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Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins

Author

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  • Fuun Kawano

    (Graduate School of Arts and Sciences, The University of Tokyo)

  • Hideyuki Suzuki

    (Graduate School of Arts and Sciences, The University of Tokyo)

  • Akihiro Furuya

    (Graduate School of Arts and Sciences, The University of Tokyo)

  • Moritoshi Sato

    (Graduate School of Arts and Sciences, The University of Tokyo)

Abstract

Optogenetic methods take advantage of photoswitches to control the activity of cellular proteins. Here, we completed a multi-directional engineering of the fungal photoreceptor Vivid to develop pairs of distinct photoswitches named Magnets. These new photoswitches were engineered to recognize each other based on the electrostatic interactions, thus preventing homodimerization and enhancing light-induced heterodimerization. Furthermore, we tuned the switch-off kinetics by four orders of magnitude and developed several variants, including those with substantially faster kinetics than any of the other conventional dimerization-based blue spectrum photoswitches. We demonstrate the utility of Magnets as powerful tools that can optogenetically manipulate molecular processes in biological systems.

Suggested Citation

  • Fuun Kawano & Hideyuki Suzuki & Akihiro Furuya & Moritoshi Sato, 2015. "Engineered pairs of distinct photoswitches for optogenetic control of cellular proteins," Nature Communications, Nature, vol. 6(1), pages 1-8, May.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms7256
    DOI: 10.1038/ncomms7256
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    Cited by:

    1. Dennis Vettkötter & Martin Schneider & Brady D. Goulden & Holger Dill & Jana Liewald & Sandra Zeiler & Julia Guldan & Yilmaz Arda Ateş & Shigeki Watanabe & Alexander Gottschalk, 2022. "Rapid and reversible optogenetic silencing of synaptic transmission by clustering of synaptic vesicles," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    2. Miguel Vizoso & Colin E. J. Pritchard & Lorenzo Bombardelli & Bram van den Broek & Paul Krimpenfort & Roderick L. Beijersbergen & Kees Jalink & Jacco van Rheenen, 2022. "A doxycycline- and light-inducible Cre recombinase mouse model for optogenetic genome editing," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    3. Deqiang Kong & Yang Zhou & Yu Wei & Xinyi Wang & Qin Huang & Xianyun Gao & Hang Wan & Mengyao Liu & Liping Kang & Guiling Yu & Jianli Yin & Ningzi Guan & Haifeng Ye, 2024. "Exploring plant-derived phytochrome chaperone proteins for light-switchable transcriptional regulation in mammals," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    4. Miaowei Mao & Yajie Qian & Wenyao Zhang & Siyu Zhou & Zefeng Wang & Xianjun Chen & Yi Yang, 2023. "Controlling protein stability with SULI, a highly sensitive tag for stabilization upon light induction," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    5. Jeonghye Yu & Jongpil Shin & Jihwan Yu & Jihye Kim & Daseuli Yu & Won Do Heo, 2024. "Programmable RNA base editing with photoactivatable CRISPR-Cas13," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    6. Charlotte A. Cialek & Gabriel Galindo & Tatsuya Morisaki & Ning Zhao & Taiowa A. Montgomery & Timothy J. Stasevich, 2022. "Imaging translational control by Argonaute with single-molecule resolution in live cells," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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