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Light-dependent modulation of protein localization and function in living bacteria cells

Author

Listed:
  • Ryan McQuillen

    (The Johns Hopkins University School of Medicine)

  • Amilcar J. Perez

    (The Johns Hopkins University School of Medicine)

  • Xinxing Yang

    (The Johns Hopkins University School of Medicine)

  • Christopher H. Bohrer

    (The Johns Hopkins University School of Medicine)

  • Erika L. Smith

    (The Johns Hopkins University School of Medicine)

  • Sylvia Chareyre

    (National Institutes of Health)

  • Ho-Ching Tiffany Tsui

    (Indiana University Bloomington)

  • Kevin E. Bruce

    (Indiana University Bloomington)

  • Yin Mon Hla

    (Indiana University Bloomington)

  • Joshua W. McCausland

    (The Johns Hopkins University School of Medicine)

  • Malcolm E. Winkler

    (Indiana University Bloomington)

  • Erin D. Goley

    (The Johns Hopkins University School of Medicine)

  • Kumaran S. Ramamurthi

    (National Institutes of Health)

  • Jie Xiao

    (The Johns Hopkins University School of Medicine)

Abstract

Most bacteria lack membrane-enclosed organelles and rely on macromolecular scaffolds at different subcellular locations to recruit proteins for specific functions. Here, we demonstrate that the optogenetic CRY2-CIB1 system from Arabidopsis thaliana can be used to rapidly direct proteins to different subcellular locations with varying efficiencies in live Escherichia coli cells, including the nucleoid, the cell pole, the membrane, and the midcell division plane. Such light-induced re-localization can be used to rapidly inhibit cytokinesis in actively dividing E. coli cells. We further show that CRY2-CIBN binding kinetics can be modulated by green light, adding a new dimension of control to the system. Finally, we test this optogenetic system in three additional bacterial species, Bacillus subtilis, Caulobacter crescentus, and Streptococcus pneumoniae, providing important considerations for this system’s applicability in bacterial cell biology.

Suggested Citation

  • Ryan McQuillen & Amilcar J. Perez & Xinxing Yang & Christopher H. Bohrer & Erika L. Smith & Sylvia Chareyre & Ho-Ching Tiffany Tsui & Kevin E. Bruce & Yin Mon Hla & Joshua W. McCausland & Malcolm E. W, 2024. "Light-dependent modulation of protein localization and function in living bacteria cells," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54974-9
    DOI: 10.1038/s41467-024-54974-9
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    References listed on IDEAS

    as
    1. Fuun Kawano & Hideyuki Suzuki & Akihiro Furuya & Moritoshi Sato, 2015. "Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins," Nature Communications, Nature, vol. 6(1), pages 1-8, May.
    2. Amir Taslimi & Justin D. Vrana & Daniel Chen & Sofya Borinskaya & Bruce J. Mayer & Matthew J. Kennedy & Chandra L. Tucker, 2014. "An optimized optogenetic clustering tool for probing protein interaction and function," Nature Communications, Nature, vol. 5(1), pages 1-9, December.
    3. Sebastian M. Castillo-Hair & Elliot A. Baerman & Masaya Fujita & Oleg A. Igoshin & Jeffrey J. Tabor, 2019. "Optogenetic control of Bacillus subtilis gene expression," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    4. Joshua W. McCausland & Xinxing Yang & Georgia R. Squyres & Zhixin Lyu & Kevin E. Bruce & Melissa M. Lamanna & Bill Söderström & Ethan C. Garner & Malcolm E. Winkler & Jie Xiao & Jian Liu, 2021. "Treadmilling FtsZ polymers drive the directional movement of sPG-synthesis enzymes via a Brownian ratchet mechanism," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    5. Liting Duan & Jen Hope & Qunxiang Ong & Hsin-Ya Lou & Namdoo Kim & Comfrey McCarthy & Victor Acero & Michael Z. Lin & Bianxiao Cui, 2017. "Understanding CRY2 interactions for optical control of intracellular signaling," Nature Communications, Nature, vol. 8(1), pages 1-10, December.
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