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CRISPR-based gene drives generate super-Mendelian inheritance in the disease vector Culex quinquefasciatus

Author

Listed:
  • Tim Harvey-Samuel

    (The Pirbright Institute)

  • Xuechun Feng

    (University of California San Diego
    Shenzhen Bay Laboratory)

  • Emily M. Okamoto

    (University of California San Diego)

  • Deepak-Kumar Purusothaman

    (The Pirbright Institute
    University of Glasgow)

  • Philip T. Leftwich

    (University of East Anglia, Norwich Research Park)

  • Luke Alphey

    (The Pirbright Institute
    University of York)

  • Valentino M. Gantz

    (University of California San Diego)

Abstract

Culex mosquitoes pose a significant public health threat as vectors for a variety of diseases including West Nile virus and lymphatic filariasis, and transmit pathogens threatening livestock, companion animals, and endangered birds. Rampant insecticide resistance makes controlling these mosquitoes challenging and necessitates the development of new control strategies. Gene drive technologies have made significant progress in other mosquito species, although similar advances have been lagging in Culex. Here we test a CRISPR-based homing gene drive for Culex quinquefasciatus, and show that the inheritance of two split-gene-drive transgenes, targeting different loci, are biased in the presence of a Cas9-expressing transgene although with modest efficiencies. Our findings extend the list of disease vectors where engineered homing gene drives have been demonstrated to include Culex alongside Anopheles and Aedes, and pave the way for future development of these technologies to control Culex mosquitoes.

Suggested Citation

  • Tim Harvey-Samuel & Xuechun Feng & Emily M. Okamoto & Deepak-Kumar Purusothaman & Philip T. Leftwich & Luke Alphey & Valentino M. Gantz, 2023. "CRISPR-based gene drives generate super-Mendelian inheritance in the disease vector Culex quinquefasciatus," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41834-1
    DOI: 10.1038/s41467-023-41834-1
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    References listed on IDEAS

    as
    1. Jackson Champer & Esther Lee & Emily Yang & Chen Liu & Andrew G. Clark & Philipp W. Messer, 2020. "A toxin-antidote CRISPR gene drive system for regional population modification," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    2. Nikolay P. Kandul & Junru Liu & Hector M. Sanchez C. & Sean L. Wu & John M. Marshall & Omar S. Akbari, 2019. "Transforming insect population control with precision guided sterile males with demonstration in flies," Nature Communications, Nature, vol. 10(1), pages 1-12, December.
    3. Xuechun Feng & Víctor López Del Amo & Enzo Mameli & Megan Lee & Alena L. Bishop & Norbert Perrimon & Valentino M. Gantz, 2021. "Optimized CRISPR tools and site-directed transgenesis towards gene drive development in Culex quinquefasciatus mosquitoes," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
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