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Gene drive and genetic sex conversion in the global agricultural pest Ceratitis capitata

Author

Listed:
  • Angela Meccariello

    (Imperial College London)

  • Shibo Hou

    (Imperial College London)

  • Serafima Davydova

    (Imperial College London)

  • James Daniel Fawcett

    (Imperial College London)

  • Alexandra Siddall

    (University of East Anglia, Norwich Research Park)

  • Philip T. Leftwich

    (University of East Anglia, Norwich Research Park)

  • Flavia Krsticevic

    (Hebrew University of Jerusalem)

  • Philippos Aris Papathanos

    (Hebrew University of Jerusalem)

  • Nikolai Windbichler

    (Imperial College London)

Abstract

Homing-based gene drives are recently proposed interventions promising the area-wide, species-specific genetic control of harmful insect populations. Here we characterise a first set of gene drives in a tephritid agricultural pest species, the Mediterranean fruit fly Ceratitis capitata (medfly). Our results show that the medfly is highly amenable to homing-based gene drive strategies. By targeting the medfly transformer gene, we also demonstrate how CRISPR-Cas9 gene drive can be coupled to sex conversion, whereby genetic females are transformed into fertile and harmless XX males. Given this unique malleability of sex determination, we modelled gene drive interventions that couple sex conversion and female sterility and found that such approaches could be effective and tolerant of resistant allele selection in the target population. Our results open the door for developing gene drive strains for the population suppression of the medfly and related tephritid pests by co-targeting female reproduction and shifting the reproductive sex ratio towards males. They demonstrate the untapped potential for gene drives to tackle agricultural pests in an environmentally friendly and economical way.

Suggested Citation

  • Angela Meccariello & Shibo Hou & Serafima Davydova & James Daniel Fawcett & Alexandra Siddall & Philip T. Leftwich & Flavia Krsticevic & Philippos Aris Papathanos & Nikolai Windbichler, 2024. "Gene drive and genetic sex conversion in the global agricultural pest Ceratitis capitata," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-023-44399-1
    DOI: 10.1038/s41467-023-44399-1
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    References listed on IDEAS

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    1. Hannah A. Grunwald & Valentino M. Gantz & Gunnar Poplawski & Xiang-Ru S. Xu & Ethan Bier & Kimberly L. Cooper, 2019. "Super-Mendelian inheritance mediated by CRISPR–Cas9 in the female mouse germline," Nature, Nature, vol. 566(7742), pages 105-109, February.
    2. Nikolay P. Kandul & Junru Liu & Hector M. Sanchez C. & Sean L. Wu & John M. Marshall & Omar S. Akbari, 2019. "Transforming insect population control with precision guided sterile males with demonstration in flies," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    3. Roberto Galizi & Lindsey A. Doyle & Miriam Menichelli & Federica Bernardini & Anne Deredec & Austin Burt & Barry L. Stoddard & Nikolai Windbichler & Andrea Crisanti, 2014. "A synthetic sex ratio distortion system for the control of the human malaria mosquito," Nature Communications, Nature, vol. 5(1), pages 1-8, September.
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    Cited by:

    1. Michelle L. Johnson & Bruce A. Hay & Maciej Maselko, 2024. "Altering traits and fates of wild populations with Mendelian DNA sequence modifying Allele Sails," Nature Communications, Nature, vol. 15(1), pages 1-12, December.

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