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Single-shot Ad26 vaccine protects against SARS-CoV-2 in rhesus macaques

Author

Listed:
  • Noe B. Mercado

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Roland Zahn

    (Janssen Vaccines and Prevention BV)

  • Frank Wegmann

    (Janssen Vaccines and Prevention BV)

  • Carolin Loos

    (Ragon Institute of MGH, MIT and Harvard
    Massachusetts Institute of Technology)

  • Abishek Chandrashekar

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Jingyou Yu

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Jinyan Liu

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Lauren Peter

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Katherine McMahan

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Lisa H. Tostanoski

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Xuan He

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • David R. Martinez

    (University of North Carolina at Chapel Hill)

  • Lucy Rutten

    (Janssen Vaccines and Prevention BV)

  • Rinke Bos

    (Janssen Vaccines and Prevention BV)

  • Danielle Manen

    (Janssen Vaccines and Prevention BV)

  • Jort Vellinga

    (Janssen Vaccines and Prevention BV)

  • Jerome Custers

    (Janssen Vaccines and Prevention BV)

  • Johannes P. Langedijk

    (Janssen Vaccines and Prevention BV)

  • Ted Kwaks

    (Janssen Vaccines and Prevention BV)

  • Mark J. G. Bakkers

    (Janssen Vaccines and Prevention BV)

  • David Zuijdgeest

    (Janssen Vaccines and Prevention BV)

  • Sietske K. Rosendahl Huber

    (Janssen Vaccines and Prevention BV)

  • Caroline Atyeo

    (Ragon Institute of MGH, MIT and Harvard
    Harvard Medical School)

  • Stephanie Fischinger

    (Ragon Institute of MGH, MIT and Harvard
    Harvard Medical School)

  • John S. Burke

    (Ragon Institute of MGH, MIT and Harvard)

  • Jared Feldman

    (Ragon Institute of MGH, MIT and Harvard
    Harvard Medical School)

  • Blake M. Hauser

    (Ragon Institute of MGH, MIT and Harvard
    Harvard Medical School)

  • Timothy M. Caradonna

    (Ragon Institute of MGH, MIT and Harvard
    Harvard Medical School)

  • Esther A. Bondzie

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Gabriel Dagotto

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School
    Harvard Medical School)

  • Makda S. Gebre

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School
    Harvard Medical School)

  • Emily Hoffman

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Catherine Jacob-Dolan

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School
    Harvard Medical School)

  • Marinela Kirilova

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Zhenfeng Li

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Zijin Lin

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Shant H. Mahrokhian

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Lori F. Maxfield

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Felix Nampanya

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Ramya Nityanandam

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Joseph P. Nkolola

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Shivani Patel

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • John D. Ventura

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Kaylee Verrington

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Huahua Wan

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Laurent Pessaint

    (Bioqual)

  • Alex Ry

    (Bioqual)

  • Kelvin Blade

    (Bioqual)

  • Amanda Strasbaugh

    (Bioqual)

  • Mehtap Cabus

    (Bioqual)

  • Renita Brown

    (Bioqual)

  • Anthony Cook

    (Bioqual)

  • Serge Zouantchangadou

    (Bioqual)

  • Elyse Teow

    (Bioqual)

  • Hanne Andersen

    (Bioqual)

  • Mark G. Lewis

    (Bioqual)

  • Yongfei Cai

    (Children’s Hospital)

  • Bing Chen

    (Children’s Hospital
    Massachusetts Consortium on Pathogen Readiness)

  • Aaron G. Schmidt

    (Ragon Institute of MGH, MIT and Harvard
    Harvard Medical School
    Massachusetts Consortium on Pathogen Readiness)

  • R. Keith Reeves

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School)

  • Ralph S. Baric

    (University of North Carolina at Chapel Hill)

  • Douglas A. Lauffenburger

    (Massachusetts Institute of Technology)

  • Galit Alter

    (Ragon Institute of MGH, MIT and Harvard
    Massachusetts Consortium on Pathogen Readiness)

  • Paul Stoffels

    (Janssen Vaccines and Prevention BV)

  • Mathai Mammen

    (Janssen Vaccines and Prevention BV)

  • Johan Hoof

    (Janssen Vaccines and Prevention BV)

  • Hanneke Schuitemaker

    (Janssen Vaccines and Prevention BV)

  • Dan H. Barouch

    (Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School
    Ragon Institute of MGH, MIT and Harvard
    Harvard Medical School
    Massachusetts Consortium on Pathogen Readiness)

Abstract

A safe and effective vaccine for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) may be required to end the coronavirus disease 2019 (COVID-19) pandemic1–8. For global deployment and pandemic control, a vaccine that requires only a single immunization would be optimal. Here we show the immunogenicity and protective efficacy of a single dose of adenovirus serotype 26 (Ad26) vector-based vaccines expressing the SARS-CoV-2 spike (S) protein in non-human primates. Fifty-two rhesus macaques (Macaca mulatta) were immunized with Ad26 vectors that encoded S variants or sham control, and then challenged with SARS-CoV-2 by the intranasal and intratracheal routes9,10. The optimal Ad26 vaccine induced robust neutralizing antibody responses and provided complete or near-complete protection in bronchoalveolar lavage and nasal swabs after SARS-CoV-2 challenge. Titres of vaccine-elicited neutralizing antibodies correlated with protective efficacy, suggesting an immune correlate of protection. These data demonstrate robust single-shot vaccine protection against SARS-CoV-2 in non-human primates. The optimal Ad26 vector-based vaccine for SARS-CoV-2, termed Ad26.COV2.S, is currently being evaluated in clinical trials.

Suggested Citation

  • Noe B. Mercado & Roland Zahn & Frank Wegmann & Carolin Loos & Abishek Chandrashekar & Jingyou Yu & Jinyan Liu & Lauren Peter & Katherine McMahan & Lisa H. Tostanoski & Xuan He & David R. Martinez & Lu, 2020. "Single-shot Ad26 vaccine protects against SARS-CoV-2 in rhesus macaques," Nature, Nature, vol. 586(7830), pages 583-588, October.
    • Handle: RePEc:nat:nature:v:586:y:2020:i:7830:d:10.1038_s41586-020-2607-z
    DOI: 10.1038/s41586-020-2607-z
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    Citations

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    Cited by:

    1. Ramon Roozendaal & Laura Solforosi & Daniel J. Stieh & Jan Serroyen & Roel Straetemans & Anna Dari & Muriel Boulton & Frank Wegmann & Sietske K. Rosendahl Huber & Joan E. M. van der Lubbe & Jenny Hend, 2021. "SARS-CoV-2 binding and neutralizing antibody levels after Ad26.COV2.S vaccination predict durable protection in rhesus macaques," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    2. Amy R. Rappaport & Sue-Jean Hong & Ciaran D. Scallan & Leonid Gitlin & Arvin Akoopie & Gregory R. Boucher & Milana Egorova & J. Aaron Espinosa & Mario Fidanza & Melissa A. Kachura & Annie Shen & Glori, 2022. "Low-dose self-amplifying mRNA COVID-19 vaccine drives strong protective immunity in non-human primates against SARS-CoV-2 infection," Nature Communications, Nature, vol. 13(1), pages 1-10, December.
    3. Neeltje van Doremalen & Jonathan E. Schulz & Danielle R. Adney & Taylor A. Saturday & Robert J. Fischer & Claude Kwe Yinda & Nazia Thakur & Joseph Newman & Marta Ulaszewska & Sandra Belij-Rammerstorfe, 2022. "ChAdOx1 nCoV-19 (AZD1222) or nCoV-19-Beta (AZD2816) protect Syrian hamsters against Beta Delta and Omicron variants," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    4. Jaclyn A. Kaiser & Christine E. Nelson & Xueqiao Liu & Hong-Su Park & Yumiko Matsuoka & Cindy Luongo & Celia Santos & Laura R. H. Ahlers & Richard Herbert & Ian N. Moore & Temeri Wilder-Kofie & Rashid, 2024. "Mucosal prime-boost immunization with live murine pneumonia virus-vectored SARS-CoV-2 vaccine is protective in macaques," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    5. Ankita Leekha & Arash Saeedi & K M Samiur Rahman Sefat & Monish Kumar & Melisa Martinez-Paniagua & Adrian Damian & Rohan Kulkarni & Kate Reichel & Ali Rezvan & Shalaleh Masoumi & Xinli Liu & Laurence , 2024. "Multi-antigen intranasal vaccine protects against challenge with sarbecoviruses and prevents transmission in hamsters," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    6. Laura Solforosi & Lea M. M. Costes & Jeroen T. B. M. Tolboom & Katherine McMahan & Tochi Anioke & David Hope & Tetyana Murdza & Michaela Sciacca & Emily Bouffard & Julia Barrett & Cindy Wu & Nicole Ha, 2023. "Booster with Ad26.COV2.S or Omicron-adapted vaccine enhanced immunity and efficacy against SARS-CoV-2 Omicron in macaques," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    7. Amit A. Upadhyay & Elise G. Viox & Timothy N. Hoang & Arun K. Boddapati & Maria Pino & Michelle Y.-H. Lee & Jacqueline Corry & Zachary Strongin & David A. Cowan & Elizabeth N. Beagle & Tristan R. Hort, 2023. "TREM2+ and interstitial-like macrophages orchestrate airway inflammation in SARS-CoV-2 infection in rhesus macaques," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    8. Shubhadeep Roychoudhury & Anandan Das & Pallav Sengupta & Sulagna Dutta & Shatabhisha Roychoudhury & Arun Paul Choudhury & A. B. Fuzayel Ahmed & Saumendra Bhattacharjee & Petr Slama, 2020. "Viral Pandemics of the Last Four Decades: Pathophysiology, Health Impacts and Perspectives," IJERPH, MDPI, vol. 17(24), pages 1-39, December.
    9. Juan Liu & Fengfeng Mao & Jianhe Chen & Shuaiyao Lu & Yonghe Qi & Yinyan Sun & Linqiang Fang & Man Lung Yeung & Chunmei Liu & Guimei Yu & Guangyu Li & Ximing Liu & Yuansheng Yao & Panpan Huang & Dongx, 2023. "An IgM-like inhalable ACE2 fusion protein broadly neutralizes SARS-CoV-2 variants," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    10. Chaim A. Schramm & Damee Moon & Lowrey Peyton & Noemia S. Lima & Christian Wake & Kristin L. Boswell & Amy R. Henry & Farida Laboune & David Ambrozak & Samuel W. Darko & I-Ting Teng & Kathryn E. Fould, 2023. "Interaction dynamics between innate and adaptive immune cells responding to SARS-CoV-2 vaccination in non-human primates," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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