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Assembly dynamics of FtsZ and DamX during infection-related filamentation and division in uropathogenic E. coli

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  • Bill Söderström

    (University of Technology Sydney)

  • Matthew J. Pittorino

    (University of Technology Sydney)

  • Daniel O. Daley

    (Stockholm University)

  • Iain G. Duggin

    (University of Technology Sydney)

Abstract

During infection of bladder epithelial cells, uropathogenic Escherichia coli (UPEC) can stop dividing and grow into highly filamentous forms. Here, we find that some filaments of E. coli UTI89 released from infected cells grow very rapidly and by more than 100 μm before initiating division, whereas others do not survive, suggesting that infection-related filamentation (IRF) is a stress response that promotes bacterial dispersal. IRF is accompanied by unstable, dynamic repositioning of FtsZ division rings. In contrast, DamX, which is associated with normal cell division and is also essential for IRF, is distributed uniformly around the cell envelope during filamentation. When filaments initiate division to regenerate rod cells, DamX condenses into stable rings prior to division. The DamX rings maintain consistent thickness during constriction and remain at the septum until after membrane fusion. Deletion of damX affects vegetative cell division in UTI89 (but not in the model E. coli K-12), and, during infection, blocks filamentation and reduces bacterial cell integrity. IRF therefore involves DamX distribution throughout the membrane and prevention of FtsZ ring stabilization, leading to cell division arrest. DamX then reassembles into stable division rings for filament division, promoting dispersal and survival during infection.

Suggested Citation

  • Bill Söderström & Matthew J. Pittorino & Daniel O. Daley & Iain G. Duggin, 2022. "Assembly dynamics of FtsZ and DamX during infection-related filamentation and division in uropathogenic E. coli," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31378-1
    DOI: 10.1038/s41467-022-31378-1
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    References listed on IDEAS

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    1. Marten Postma & Joachim Goedhart, 2019. "PlotsOfData—A web app for visualizing data together with their summaries," PLOS Biology, Public Library of Science, vol. 17(3), pages 1-8, March.
    2. Joshua W. McCausland & Xinxing Yang & Georgia R. Squyres & Zhixin Lyu & Kevin E. Bruce & Melissa M. Lamanna & Bill Söderström & Ethan C. Garner & Malcolm E. Winkler & Jie Xiao & Jian Liu, 2021. "Treadmilling FtsZ polymers drive the directional movement of sPG-synthesis enzymes via a Brownian ratchet mechanism," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    3. Bill Söderström & Alexander Badrutdinov & Helena Chan & Ulf Skoglund, 2018. "Cell shape-independent FtsZ dynamics in synthetically remodeled bacterial cells," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
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