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Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall

Author

Listed:
  • Alexandre Poulhazan

    (Université du Québec à Montréal)

  • Alexandre A. Arnold

    (Université du Québec à Montréal)

  • Frederic Mentink-Vigier

    (Florida State University)

  • Artur Muszyński

    (University of Georgia)

  • Parastoo Azadi

    (University of Georgia)

  • Adnan Halim

    (University of Copenhagen)

  • Sergey Y. Vakhrushev

    (University of Copenhagen)

  • Hiren Jitendra Joshi

    (University of Copenhagen)

  • Tuo Wang

    (Michigan State University)

  • Dror E. Warschawski

    (Sorbonne Université, École Normale Supérieure, PSL University)

  • Isabelle Marcotte

    (Université du Québec à Montréal)

Abstract

Microalgae are a renewable and promising biomass for large-scale biofuel, food and nutrient production. However, their efficient exploitation depends on our knowledge of the cell wall composition and organization as it can limit access to high-value molecules. Here we provide an atomic-level model of the non-crystalline and water-insoluble glycoprotein-rich cell wall of Chlamydomonas reinhardtii. Using in situ solid-state and sensitivity-enhanced nuclear magnetic resonance, we reveal unprecedented details on the protein and carbohydrate composition and their nanoscale heterogeneity, as well as the presence of spatially segregated protein- and glycan-rich regions with different dynamics and hydration levels. We show that mannose-rich lower-molecular-weight proteins likely contribute to the cell wall cohesion by binding to high-molecular weight protein components, and that water provides plasticity to the cell-wall architecture. The structural insight exemplifies strategies used by nature to form cell walls devoid of cellulose or other glycan polymers.

Suggested Citation

  • Alexandre Poulhazan & Alexandre A. Arnold & Frederic Mentink-Vigier & Artur Muszyński & Parastoo Azadi & Adnan Halim & Sergey Y. Vakhrushev & Hiren Jitendra Joshi & Tuo Wang & Dror E. Warschawski & Is, 2024. "Molecular-level architecture of Chlamydomonas reinhardtii’s glycoprotein-rich cell wall," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45246-7
    DOI: 10.1038/s41467-024-45246-7
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    References listed on IDEAS

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    1. Jae-Hoon Hwang & Hyun-Chul Kim & Jeong-A Choi & R.A.I. Abou-Shanab & Brian A. Dempsey & John M Regan & Jung Rae Kim & Hocheol Song & In-Hyun Nam & Su-Nam Kim & Woojung Lee & Donghee Park & Yongje Kim , 2014. "Photoautotrophic hydrogen production by eukaryotic microalgae under aerobic conditions," Nature Communications, Nature, vol. 5(1), pages 1-6, May.
    2. Oliver M. Terrett & Jan J. Lyczakowski & Li Yu & Dinu Iuga & W. Trent Franks & Steven P. Brown & Ray Dupree & Paul Dupree, 2019. "Molecular architecture of softwood revealed by solid-state NMR," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
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