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Rhodotorula toruloides Single Cell Oil Production Using Eucalyptus urograndis Hemicellulose Hydrolysate as a Carbon Source

Author

Listed:
  • Helberth Júnnior Santos Lopes

    (Department of Materials and Bioprocess Engineering, School of Chemical Engineering, State University of Campinas, Campinas SP 13083-852, Brazil)

  • Nemailla Bonturi

    (Institute of Technology, University of Tartu, 50411 Tartu, Estonia)

  • Everson Alves Miranda

    (Department of Materials and Bioprocess Engineering, School of Chemical Engineering, State University of Campinas, Campinas SP 13083-852, Brazil)

Abstract

Microbial oil is a potential substitute for vegetable oils in the biodiesel industry. Efforts to obtain cheap carbon sources for the cultivation of lipid-producing microorganisms comprise an active research area. This work aimed to extract the hemicellulose fraction from Eucalyptus uograndis and to use its hydrolysate as a carbon source for Rhodotorula toruloides (an oleaginous yeast) cultivation for microbial oil production. Hemicellulose hydrothermal extractions were performed at different temperatures, times, and ratios of solid to liquid (S/L). Temperature and time showed a stronger effect on the solubilization of hemicellulose. Hemicellulose extraction at 155 °C, 195 min, and an S/L ratio of 1/2 resulted in a hydrolysate with a xylose content of 37.0 g/l. R. toruloides cultivation in this hydrolysate showed that initial pH had a strong influence on cell growth. At an initial pH of 6.2, cells grew to 6.0 g/l of biomass with a lipid content of 50%. Therefore, we believe that E. urograndis hemicellulose hydrolysate could be a potential substrate for R. toruloides for lipid production based on the biorefinery concept.

Suggested Citation

  • Helberth Júnnior Santos Lopes & Nemailla Bonturi & Everson Alves Miranda, 2020. "Rhodotorula toruloides Single Cell Oil Production Using Eucalyptus urograndis Hemicellulose Hydrolysate as a Carbon Source," Energies, MDPI, vol. 13(4), pages 1-11, February.
    • Handle: RePEc:gam:jeners:v:13:y:2020:i:4:p:795-:d:319511
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    References listed on IDEAS

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    1. Silva, F.T.M. & Ataíde, C.H., 2019. "Valorization of eucalyptus urograndis wood via carbonization: Product yields and characterization," Energy, Elsevier, vol. 172(C), pages 509-516.
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    Cited by:

    1. Li Ji & Pengfei Li & Fuhou Lei & Xianliang Song & Jianxin Jiang & Kun Wang, 2020. "Coproduction of Furfural, Phenolated Lignin and Fermentable Sugars from Bamboo with One-Pot Fractionation Using Phenol-Acidic 1,4-Dioxane," Energies, MDPI, vol. 13(20), pages 1-17, October.
    2. Erminta Tsouko & Eirini Tolia & Dimitris Sarris, 2023. "Microbial Melanin: Renewable Feedstock and Emerging Applications in Food-Related Systems," Sustainability, MDPI, vol. 15(9), pages 1-20, May.
    3. Dias, Bruna & Lopes, Marlene & Fernandes, Helena & Marques, Susana & Gírio, Francisco & Belo, Isabel, 2024. "Biomass and microbial lipids production by Yarrowia lipolytica W29 from eucalyptus bark hydrolysate," Renewable Energy, Elsevier, vol. 224(C).

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