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A simple thermodynamic description of phase separation of Nup98 FG domains

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  • Sheung Chun Ng

    (Max Planck Institute for Multidisciplinary Sciences)

  • Dirk Görlich

    (Max Planck Institute for Multidisciplinary Sciences)

Abstract

The permeability barrier of nuclear pore complexes (NPCs) controls nucleocytoplasmic transport. It retains inert macromolecules but allows facilitated passage of nuclear transport receptors that shuttle cargoes into or out of nuclei. The barrier can be described as a condensed phase assembled from cohesive FG repeat domains, including foremost the charge-depleted FG domain of Nup98. We found that Nup98 FG domains show an LCST-type phase separation, and we provide comprehensive and orthogonal experimental datasets for a quantitative description of this behaviour. A derived thermodynamic model correlates saturation concentration with repeat number, temperature, and ionic strength. It allows estimating the enthalpy, entropy, and ΔG (0.2 kJ/mol, 0.1 kB·T) contributions per repeat to phase separation and inter-repeat cohesion. While changing the cohesion strength strongly impacts the strictness of barrier, these numbers provide boundary conditions for in-depth modelling not only of barrier assembly but also of NPC passage.

Suggested Citation

  • Sheung Chun Ng & Dirk Görlich, 2022. "A simple thermodynamic description of phase separation of Nup98 FG domains," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33697-9
    DOI: 10.1038/s41467-022-33697-9
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    References listed on IDEAS

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    1. Franck Delavoie & Vanessa Soldan & Dana Rinaldi & Jean-Yves Dauxois & Pierre-Emmanuel Gleizes, 2019. "The path of pre-ribosomes through the nuclear pore complex revealed by electron tomography," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    2. Jan Andreas Ruland & Annika Marie Krüger & Kerstin Dörner & Rohan Bhatia & Sabine Wirths & Daniel Poetes & Ulrike Kutay & Jan Peter Siebrasse & Ulrich Kubitscheck, 2021. "Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    3. Sheung Chun Ng & Thomas Güttler & Dirk Görlich, 2021. "Recapitulation of selective nuclear import and export with a perfectly repeated 12mer GLFG peptide," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    4. Philip Ketterer & Adithya N. Ananth & Diederik S. Laman Trip & Ankur Mishra & Eva Bertosin & Mahipal Ganji & Jaco Torre & Patrick Onck & Hendrik Dietz & Cees Dekker, 2018. "DNA origami scaffold for studying intrinsically disordered proteins of the nuclear pore complex," Nature Communications, Nature, vol. 9(1), pages 1-8, December.
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    Cited by:

    1. Sheung Chun Ng & Abin Biswas & Trevor Huyton & Jürgen Schünemann & Simone Reber & Dirk Görlich, 2023. "Barrier properties of Nup98 FG phases ruled by FG motif identity and inter-FG spacer length," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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