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Inflammatory and Repair Pathways Induced in Human Bronchoalveolar Lavage Cells with Ozone Inhalation

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  • Pascale Leroy
  • Andrea Tham
  • Hofer Wong
  • Rachel Tenney
  • Chun Chen
  • Rachel Stiner
  • John R Balmes
  • Agnès C Paquet
  • Mehrdad Arjomandi

Abstract

Background: Inhalation of ambient levels of ozone causes airway inflammation and epithelial injury. Methods: To examine the responses of airway cells to ozone-induced oxidative injury, 19 subjects (7 with asthma) were exposed to clean air (0ppb), medium (100ppb), and high (200ppb) ambient levels of ozone for 4h on three separate occasions in a climate-controlled chamber followed by bronchoscopy with bronchoalveolar lavage (BAL) 24h later. BAL cell mRNA expression was examined using Affymetrix GeneChip Microarray. The role of a differentially expressed gene (DEG) in epithelial injury was evaluated in an in vitro model of injury [16HBE14o- cell line scratch assay]. Results: Ozone exposure caused a dose-dependent up-regulation of several biologic pathways involved in inflammation and repair including chemokine and cytokine secretion, activity, and receptor binding; metalloproteinase and endopeptidase activity; adhesion, locomotion, and migration; and cell growth and tumorigenesis regulation. Asthmatic subjects had 1.7- to 3.8-fold higher expression of many DEGs suggestive of increased proinflammatory and matrix degradation and remodeling signals. The most highly up-regulated gene was osteopontin, the protein level of which in BAL fluid increased in a dose-dependent manner after ozone exposure. Asthmatic subjects had a disproportionate increase in non-polymerized osteopontin with increasing exposure to ozone. Treatment with polymeric, but not monomeric, osteopontin enhanced the migration of epithelial cells and wound closure in an α9β1 integrin-dependent manner. Conclusions: Expression profiling of BAL cells after ozone exposure reveals potential regulatory genes and pathways activated by oxidative stress. One DEG, osteopontin, promotes epithelial wound healing in an in vitro model of injury.

Suggested Citation

  • Pascale Leroy & Andrea Tham & Hofer Wong & Rachel Tenney & Chun Chen & Rachel Stiner & John R Balmes & Agnès C Paquet & Mehrdad Arjomandi, 2015. "Inflammatory and Repair Pathways Induced in Human Bronchoalveolar Lavage Cells with Ozone Inhalation," PLOS ONE, Public Library of Science, vol. 10(6), pages 1-28, June.
    • Handle: RePEc:plo:pone00:0127283
    DOI: 10.1371/journal.pone.0127283
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    1. Margaret Sullivan Pepe & Gary Longton & Garnet L. Anderson & Michel Schummer, 2003. "Selecting Differentially Expressed Genes from Microarray Experiments," Biometrics, The International Biometric Society, vol. 59(1), pages 133-142, March.
    2. Reinhard E. Voll & Martin Herrmann & Edith A. Roth & Christian Stach & Joachim R. Kalden & Irute Girkontaite, 1997. "Immunosuppressive effects of apoptotic cells," Nature, Nature, vol. 390(6658), pages 350-351, November.
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    1. Izabela Barczyk & Diana Masłyk & Natalia Walczuk & Karina Kijak & Piotr Skomro & Helena Gronwald & Maria Pawlak & Angelika Rusińska & Natalia Sadowska & Barbara Gronwald & Adam Andrzej Garstka & Danut, 2023. "Potential Clinical Applications of Ozone Therapy in Dental Specialties—A Literature Review, Supported by Own Observations," IJERPH, MDPI, vol. 20(3), pages 1-15, January.

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