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Fermentative hydrogen and methane production from microalgal biomass (Chlorella vulgaris) in a two-stage combined process

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  • Wieczorek, Nils
  • Kucuker, Mehmet Ali
  • Kuchta, Kerstin

Abstract

This study investigated the hydrogen fermentation in a two-stage combined fermentation process (combination of dark fermentation for hydrogen (H2) production from microalgae and methane (CH4) fermentation from residues following H2 production process). Microalgae Chlorella vulgaris culture was used as a substrate. The hydrogen production from C. vulgaris ranges 1.75±1.50–19±2.94ml H2 g-VS−1 at different substrate dosages without enzymatic pre-treatment. A seven-fold increase in H2 production yields (19±2.94–135±3.11ml H2 g-VS−1) was observed with enzymatic pre-treatment (Onozuka R-10 and Macerozyme R-10) of the microalgae. The results of the CH4 fermentation show that the methane yields could be increased from 245±2.46 to 414±2.45ml CH4 g-VS−1 by using enzymatic pretreatment. In addition, the yield of the CH4 fermentation (14.86kJ/g-VS) has approximately the same order of magnitude in comparison to the two-stage combined fermentation process (14.46kJ/g-VS) in terms of energy production.

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  • Wieczorek, Nils & Kucuker, Mehmet Ali & Kuchta, Kerstin, 2014. "Fermentative hydrogen and methane production from microalgal biomass (Chlorella vulgaris) in a two-stage combined process," Applied Energy, Elsevier, vol. 132(C), pages 108-117.
    • Handle: RePEc:eee:appene:v:132:y:2014:i:c:p:108-117
    DOI: 10.1016/j.apenergy.2014.07.003
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    5. Bakonyi, Péter & Buitrón, Germán & Valdez-Vazquez, Idania & Nemestóthy, Nándor & Bélafi-Bakó, Katalin, 2017. "A novel gas separation integrated membrane bioreactor to evaluate the impact of self-generated biogas recycling on continuous hydrogen fermentation," Applied Energy, Elsevier, vol. 190(C), pages 813-823.
    6. Merrylin Jayaseelan & Mohamed Usman & Adishkumar Somanathan & Sivashanmugam Palani & Gunasekaran Muniappan & Rajesh Banu Jeyakumar, 2021. "Microalgal Production of Biofuels Integrated with Wastewater Treatment," Sustainability, MDPI, vol. 13(16), pages 1-13, August.
    7. Tabatabaei, Meisam & Aghbashlo, Mortaza & Valijanian, Elena & Kazemi Shariat Panahi, Hamed & Nizami, Abdul-Sattar & Ghanavati, Hossein & Sulaiman, Alawi & Mirmohamadsadeghi, Safoora & Karimi, Keikhosr, 2020. "A comprehensive review on recent biological innovations to improve biogas production, Part 1: Upstream strategies," Renewable Energy, Elsevier, vol. 146(C), pages 1204-1220.
    8. Ramprakash, Balasubramani & Lindblad, Peter & Eaton-Rye, Julian J. & Incharoensakdi, Aran, 2022. "Current strategies and future perspectives in biological hydrogen production: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 168(C).
    9. Jacob, Amita & Xia, Ao & Murphy, Jerry D., 2015. "A perspective on gaseous biofuel production from micro-algae generated from CO2 from a coal-fired power plant," Applied Energy, Elsevier, vol. 148(C), pages 396-402.
    10. Bohutskyi, Pavlo & Chow, Steven & Ketter, Ben & Betenbaugh, Michael J. & Bouwer, Edward J., 2015. "Prospects for methane production and nutrient recycling from lipid extracted residues and whole Nannochloropsis salina using anaerobic digestion," Applied Energy, Elsevier, vol. 154(C), pages 718-731.
    11. Pires, José C.M., 2017. "COP21: The algae opportunity?," Renewable and Sustainable Energy Reviews, Elsevier, vol. 79(C), pages 867-877.
    12. Gao, Zhenghui & Alshehri, Khaled & Li, Yuan & Qian, Hang & Sapsford, Devin & Cleall, Peter & Harbottle, Michael, 2022. "Advances in biological techniques for sustainable lignocellulosic waste utilization in biogas production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 170(C).
    13. Ghimire, Anish & Frunzo, Luigi & Pirozzi, Francesco & Trably, Eric & Escudie, Renaud & Lens, Piet N.L. & Esposito, Giovanni, 2015. "A review on dark fermentative biohydrogen production from organic biomass: Process parameters and use of by-products," Applied Energy, Elsevier, vol. 144(C), pages 73-95.
    14. Tran Thi Giang & Siriporn Lunprom & Qiang Liao & Alissara Reungsang & Apilak Salakkam, 2019. "Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)," Energies, MDPI, vol. 12(5), pages 1-14, March.
    15. Nazarpour, Mehrshad & Taghizadeh-Alisaraei, Ahmad & Asghari, Ali & Abbaszadeh-Mayvan, Ahmad & Tatari, Aliasghar, 2022. "Optimization of biohydrogen production from microalgae by response surface methodology (RSM)," Energy, Elsevier, vol. 253(C).
    16. Xia, Ao & Cheng, Jun & Ding, Lingkan & Lin, Richen & Song, Wenlu & Su, Huibo & Zhou, Junhu & Cen, Kefa, 2015. "Substrate consumption and hydrogen production via co-fermentation of monomers derived from carbohydrates and proteins in biomass wastes," Applied Energy, Elsevier, vol. 139(C), pages 9-16.

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