & Tian, Xuemei & Liang, Wenxing & He, Yawen & Wang, Guangyuan, 2020. "Bioconversion of corncob hydrolysate into microbial lipid by an oleaginous yeast Rhodotorula taiwanensis AM2352 for biodiesel production," Renewable Energy, Elsevier, vol. 161(C), pages 91-97.
    3. Farias, Josiane Pinheiro & Okeke, Benedict C. & Ávila, Fernanda Dias De & Demarco, Carolina Faccio & Silva, Márcio Santos & Camargo, Flávio Anastácio de Oliveira & Menezes Bento, Fátima & Pieniz, Simo, 2023. "Biotechnology process for microbial lipid synthesis from enzymatic hydrolysate of pre-treated sugarcane bagasse for potential bio-oil production," Renewable Energy, Elsevier, vol. 205(C), pages 174-184.
    4. Liu, Zihe & Moradi, Hamideh & Shi, Shuobo & Darvishi, Farshad, 2021. "Yeasts as microbial cell factories for sustainable production of biofuels," Renewable and Sustainable Energy Reviews, Elsevier, vol. 143(C).
    5. Katarzyna Drzymała & Aleksandra Maria Mirończuk & Witold Pietrzak & Adam Dobrowolski, 2020. "Rye and Oat Agricultural Wastes as Substrate Candidates for Biomass Production of the Non-Conventional Yeast Yarrowia lipolytica," Sustainability, MDPI, vol. 12(18), pages 1-12, September.
    6. Helberth Júnnior Santos Lopes & Nemailla Bonturi & Everson Alves Miranda, 2020. "Rhodotorula toruloides Single Cell Oil Production Using Eucalyptus urograndis Hemicellulose Hydrolysate as a Carbon Source," Energies, MDPI, vol. 13(4), pages 1-11, February.
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