IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-33697-9.html
   My bibliography  Save this article

A simple thermodynamic description of phase separation of Nup98 FG domains

Author

Listed:
  • Sheung Chun Ng

    (Max Planck Institute for Multidisciplinary Sciences)

  • Dirk Görlich

    (Max Planck Institute for Multidisciplinary Sciences)

Abstract

The permeability barrier of nuclear pore complexes (NPCs) controls nucleocytoplasmic transport. It retains inert macromolecules but allows facilitated passage of nuclear transport receptors that shuttle cargoes into or out of nuclei. The barrier can be described as a condensed phase assembled from cohesive FG repeat domains, including foremost the charge-depleted FG domain of Nup98. We found that Nup98 FG domains show an LCST-type phase separation, and we provide comprehensive and orthogonal experimental datasets for a quantitative description of this behaviour. A derived thermodynamic model correlates saturation concentration with repeat number, temperature, and ionic strength. It allows estimating the enthalpy, entropy, and ΔG (0.2 kJ/mol, 0.1 kB·T) contributions per repeat to phase separation and inter-repeat cohesion. While changing the cohesion strength strongly impacts the strictness of barrier, these numbers provide boundary conditions for in-depth modelling not only of barrier assembly but also of NPC passage.

Suggested Citation

  • Sheung Chun Ng & Dirk Görlich, 2022. "A simple thermodynamic description of phase separation of Nup98 FG domains," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33697-9
    DOI: 10.1038/s41467-022-33697-9
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-33697-9
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-33697-9?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Jan Andreas Ruland & Annika Marie Krüger & Kerstin Dörner & Rohan Bhatia & Sabine Wirths & Daniel Poetes & Ulrike Kutay & Jan Peter Siebrasse & Ulrich Kubitscheck, 2021. "Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    2. Sheung Chun Ng & Thomas Güttler & Dirk Görlich, 2021. "Recapitulation of selective nuclear import and export with a perfectly repeated 12mer GLFG peptide," Nature Communications, Nature, vol. 12(1), pages 1-17, December.
    3. Philip Ketterer & Adithya N. Ananth & Diederik S. Laman Trip & Ankur Mishra & Eva Bertosin & Mahipal Ganji & Jaco Torre & Patrick Onck & Hendrik Dietz & Cees Dekker, 2018. "DNA origami scaffold for studying intrinsically disordered proteins of the nuclear pore complex," Nature Communications, Nature, vol. 9(1), pages 1-8, December.
    4. Franck Delavoie & Vanessa Soldan & Dana Rinaldi & Jean-Yves Dauxois & Pierre-Emmanuel Gleizes, 2019. "The path of pre-ribosomes through the nuclear pore complex revealed by electron tomography," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Sheung Chun Ng & Abin Biswas & Trevor Huyton & Jürgen Schünemann & Simone Reber & Dirk Görlich, 2023. "Barrier properties of Nup98 FG phases ruled by FG motif identity and inter-FG spacer length," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Sheung Chun Ng & Abin Biswas & Trevor Huyton & Jürgen Schünemann & Simone Reber & Dirk Görlich, 2023. "Barrier properties of Nup98 FG phases ruled by FG motif identity and inter-FG spacer length," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    2. Eszter E. Najbauer & Sheung Chun Ng & Christian Griesinger & Dirk Görlich & Loren B. Andreas, 2022. "Atomic resolution dynamics of cohesive interactions in phase-separated Nup98 FG domains," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    3. Martin F. Peter & Jan A. Ruland & Peer Depping & Niels Schneberger & Emmanuele Severi & Jonas Moecking & Karl Gatterdam & Sarah Tindall & Alexandre Durand & Veronika Heinz & Jan Peter Siebrasse & Paul, 2022. "Structural and mechanistic analysis of a tripartite ATP-independent periplasmic TRAP transporter," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    4. Martin F. Peter & Jan A. Ruland & Yeojin Kim & Philipp Hendricks & Niels Schneberger & Jan Peter Siebrasse & Gavin H. Thomas & Ulrich Kubitscheck & Gregor Hagelueken, 2024. "Conformational coupling of the sialic acid TRAP transporter HiSiaQM with its substrate binding protein HiSiaP," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    5. Jan Andreas Ruland & Annika Marie Krüger & Kerstin Dörner & Rohan Bhatia & Sabine Wirths & Daniel Poetes & Ulrike Kutay & Jan Peter Siebrasse & Ulrich Kubitscheck, 2021. "Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
    6. Qi Yang & Xu Chang & Jung Yeon Lee & Minu Saji & Fei Zhang, 2023. "DNA T-shaped crossover tiles for 2D tessellation and nanoring reconfiguration," Nature Communications, Nature, vol. 14(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33697-9. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.