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Optogenetic activation of dorsal raphe serotonin neurons induces brain-wide activation

Author

Listed:
  • Hiro Taiyo Hamada

    (Okinawa Institute of Science and Technology Graduate University
    Araya Inc)

  • Yoshifumi Abe

    (Keio University School of Medicine)

  • Norio Takata

    (Keio University School of Medicine)

  • Masakazu Taira

    (Okinawa Institute of Science and Technology Graduate University)

  • Kenji F. Tanaka

    (Keio University School of Medicine)

  • Kenji Doya

    (Okinawa Institute of Science and Technology Graduate University)

Abstract

Serotonin is a neuromodulator that affects multiple behavioral and cognitive functions. Nonetheless, how serotonin causes such a variety of effects via brain-wide projections and various receptors remains unclear. Here we measured brain-wide responses to optogenetic stimulation of serotonin neurons in the dorsal raphe nucleus (DRN) of the male mouse brain using functional MRI with an 11.7 T scanner and a cryoprobe. Transient activation of DRN serotonin neurons caused brain-wide activation, including the medial prefrontal cortex, the striatum, and the ventral tegmental area. The same stimulation under anesthesia with isoflurane decreased brain-wide activation, including the hippocampal complex. These brain-wide response patterns can be explained by DRN serotonergic projection topography and serotonin receptor expression profiles, with enhanced weights on 5-HT1 receptors. Together, these results provide insight into the DR serotonergic system, which is consistent with recent discoveries of its functions in adaptive behaviors.

Suggested Citation

  • Hiro Taiyo Hamada & Yoshifumi Abe & Norio Takata & Masakazu Taira & Kenji F. Tanaka & Kenji Doya, 2024. "Optogenetic activation of dorsal raphe serotonin neurons induces brain-wide activation," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48489-6
    DOI: 10.1038/s41467-024-48489-6
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    References listed on IDEAS

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