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Coacervate-pore complexes for selective molecular transport and dynamic reconfiguration

Author

Listed:
  • Hao Wang

    (South China University of Technology
    South China University of Technology
    South China University of Technology)

  • Hui Zhuang

    (South China Agricultural University)

  • Wenjing Tang

    (South China University of Technology)

  • Jun Zhu

    (South China University of Technology
    South China University of Technology
    South China University of Technology)

  • Wei Zhu

    (South China University of Technology)

  • Lingxiang Jiang

    (South China University of Technology
    South China University of Technology
    South China University of Technology)

Abstract

Despite surging interests on liquid-state coacervates and condensates, confinement within solid-state pores for selective permeation remains an unexplored area. Drawing inspiration from nuclear pore complexes (NPCs), we design and construct coacervate-pore complexes (CPCs) with regulatable permeability. We demonstrate universal CPC formation across 19 coacervate systems and 5 pore types, where capillarity drives the spontaneous imbibition of coacervate droplets into dispersed or interconnected pores. CPCs regulate through-pore transport by forming a fluidic network that modulates guest molecule permeability based on guest-coacervate affinity, mimicking NPC selectivity. While solid constructs of NPC mimicries are limited by spatial fixation of polymer chains, CPCs of a liquid nature feature dynamic healing and rapid phase transitioning for permeability recovery and regulation, respectively. Looking forward, we expect the current work to establish a basis for developing liquid-based NPC analogs using a large pool of synthetic coacervates and biomolecular condensates.

Suggested Citation

  • Hao Wang & Hui Zhuang & Wenjing Tang & Jun Zhu & Wei Zhu & Lingxiang Jiang, 2024. "Coacervate-pore complexes for selective molecular transport and dynamic reconfiguration," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54510-9
    DOI: 10.1038/s41467-024-54510-9
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    1. Miao Yu & Maziar Heidari & Sofya Mikhaleva & Piau Siong Tan & Sara Mingu & Hao Ruan & Christopher D. Reinkemeier & Agnieszka Obarska-Kosinska & Marc Siggel & Martin Beck & Gerhard Hummer & Edward A. L, 2023. "Visualizing the disordered nuclear transport machinery in situ," Nature, Nature, vol. 617(7959), pages 162-169, May.
    2. Justas Svirelis & Zeynep Adali & Gustav Emilsson & Jesper Medin & John Andersson & Radhika Vattikunta & Mats Hulander & Julia Järlebark & Krzysztof Kolman & Oliver Olsson & Yusuke Sakiyama & Roderick , 2023. "Stable trapping of multiple proteins at physiological conditions using nanoscale chambers with macromolecular gates," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    3. Seung Joong Kim & Javier Fernandez-Martinez & Ilona Nudelman & Yi Shi & Wenzhu Zhang & Barak Raveh & Thurston Herricks & Brian D. Slaughter & Joanna A. Hogan & Paula Upla & Ilan E. Chemmama & Riccardo, 2018. "Integrative structure and functional anatomy of a nuclear pore complex," Nature, Nature, vol. 555(7697), pages 475-482, March.
    4. Sheung Chun Ng & Thomas Güttler & Dirk Görlich, 2021. "Recapitulation of selective nuclear import and export with a perfectly repeated 12mer GLFG peptide," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    5. Can Xu & Nicolas Martin & Mei Li & Stephen Mann, 2022. "Living material assembly of bacteriogenic protocells," Nature, Nature, vol. 609(7929), pages 1029-1037, September.
    6. Philip Ketterer & Adithya N. Ananth & Diederik S. Laman Trip & Ankur Mishra & Eva Bertosin & Mahipal Ganji & Jaco Torre & Patrick Onck & Hendrik Dietz & Cees Dekker, 2018. "DNA origami scaffold for studying intrinsically disordered proteins of the nuclear pore complex," Nature Communications, Nature, vol. 9(1), pages 1-8, December.
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