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FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli

Author

Listed:
  • Zhixin Lyu

    (Johns Hopkins School of Medicine)

  • Atsushi Yahashiri

    (University of Iowa Carver College of Medicine)

  • Xinxing Yang

    (Johns Hopkins School of Medicine
    University of Science and Technology of China)

  • Joshua W. McCausland

    (Johns Hopkins School of Medicine)

  • Gabriela M. Kaus

    (University of Iowa Carver College of Medicine)

  • Ryan McQuillen

    (Johns Hopkins School of Medicine)

  • David S. Weiss

    (University of Iowa Carver College of Medicine)

  • Jie Xiao

    (Johns Hopkins School of Medicine)

Abstract

FtsN plays an essential role in promoting the inward synthesis of septal peptidoglycan (sPG) by the FtsWI complex during bacterial cell division. How it achieves this role is unclear. Here we use single-molecule tracking to investigate FtsN’s dynamics during sPG synthesis in E. coli. We show that septal FtsN molecules move processively at ~9 nm s−1, the same as FtsWI molecules engaged in sPG synthesis (termed sPG-track), but much slower than the ~30 nm s−1 speed of inactive FtsWI molecules coupled to FtsZ’s treadmilling dynamics (termed FtsZ-track). Importantly, processive movement of FtsN is exclusively coupled to sPG synthesis and is required to maintain active sPG synthesis by FtsWI. Our findings indicate that FtsN is part of the FtsWI sPG synthesis complex, and that while FtsN is often described as a “trigger” for the initiation for cell wall constriction, it must remain part of the processive FtsWI complex to maintain sPG synthesis activity.

Suggested Citation

  • Zhixin Lyu & Atsushi Yahashiri & Xinxing Yang & Joshua W. McCausland & Gabriela M. Kaus & Ryan McQuillen & David S. Weiss & Jie Xiao, 2022. "FtsN maintains active septal cell wall synthesis by forming a processive complex with the septum-specific peptidoglycan synthases in E. coli," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33404-8
    DOI: 10.1038/s41467-022-33404-8
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    References listed on IDEAS

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    1. João M. Monteiro & Ana R. Pereira & Nathalie T. Reichmann & Bruno M. Saraiva & Pedro B. Fernandes & Helena Veiga & Andreia C. Tavares & Margarida Santos & Maria T. Ferreira & Vânia Macário & Michael S, 2018. "Peptidoglycan synthesis drives an FtsZ-treadmilling-independent step of cytokinesis," Nature, Nature, vol. 554(7693), pages 528-532, February.
    2. Joshua W. McCausland & Xinxing Yang & Georgia R. Squyres & Zhixin Lyu & Kevin E. Bruce & Melissa M. Lamanna & Bill Söderström & Ethan C. Garner & Malcolm E. Winkler & Jie Xiao & Jian Liu, 2021. "Treadmilling FtsZ polymers drive the directional movement of sPG-synthesis enzymes via a Brownian ratchet mechanism," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    3. Martín Alcorlo & David A. Dik & Stefania Benedetti & Kiran V. Mahasenan & Mijoon Lee & Teresa Domínguez-Gil & Dusan Hesek & Elena Lastochkin & Daniel López & Bill Boggess & Shahriar Mobashery & Juan A, 2019. "Structural basis of denuded glycan recognition by SPOR domains in bacterial cell division," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
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    Cited by:

    1. Brooke M. Britton & Remy A. Yovanno & Sara F. Costa & Joshua McCausland & Albert Y. Lau & Jie Xiao & Zach Hensel, 2023. "Conformational changes in the essential E. coli septal cell wall synthesis complex suggest an activation mechanism," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Han Gong & Di Yan & Yuanyuan Cui & Ying Li & Jize Yang & Wenjie Yang & Rui Zhan & Qianqian Wan & Xinci Wang & Haofeng He & Xiangdong Chen & Joe Lutkenhaus & Xinxing Yang & Shishen Du, 2024. "The divisome is a self-enhancing machine in Escherichia coli and Caulobacter crescentus," Nature Communications, Nature, vol. 15(1), pages 1-19, December.

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