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The bacterial replication origin BUS promotes nucleobase capture

Author

Listed:
  • Simone Pelliciari

    (Newcastle University)

  • Salomé Bodet-Lefèvre

    (Queen Mary University of London)

  • Stepan Fenyk

    (Newcastle University)

  • Daniel Stevens

    (Newcastle University)

  • Charles Winterhalter

    (Newcastle University)

  • Frederic D. Schramm

    (Newcastle University)

  • Sara Pintar

    (Newcastle University)

  • Daniel R. Burnham

    (The Francis Crick Institute)

  • George Merces

    (Newcastle University)

  • Tomas T. Richardson

    (Newcastle University)

  • Yumiko Tashiro

    (Queen Mary University of London)

  • Julia Hubbard

    (Newcastle University)

  • Hasan Yardimci

    (The Francis Crick Institute)

  • Aravindan Ilangovan

    (Queen Mary University of London)

  • Heath Murray

    (Newcastle University)

Abstract

Genome duplication is essential for the proliferation of cellular life and this process is generally initiated by dedicated replication proteins at chromosome origins. In bacteria, DNA replication is initiated by the ubiquitous DnaA protein, which assembles into an oligomeric complex at the chromosome origin (oriC) that engages both double-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) to promote DNA duplex opening. However, the mechanism of DnaA specifically opening a replication origin was unknown. Here we show that Bacillus subtilis DnaAATP assembles into a continuous oligomer at the site of DNA melting, extending from a dsDNA anchor to engage a single DNA strand. Within this complex, two nucleobases of each ssDNA binding motif (DnaA-trio) are captured within a dinucleotide binding pocket created by adjacent DnaA proteins. These results provide a molecular basis for DnaA specifically engaging the conserved sequence elements within the bacterial chromosome origin basal unwinding system (BUS).

Suggested Citation

  • Simone Pelliciari & Salomé Bodet-Lefèvre & Stepan Fenyk & Daniel Stevens & Charles Winterhalter & Frederic D. Schramm & Sara Pintar & Daniel R. Burnham & George Merces & Tomas T. Richardson & Yumiko T, 2023. "The bacterial replication origin BUS promotes nucleobase capture," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43823-w
    DOI: 10.1038/s41467-023-43823-w
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    References listed on IDEAS

    as
    1. Karl E. Duderstadt & Kevin Chuang & James M. Berger, 2011. "DNA stretching by bacterial initiators promotes replication origin opening," Nature, Nature, vol. 478(7368), pages 209-213, October.
    2. M. Leaver & P. Domínguez-Cuevas & J. M. Coxhead & R. A. Daniel & J. Errington, 2009. "Life without a wall or division machine in Bacillus subtilis," Nature, Nature, vol. 457(7231), pages 849-853, February.
    3. M. Leaver & P. Domínguez-Cuevas & J. M. Coxhead & R. A. Daniel & J. Errington, 2009. "Erratum: Life without a wall or division machine in Bacillus subtilis," Nature, Nature, vol. 460(7254), pages 538-538, July.
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