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Structural analysis of the essential self-cleaving type III secretion proteins EscU and SpaS

Author

Listed:
  • Raz Zarivach

    (and the Center for Blood Research, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada)

  • Wanyin Deng

    (Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, British Columbia V6T 1Z4, Canada)

  • Marija Vuckovic

    (and the Center for Blood Research, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada)

  • Heather B. Felise

    (HSB K-140, Box 357710, Seattle, Washington 98195, USA)

  • Hai V. Nguyen

    (HSB K-140, Box 357710, Seattle, Washington 98195, USA)

  • Samuel I. Miller

    (HSB K-140, Box 357710, Seattle, Washington 98195, USA)

  • B. Brett Finlay

    (Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver, British Columbia V6T 1Z4, Canada)

  • Natalie C. J. Strynadka

    (and the Center for Blood Research, University of British Columbia, 2350 Health Sciences Mall, Vancouver, British Columbia V6T 1Z3, Canada)

Abstract

A type II β-turn in the Escherichia coli protein EscU undergoes auto-cleavage via a mechanism involving cyclization of a conserved asparagine residue. Structural and in vivo analysis of point and deletion mutations illustrates the subtle conformational effects of auto-cleavage in modulating the molecular features of a highly conserved surface region of EscU, a potential point of interaction with other T3SS components at the inner membrane.

Suggested Citation

  • Raz Zarivach & Wanyin Deng & Marija Vuckovic & Heather B. Felise & Hai V. Nguyen & Samuel I. Miller & B. Brett Finlay & Natalie C. J. Strynadka, 2008. "Structural analysis of the essential self-cleaving type III secretion proteins EscU and SpaS," Nature, Nature, vol. 453(7191), pages 124-127, May.
    • Handle: RePEc:nat:nature:v:453:y:2008:i:7191:d:10.1038_nature06832
    DOI: 10.1038/nature06832
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    Cited by:

    1. William R Taylor & Teige R S Matthews-Palmer & Morgan Beeby, 2016. "Molecular Models for the Core Components of the Flagellar Type-III Secretion Complex," PLOS ONE, Public Library of Science, vol. 11(11), pages 1-33, November.

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