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Intrinsically disordered CsoS2 acts as a general molecular thread for α-carboxysome shell assembly

Author

Listed:
  • Tao Ni

    (University of Oxford
    The University of Hong Kong, Pokfulam)

  • Qiuyao Jiang

    (University of Liverpool)

  • Pei Cing Ng

    (University of Liverpool)

  • Juan Shen

    (University of Oxford)

  • Hao Dou

    (University of Oxford)

  • Yanan Zhu

    (University of Oxford)

  • Julika Radecke

    (Diamond Light Source, Harwell Science and Innovation Campus)

  • Gregory F. Dykes

    (University of Liverpool)

  • Fang Huang

    (University of Liverpool)

  • Lu-Ning Liu

    (University of Liverpool
    Ocean University of China)

  • Peijun Zhang

    (University of Oxford
    Diamond Light Source, Harwell Science and Innovation Campus
    University of Oxford)

Abstract

Carboxysomes are a paradigm of self-assembling proteinaceous organelles found in nature, offering compartmentalisation of enzymes and pathways to enhance carbon fixation. In α-carboxysomes, the disordered linker protein CsoS2 plays an essential role in carboxysome assembly and Rubisco encapsulation. Its mechanism of action, however, is not fully understood. Here we synthetically engineer α-carboxysome shells using minimal shell components and determine cryoEM structures of these to decipher the principle of shell assembly and encapsulation. The structures reveal that the intrinsically disordered CsoS2 C-terminus is well-structured and acts as a universal “molecular thread” stitching through multiple shell protein interfaces. We further uncover in CsoS2 a highly conserved repetitive key interaction motif, [IV]TG, which is critical to the shell assembly and architecture. Our study provides a general mechanism for the CsoS2-governed carboxysome shell assembly and cargo encapsulation and further advances synthetic engineering of carboxysomes for diverse biotechnological applications.

Suggested Citation

  • Tao Ni & Qiuyao Jiang & Pei Cing Ng & Juan Shen & Hao Dou & Yanan Zhu & Julika Radecke & Gregory F. Dykes & Fang Huang & Lu-Ning Liu & Peijun Zhang, 2023. "Intrinsically disordered CsoS2 acts as a general molecular thread for α-carboxysome shell assembly," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41211-y
    DOI: 10.1038/s41467-023-41211-y
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    References listed on IDEAS

    as
    1. Lauren Ann Metskas & Davi Ortega & Luke M. Oltrogge & Cecilia Blikstad & Derik R. Lovejoy & Thomas G. Laughlin & David F. Savage & Grant J. Jensen, 2022. "Rubisco forms a lattice inside alpha-carboxysomes," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
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