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bacLIFE: a user-friendly computational workflow for genome analysis and prediction of lifestyle-associated genes in bacteria

Author

Listed:
  • Guillermo Guerrero-Egido

    (Leiden University
    Netherlands Institute of Ecology (NIOO-KNAW)
    Campus Universitario de Teatinos s/n, Universidad de Málaga
    Campus Universitario de Teatinos, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC))

  • Adrian Pintado

    (Leiden University
    Campus Universitario de Teatinos s/n, Universidad de Málaga
    Campus Universitario de Teatinos, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC))

  • Kevin M. Bretscher

    (Leiden University
    Netherlands Institute of Ecology (NIOO-KNAW)
    Campus Universitario de Teatinos s/n, Universidad de Málaga
    Campus Universitario de Teatinos, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC))

  • Luisa-Maria Arias-Giraldo

    (Netherlands Institute of Ecology (NIOO-KNAW))

  • Joseph N. Paulson

    (N-Power Medicine)

  • Herman P. Spaink

    (Leiden University)

  • Dennis Claessen

    (Leiden University)

  • Cayo Ramos

    (Campus Universitario de Teatinos, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC)
    Campus Universitario de Teatinos s/n, Universidad de Málaga)

  • Francisco M. Cazorla

    (Campus Universitario de Teatinos s/n, Universidad de Málaga
    Campus Universitario de Teatinos, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC))

  • Marnix H. Medema

    (Leiden University
    Wageningen University)

  • Jos M. Raaijmakers

    (Leiden University
    Netherlands Institute of Ecology (NIOO-KNAW))

  • Víctor J. Carrión

    (Leiden University
    Netherlands Institute of Ecology (NIOO-KNAW)
    Campus Universitario de Teatinos s/n, Universidad de Málaga
    Campus Universitario de Teatinos, Universidad de Málaga-Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC))

Abstract

Bacteria have an extensive adaptive ability to live in close association with eukaryotic hosts, exhibiting detrimental, neutral or beneficial effects on host growth and health. However, the genes involved in niche adaptation are mostly unknown and their functions poorly characterized. Here, we present bacLIFE ( https://github.com/Carrion-lab/bacLIFE ) a streamlined computational workflow for genome annotation, large-scale comparative genomics, and prediction of lifestyle-associated genes (LAGs). As a proof of concept, we analyzed 16,846 genomes from the Burkholderia/Paraburkholderia and Pseudomonas genera, which led to the identification of hundreds of genes potentially associated with a plant pathogenic lifestyle. Site-directed mutagenesis of 14 of these predicted LAGs of unknown function, followed by plant bioassays, showed that 6 predicted LAGs are indeed involved in the phytopathogenic lifestyle of Burkholderia plantarii and Pseudomonas syringae pv. phaseolicola. These 6 LAGs encompassed a glycosyltransferase, extracellular binding proteins, homoserine dehydrogenases and hypothetical proteins. Collectively, our results highlight bacLIFE as an effective computational tool for prediction of LAGs and the generation of hypotheses for a better understanding of bacteria-host interactions.

Suggested Citation

  • Guillermo Guerrero-Egido & Adrian Pintado & Kevin M. Bretscher & Luisa-Maria Arias-Giraldo & Joseph N. Paulson & Herman P. Spaink & Dennis Claessen & Cayo Ramos & Francisco M. Cazorla & Marnix H. Mede, 2024. "bacLIFE: a user-friendly computational workflow for genome analysis and prediction of lifestyle-associated genes in bacteria," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46302-y
    DOI: 10.1038/s41467-024-46302-y
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    References listed on IDEAS

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