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Optimization of Batch Dark Fermentation of Chlorella sp. Using Mixed-Cultures for Simultaneous Hydrogen and Butyric Acid Production

Author

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  • Nikannapas Usmanbaha

    (Energy Technology Program, Faculty of Engineering, Prince of Songkla University, Songkhla 90110, Thailand
    Bio-Mass Conversion to Energy and Chemicals (Bio-MEC) Research Unit, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand)

  • Rattana Jariyaboon

    (Bio-Mass Conversion to Energy and Chemicals (Bio-MEC) Research Unit, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand
    Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand)

  • Alissara Reungsang

    (Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, Thailand
    Research Group for Development of Microbial Hydrogen Production Process from Biomass, Khon Kaen University, Khon Kaen 40002, Thailand)

  • Prawit Kongjan

    (Bio-Mass Conversion to Energy and Chemicals (Bio-MEC) Research Unit, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand
    Department of Science, Faculty of Science and Technology, Prince of Songkla University, Pattani 94000, Thailand)

  • Chen-Yeon Chu

    (Master’s Program of Green Energy Science and Technology, Feng Chia University, Taichung 40724, Taiwan
    Green Energy Development Center, Feng Chia University, Taichung 40724, Taiwan)

Abstract

This paper reports on the optimum conditions for simultaneous hydrogen and butyric acid production from microalgae ( Chlorella sp.) using enriched anaerobic mixed cultures as inoculum. The fermentation was objectively carried out under acidogenic conditions to achieve butyric acid for further ABE fermentation in solventogenesis stage. The main effects of initial pH (5 and 7), temperature (35 °C and 55 °C), and substrate concentration (40, 60, 80, and 100 g-VS/L) for hydrogen and butyric acid production were evaluated by using batch fermentation experiment. The major effects on hydrogen and butyric acid production are pH and temperature. The highest production of hydrogen and butyric acid was observed at pH 7 and temperature 35 °C. Using initial Chlorella sp. concentration of 80 g-VS/L or 100 g-VS/L at pH 7 and temperature 35 °C could produce hydrogen with an average yield of 22 mL-H 2 /g-VS along with high butyric acid production yield of 0.05 g/g-VS, suggesting that microalgae ( Chlorella sp.) has potential to be converted directly to butyric acid by using acidogenesis under above optimum conditions.

Suggested Citation

  • Nikannapas Usmanbaha & Rattana Jariyaboon & Alissara Reungsang & Prawit Kongjan & Chen-Yeon Chu, 2019. "Optimization of Batch Dark Fermentation of Chlorella sp. Using Mixed-Cultures for Simultaneous Hydrogen and Butyric Acid Production," Energies, MDPI, vol. 12(13), pages 1-14, July.
    • Handle: RePEc:gam:jeners:v:12:y:2019:i:13:p:2529-:d:244634
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    References listed on IDEAS

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    1. Tran Thi Giang & Siriporn Lunprom & Qiang Liao & Alissara Reungsang & Apilak Salakkam, 2019. "Enhancing Hydrogen Production from Chlorella sp. Biomass by Pre-Hydrolysis with Simultaneous Saccharification and Fermentation (PSSF)," Energies, MDPI, vol. 12(5), pages 1-14, March.
    2. Wieczorek, Nils & Kucuker, Mehmet Ali & Kuchta, Kerstin, 2014. "Fermentative hydrogen and methane production from microalgal biomass (Chlorella vulgaris) in a two-stage combined process," Applied Energy, Elsevier, vol. 132(C), pages 108-117.
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    2. Joanna Kazimierowicz & Marcin Dębowski & Marcin Zieliński, 2022. "Progress and Challenges in Biohydrogen Production," Energies, MDPI, vol. 15(15), pages 1-3, July.
    3. Sekoai, Patrick T. & Ghimire, Anish & Ezeokoli, Obinna T. & Rao, Subramanya & Ngan, Wing Y. & Habimana, Olivier & Yao, Yuan & Yang, Pu & Yiu Fung, Aster Hei & Yoro, Kelvin O. & Daramola, Michael O. & , 2021. "Valorization of volatile fatty acids from the dark fermentation waste Streams-A promising pathway for a biorefinery concept," Renewable and Sustainable Energy Reviews, Elsevier, vol. 143(C).

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