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SiCTeC: An inexpensive, easily assembled Peltier device for rapid temperature shifting during single-cell imaging

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  • Benjamin D Knapp
  • Lillian Zhu
  • Kerwyn Casey Huang

Abstract

Single-cell imaging, combined with recent advances in image analysis and microfluidic technologies, have enabled fundamental discoveries of cellular responses to chemical perturbations that are often obscured by traditional liquid-culture experiments. Temperature is an environmental variable well known to impact growth and to elicit specific stress responses at extreme values; it is often used as a genetic tool to interrogate essential genes. However, the dynamic effects of temperature shifts have remained mostly unstudied at the single-cell level, due largely to engineering challenges related to sample stability, heatsink considerations, and temperature measurement and feedback. Additionally, the few commercially available temperature-control platforms are costly. Here, we report an inexpensive (

Suggested Citation

  • Benjamin D Knapp & Lillian Zhu & Kerwyn Casey Huang, 2020. "SiCTeC: An inexpensive, easily assembled Peltier device for rapid temperature shifting during single-cell imaging," PLOS Biology, Public Library of Science, vol. 18(11), pages 1-17, November.
    • Handle: RePEc:plo:pbio00:3000786
    DOI: 10.1371/journal.pbio.3000786
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    References listed on IDEAS

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    1. Enrique R. Rojas & Gabriel Billings & Pascal D. Odermatt & George K. Auer & Lillian Zhu & Amanda Miguel & Fred Chang & Douglas B. Weibel & Julie A. Theriot & Kerwyn Casey Huang, 2018. "The outer membrane is an essential load-bearing element in Gram-negative bacteria," Nature, Nature, vol. 559(7715), pages 617-621, July.
    2. André Maia Chagas, 2018. "Haves and have nots must find a better way: The case for open scientific hardware," PLOS Biology, Public Library of Science, vol. 16(9), pages 1-8, September.
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