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The EDS1–PAD4–ADR1 node mediates Arabidopsis pattern-triggered immunity

Author

Listed:
  • Rory N. Pruitt

    (University of Tübingen)

  • Federica Locci

    (Max Planck Institute for Plant Breeding Research)

  • Friederike Wanke

    (University of Tübingen)

  • Lisha Zhang

    (University of Tübingen)

  • Svenja C. Saile

    (University of Tübingen)

  • Anna Joe

    (University of Tübingen)

  • Darya Karelina

    (Max Planck Institute for Developmental Biology)

  • Chenlei Hua

    (University of Tübingen)

  • Katja Fröhlich

    (University of Tübingen)

  • Wei-Lin Wan

    (University of Tübingen
    National University of Singapore)

  • Meijuan Hu

    (Chinese Academy of Sciences)

  • Shaofei Rao

    (Chinese Academy of Sciences
    Ningbo University)

  • Sara C. Stolze

    (Max Planck Institute for Plant Breeding Research)

  • Anne Harzen

    (Max Planck Institute for Plant Breeding Research)

  • Andrea A. Gust

    (University of Tübingen)

  • Klaus Harter

    (University of Tübingen)

  • Matthieu H. A. J. Joosten

    (Wageningen University)

  • Bart P. H. J. Thomma

    (Wageningen University
    Cologne University)

  • Jian-Min Zhou

    (Chinese Academy of Sciences)

  • Jeffery L. Dangl

    (University of North Carolina at Chapel Hill)

  • Detlef Weigel

    (Max Planck Institute for Developmental Biology)

  • Hirofumi Nakagami

    (Max Planck Institute for Plant Breeding Research)

  • Claudia Oecking

    (University of Tübingen)

  • Farid El Kasmi

    (University of Tübingen)

  • Jane E. Parker

    (Max Planck Institute for Plant Breeding Research
    Cologne University)

  • Thorsten Nürnberger

    (University of Tübingen
    University of Johannesburg)

Abstract

Plants deploy cell-surface and intracellular leucine rich-repeat domain (LRR) immune receptors to detect pathogens1. LRR receptor kinases and LRR receptor proteins at the plasma membrane recognize microorganism-derived molecules to elicit pattern-triggered immunity (PTI), whereas nucleotide-binding LRR proteins detect microbial effectors inside cells to confer effector-triggered immunity (ETI). Although PTI and ETI are initiated in different host cell compartments, they rely on the transcriptional activation of similar sets of genes2, suggesting pathway convergence upstream of nuclear events. Here we report that PTI triggered by the Arabidopsis LRR receptor protein RLP23 requires signalling-competent dimers of the lipase-like proteins EDS1 and PAD4, and of ADR1 family helper nucleotide-binding LRRs, which are all components of ETI. The cell-surface LRR receptor kinase SOBIR1 links RLP23 with EDS1, PAD4 and ADR1 proteins, suggesting the formation of supramolecular complexes containing PTI receptors and transducers at the inner side of the plasma membrane. We detected similar evolutionary patterns in LRR receptor protein and nucleotide-binding LRR genes across Arabidopsis accessions; overall higher levels of variation in LRR receptor proteins than in LRR receptor kinases are consistent with distinct roles of these two receptor families in plant immunity. We propose that the EDS1–PAD4–ADR1 node is a convergence point for defence signalling cascades, activated by both surface-resident and intracellular LRR receptors, in conferring pathogen immunity.

Suggested Citation

  • Rory N. Pruitt & Federica Locci & Friederike Wanke & Lisha Zhang & Svenja C. Saile & Anna Joe & Darya Karelina & Chenlei Hua & Katja Fröhlich & Wei-Lin Wan & Meijuan Hu & Shaofei Rao & Sara C. Stolze , 2021. "The EDS1–PAD4–ADR1 node mediates Arabidopsis pattern-triggered immunity," Nature, Nature, vol. 598(7881), pages 495-499, October.
  • Handle: RePEc:nat:nature:v:598:y:2021:i:7881:d:10.1038_s41586-021-03829-0
    DOI: 10.1038/s41586-021-03829-0
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    Citations

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    Cited by:

    1. Shen Huang & Chunli Wang & Zixuan Ding & Yaqian Zhao & Jing Dai & Jia Li & Haining Huang & Tongkai Wang & Min Zhu & Mingfeng Feng & Yinghua Ji & Zhongkai Zhang & Xiaorong Tao, 2024. "A plant NLR receptor employs ABA central regulator PP2C-SnRK2 to activate antiviral immunity," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Li Fan & Katja Fröhlich & Eric Melzer & Rory N. Pruitt & Isabell Albert & Lisha Zhang & Anna Joe & Chenlei Hua & Yanyue Song & Markus Albert & Sang-Tae Kim & Detlef Weigel & Cyril Zipfel & Eunyoung Ch, 2022. "Genotyping-by-sequencing-based identification of Arabidopsis pattern recognition receptor RLP32 recognizing proteobacterial translation initiation factor IF1," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    3. Eric Ros-Moner & Tamara Jiménez-Góngora & Luis Villar-Martín & Lana Vogrinec & Víctor M. González-Miguel & Denis Kutnjak & Ignacio Rubio-Somoza, 2024. "Conservation of molecular responses upon viral infection in the non-vascular plant Marchantia polymorpha," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    4. Wen R. H. Huang & Ciska Braam & Carola Kretschmer & Sergio Landeo Villanueva & Huan Liu & Filiz Ferik & Aranka M. Burgh & Sjef Boeren & Jinbin Wu & Lisha Zhang & Thorsten Nürnberger & Yulu Wang & Mich, 2024. "Receptor-like cytoplasmic kinases of different subfamilies differentially regulate SOBIR1/BAK1-mediated immune responses in Nicotiana benthamiana," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    5. Yuankun Yang & Christina E. Steidele & Clemens Rössner & Birgit Löffelhardt & Dagmar Kolb & Thomas Leisen & Weiguo Zhang & Christina Ludwig & Georg Felix & Michael F. Seidl & Annette Becker & Thorsten, 2023. "Convergent evolution of plant pattern recognition receptors sensing cysteine-rich patterns from three microbial kingdoms," Nature Communications, Nature, vol. 14(1), pages 1-12, December.

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