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Dynamical control enables the formation of demixed biomolecular condensates

Author

Listed:
  • Andrew Z. Lin

    (Washington University in St. Louis)

  • Kiersten M. Ruff

    (Washington University in St. Louis)

  • Furqan Dar

    (Washington University in St. Louis)

  • Ameya Jalihal

    (Duke University)

  • Matthew R. King

    (Washington University in St. Louis)

  • Jared M. Lalmansingh

    (Washington University in St. Louis)

  • Ammon E. Posey

    (Washington University in St. Louis)

  • Nadia A. Erkamp

    (Washington University in St. Louis
    University of Cambridge, Lensfield Road)

  • Ian Seim

    (Duke University)

  • Amy S. Gladfelter

    (Duke University)

  • Rohit V. Pappu

    (Washington University in St. Louis)

Abstract

Cellular matter can be organized into compositionally distinct biomolecular condensates. For example, in Ashbya gossypii, the RNA-binding protein Whi3 forms distinct condensates with different RNA molecules. Using criteria derived from a physical framework for explaining how compositionally distinct condensates can form spontaneously via thermodynamic considerations, we find that condensates in vitro form mainly via heterotypic interactions in binary mixtures of Whi3 and RNA. However, within these condensates, RNA molecules become dynamically arrested. As a result, in ternary systems, simultaneous additions of Whi3 and pairs of distinct RNA molecules lead to well-mixed condensates, whereas delayed addition of an RNA component results in compositional distinctness. Therefore, compositional identities of condensates can be achieved via dynamical control, being driven, at least partially, by the dynamical arrest of RNA molecules. Finally, we show that synchronizing the production of different RNAs leads to more well-mixed, as opposed to compositionally distinct condensates in vivo.

Suggested Citation

  • Andrew Z. Lin & Kiersten M. Ruff & Furqan Dar & Ameya Jalihal & Matthew R. King & Jared M. Lalmansingh & Ammon E. Posey & Nadia A. Erkamp & Ian Seim & Amy S. Gladfelter & Rohit V. Pappu, 2023. "Dynamical control enables the formation of demixed biomolecular condensates," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43489-4
    DOI: 10.1038/s41467-023-43489-4
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    References listed on IDEAS

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    2. Aniruddha Chattaraj & Eugene I. Shakhnovich, 2024. "Multi-condensate state as a functional strategy to optimize the cell signaling output," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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