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Residual Structures, Conformational Fluctuations, and Electrostatic Interactions in the Synergistic Folding of Two Intrinsically Disordered Proteins

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  • Weihong Zhang
  • Debabani Ganguly
  • Jianhan Chen

Abstract

To understand the interplay of residual structures and conformational fluctuations in the interaction of intrinsically disordered proteins (IDPs), we first combined implicit solvent and replica exchange sampling to calculate atomistic disordered ensembles of the nuclear co-activator binding domain (NCBD) of transcription coactivator CBP and the activation domain of the p160 steroid receptor coactivator ACTR. The calculated ensembles are in quantitative agreement with NMR-derived residue helicity and recapitulate the experimental observation that, while free ACTR largely lacks residual secondary structures, free NCBD is a molten globule with a helical content similar to that in the folded complex. Detailed conformational analysis reveals that free NCBD has an inherent ability to substantially sample all the helix configurations that have been previously observed either unbound or in complexes. Intriguingly, further high-temperature unbinding and unfolding simulations in implicit and explicit solvents emphasize the importance of conformational fluctuations in synergistic folding of NCBD with ACTR. A balance between preformed elements and conformational fluctuations appears necessary to allow NCBD to interact with different targets and fold into alternative conformations. Together with previous topology-based modeling and existing experimental data, the current simulations strongly support an “extended conformational selection” synergistic folding mechanism that involves a key intermediate state stabilized by interaction between the C-terminal helices of NCBD and ACTR. In addition, the atomistic simulations reveal the role of long-range as well as short-range electrostatic interactions in cooperating with readily fluctuating residual structures, which might enhance the encounter rate and promote efficient folding upon encounter for facile binding and folding interactions of IDPs. Thus, the current study not only provides a consistent mechanistic understanding of the NCBD/ACTR interaction, but also helps establish a multi-scale molecular modeling framework for understanding the structure, interaction, and regulation of IDPs in general. Author Summary: Intrinsically disordered proteins (IDPs) are now widely recognized to play fundamental roles in biology and to be frequently associated with human diseases. Although the potential advantages of intrinsic disorder in cellular signaling and regulation have been widely discussed, the physical basis for these proposed phenomena remains sketchy at best. An integration of multi-scale molecular modeling and experimental characterization is necessary to uncover the molecular principles that govern the structure, interaction, and regulation of IDPs. In this work, we characterize the conformational properties of two IDPs involved in transcription regulation at the atomistic level and further examine the roles of these properties in their coupled binding and folding interactions. Our simulations suggest interplay among residual structures, conformational fluctuations, and electrostatic interactions that allows efficient synergistic folding of these two IDPs. In particular, we propose that electrostatic interactions might play an important role in facilitating rapid folding and binding recognition of IDPs, by enhancing the encounter rate and promoting efficient folding upon encounter.

Suggested Citation

  • Weihong Zhang & Debabani Ganguly & Jianhan Chen, 2012. "Residual Structures, Conformational Fluctuations, and Electrostatic Interactions in the Synergistic Folding of Two Intrinsically Disordered Proteins," PLOS Computational Biology, Public Library of Science, vol. 8(1), pages 1-15, January.
  • Handle: RePEc:plo:pcbi00:1002353
    DOI: 10.1371/journal.pcbi.1002353
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    References listed on IDEAS

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    1. Stephen J. Demarest & Maria Martinez-Yamout & John Chung & Hongwu Chen & Wei Xu & H. Jane Dyson & Ronald M. Evans & Peter E. Wright, 2002. "Mutual synergistic folding in recruitment of CBP/p300 by p160 nuclear receptor coactivators," Nature, Nature, vol. 415(6871), pages 549-553, January.
    2. Christopher M. Dobson, 2003. "Protein folding and misfolding," Nature, Nature, vol. 426(6968), pages 884-890, December.
    3. Kenji Sugase & H. Jane Dyson & Peter E. Wright, 2007. "Mechanism of coupled folding and binding of an intrinsically disordered protein," Nature, Nature, vol. 447(7147), pages 1021-1025, June.
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    1. Debabani Ganguly & Weihong Zhang & Jianhan Chen, 2013. "Electrostatically Accelerated Encounter and Folding for Facile Recognition of Intrinsically Disordered Proteins," PLOS Computational Biology, Public Library of Science, vol. 9(11), pages 1-12, November.
    2. Fan Jin & Chen Yu & Luhua Lai & Zhirong Liu, 2013. "Ligand Clouds around Protein Clouds: A Scenario of Ligand Binding with Intrinsically Disordered Proteins," PLOS Computational Biology, Public Library of Science, vol. 9(10), pages 1-11, October.

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