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Characterization of a Salmonella enterica serovar Typhimurium lineage with rough colony morphology and multidrug resistance

Author

Listed:
  • Ying Xiang

    (Center for Disease Control and Prevention of Chinese PLA)

  • Kunpeng Zhu

    (Center for Disease Control and Prevention of Chinese PLA
    Kaifeng Center for Disease Control and Prevention)

  • Kaiyuan Min

    (School of Basic Medicine Peking Union Medical College)

  • Yaowen Zhang

    (Center for Disease Control and Prevention of Chinese PLA
    Daxing Center for Disease Control and Prevention)

  • Jiangfeng Liu

    (School of Basic Medicine Peking Union Medical College)

  • Kangkang Liu

    (Center for Disease Control and Prevention of Chinese PLA)

  • Yiran Han

    (Center for Disease Control and Prevention of Chinese PLA)

  • Xinge Li

    (Center for Disease Control and Prevention of Chinese PLA)

  • Xinying Du

    (Center for Disease Control and Prevention of Chinese PLA)

  • Xin Wang

    (Center for Disease Control and Prevention of Chinese PLA)

  • Ying Huang

    (Center for Disease Control and Prevention of Chinese PLA)

  • Xinping Li

    (Center for Disease Control and Prevention of Chinese PLA)

  • Yuqian Peng

    (Center for Disease Control and Prevention of Chinese PLA)

  • Chaojie Yang

    (Center for Disease Control and Prevention of Chinese PLA)

  • Hongbo Liu

    (Center for Disease Control and Prevention of Chinese PLA)

  • HONGBO Liu

    (Center for Disease Control and Prevention of Chinese PLA)

  • Xiaoying Li

    (Center for Disease Control and Prevention of Chinese PLA)

  • Hui Wang

    (Center for Disease Control and Prevention of Chinese PLA)

  • Chao Wang

    (Center for Disease Control and Prevention of Chinese PLA)

  • Qi Wang

    (Center for Disease Control and Prevention of Chinese PLA)

  • Huiqun Jia

    (Center for Disease Control and Prevention of Chinese PLA)

  • Mingjuan Yang

    (Center for Disease Control and Prevention of Chinese PLA)

  • Ligui Wang

    (Center for Disease Control and Prevention of Chinese PLA)

  • Yarong Wu

    (Beijing Institute of Microbiology and Epidemiology)

  • Yujun Cui

    (Beijing Institute of Microbiology and Epidemiology)

  • Fei Chen

    (China National Center for Bioinformation)

  • Haiyan Yang

    (Zhengzhou University)

  • Stephen Baker

    (Cambridge Biomedical Campus)

  • Xuebin Xu

    (Shanghai Municipal Center for Disease Control and Prevention)

  • Juntao Yang

    (School of Basic Medicine Peking Union Medical College)

  • Hongbin Song

    (Center for Disease Control and Prevention of Chinese PLA)

  • Shaofu Qiu

    (Center for Disease Control and Prevention of Chinese PLA)

Abstract

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a major cause of salmonellosis, and the emergence of multidrug-resistant pathovariants has become a growing concern. Here, we investigate a distinct rough colony variant exhibiting a strong biofilm-forming ability isolated in China. Whole-genome sequencing on 2,212 Chinese isolates and 1,739 publicly available genomes reveals the population structure and evolutionary history of the rough colony variants. Characterized by macro, red, dry, and rough (mrdar) colonies, these variants demonstrate enhanced biofilm formation at 28 °C and 37 °C compared to typical rdar colonies. The mrdar variants exhibit extensive multidrug resistance, with significantly higher resistance to at least five classes of antimicrobial agents compared to non-mrdar variants. This resistance is primarily conferred by an IncHI2 plasmid harboring 19 antimicrobial resistance genes. Phylogenomic analysis divides the global collections into six lineages. The majority of mrdar variants belong to sublineage L6.5, which originated from Chinese smooth colony strains and possibly emerged circa 1977. Among the mrdar variants, upregulation of the csgDEFG operons is observed, probably due to a distinct point mutation (−44G > T) in the csgD gene promoter. Pangenome and genome-wide association analyses identify 87 specific accessory genes and 72 distinct single nucleotide polymorphisms associated with the mrdar morphotype.

Suggested Citation

  • Ying Xiang & Kunpeng Zhu & Kaiyuan Min & Yaowen Zhang & Jiangfeng Liu & Kangkang Liu & Yiran Han & Xinge Li & Xinying Du & Xin Wang & Ying Huang & Xinping Li & Yuqian Peng & Chaojie Yang & Hongbo Liu , 2024. "Characterization of a Salmonella enterica serovar Typhimurium lineage with rough colony morphology and multidrug resistance," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50331-y
    DOI: 10.1038/s41467-024-50331-y
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    References listed on IDEAS

    as
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