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Leucine-rich repeat receptor-like gene screen reveals that Nicotiana RXEG1 regulates glycoside hydrolase 12 MAMP detection

Author

Listed:
  • Yan Wang

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Yuanpeng Xu

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Yujing Sun

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Huibin Wang

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Jiaming Qi

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Bowen Wan

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Wenwu Ye

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Yachun Lin

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Yuanyuan Shao

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Suomeng Dong

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

  • Brett M. Tyler

    (Oregon State University)

  • Yuanchao Wang

    (Nanjing Agricultural University
    Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education))

Abstract

Activation of innate immunity by membrane-localized receptors is conserved across eukaryotes. Plant genomes contain hundreds of such receptor-like genes and those encoding proteins with an extracellular leucine-rich repeat (LRR) domain represent the largest family. Here, we develop a high-throughput approach to study LRR receptor-like genes on a genome-wide scale. In total, 257 tobacco rattle virus-based constructs are generated to target 386 of the 403 identified LRR receptor-like genes in Nicotiana benthamiana for silencing. Using this toolkit, we identify the LRR receptor-like protein Response to XEG1 (RXEG1) that specifically recognizes the glycoside hydrolase 12 protein XEG1. RXEG1 associates with XEG1 via the LRR domain in the apoplast and forms a complex with the LRR receptor-like kinases BAK1 and SOBIR1 to transduce the XEG1-induced defense signal. Thus, this genome-wide silencing assay is demonstrated to be an efficient toolkit to pinpoint new immune receptors, which will contribute to developing durable disease resistance.

Suggested Citation

  • Yan Wang & Yuanpeng Xu & Yujing Sun & Huibin Wang & Jiaming Qi & Bowen Wan & Wenwu Ye & Yachun Lin & Yuanyuan Shao & Suomeng Dong & Brett M. Tyler & Yuanchao Wang, 2018. "Leucine-rich repeat receptor-like gene screen reveals that Nicotiana RXEG1 regulates glycoside hydrolase 12 MAMP detection," Nature Communications, Nature, vol. 9(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-03010-8
    DOI: 10.1038/s41467-018-03010-8
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    Cited by:

    1. Frederickson Entila & Xiaowei Han & Akira Mine & Paul Schulze-Lefert & Kenichi Tsuda, 2024. "Commensal lifestyle regulated by a negative feedback loop between Arabidopsis ROS and the bacterial T2SS," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Li Fan & Katja Fröhlich & Eric Melzer & Rory N. Pruitt & Isabell Albert & Lisha Zhang & Anna Joe & Chenlei Hua & Yanyue Song & Markus Albert & Sang-Tae Kim & Detlef Weigel & Cyril Zipfel & Eunyoung Ch, 2022. "Genotyping-by-sequencing-based identification of Arabidopsis pattern recognition receptor RLP32 recognizing proteobacterial translation initiation factor IF1," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    3. Jinyi Zhu & Qian Qiao & Yujing Sun & Yuanpeng Xu & Haidong Shu & Zhichao Zhang & Fan Liu & Haonan Wang & Wenwu Ye & Suomeng Dong & Yan Wang & Zhenchuan Ma & Yuanchao Wang, 2023. "Divergent sequences of tetraspanins enable plants to specifically recognize microbe-derived extracellular vesicles," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    4. Xiaozhen Zhao & Yiming Wang & Bingqin Yuan & Hanxi Zhao & Yujie Wang & Zheng Tan & Zhiyuan Wang & Huijun Wu & Gang Li & Wei Song & Ravi Gupta & Kenichi Tsuda & Zhonghua Ma & Xuewen Gao & Qin Gu, 2024. "Temporally-coordinated bivalent histone modifications of BCG1 enable fungal invasion and immune evasion," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    5. Wen R. H. Huang & Ciska Braam & Carola Kretschmer & Sergio Landeo Villanueva & Huan Liu & Filiz Ferik & Aranka M. Burgh & Sjef Boeren & Jinbin Wu & Lisha Zhang & Thorsten Nürnberger & Yulu Wang & Mich, 2024. "Receptor-like cytoplasmic kinases of different subfamilies differentially regulate SOBIR1/BAK1-mediated immune responses in Nicotiana benthamiana," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

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