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Spatial transcriptomics analysis of esophageal squamous precancerous lesions and their progression to esophageal cancer

Author

Listed:
  • Xuejiao Liu

    (Zhengzhou University
    China-US (Henan) Hormel Cancer Institute)

  • Simin Zhao

    (China-US (Henan) Hormel Cancer Institute
    Affiliated Cancer Hospital of Zhengzhou University
    Zhengzhou University)

  • Keke Wang

    (China-US (Henan) Hormel Cancer Institute)

  • Liting Zhou

    (Zhengzhou University
    China-US (Henan) Hormel Cancer Institute
    Zhengzhou University)

  • Ming Jiang

    (China-US (Henan) Hormel Cancer Institute)

  • Yunfeng Gao

    (China-US (Henan) Hormel Cancer Institute)

  • Ran Yang

    (China-US (Henan) Hormel Cancer Institute)

  • Shiwen Yan

    (Zhengzhou University
    China-US (Henan) Hormel Cancer Institute
    Zhengzhou University)

  • Wen Zhang

    (Zhengzhou University
    China-US (Henan) Hormel Cancer Institute
    Zhengzhou University)

  • Bingbing Lu

    (Zhengzhou University
    China-US (Henan) Hormel Cancer Institute
    Zhengzhou University)

  • Feifei Liu

    (Zhengzhou University
    China-US (Henan) Hormel Cancer Institute
    Zhengzhou University)

  • Ran Zhao

    (Zhengzhou University
    China-US (Henan) Hormel Cancer Institute)

  • Wenting Liu

    (Zhengzhou University
    China-US (Henan) Hormel Cancer Institute)

  • Zihan Zhang

    (Zhengzhou University
    China-US (Henan) Hormel Cancer Institute
    Zhengzhou University)

  • Kangdong Liu

    (Zhengzhou University
    China-US (Henan) Hormel Cancer Institute
    Zhengzhou University
    The Collaborative Innovation Center of Henan Province for Cancer Chemoprevention)

  • Xiang Li

    (Zhengzhou University
    China-US (Henan) Hormel Cancer Institute
    Zhengzhou University
    The Collaborative Innovation Center of Henan Province for Cancer Chemoprevention)

  • Zigang Dong

    (Zhengzhou University
    China-US (Henan) Hormel Cancer Institute
    Zhengzhou University
    The Collaborative Innovation Center of Henan Province for Cancer Chemoprevention)

Abstract

Esophageal squamous precancerous lesions (ESPL) are the precursors of esophageal squamous cell carcinoma (ESCC) including low-grade and high-grade intraepithelial neoplasia. Due to the absence of molecular indicators, which ESPL will eventually develop into ESCC and thus should be treated is not well defined. Indicators, for predicting risks of ESCC at ESPL stages, are an urgent need. We perform spatial whole-transcriptome atlas analysis, which can eliminate other tissue interference by sequencing the specific ESPL regions. In this study, the expression of TAGLN2 significantly increases, while CRNN expression level decreases along the progression of ESCC. Additionally, TAGLN2 protein level significantly increases in paired after-progression tissues compared with before-progression samples, while CRNN expression decreases. Functional studies suggest that TAGLN2 promotes ESCC progression, while CRNN inhibits it by regulating cell proliferation. Taken together, TAGLN2 and CRNN are suggested as candidate indicators for the risk of ESCC at ESPL stages.

Suggested Citation

  • Xuejiao Liu & Simin Zhao & Keke Wang & Liting Zhou & Ming Jiang & Yunfeng Gao & Ran Yang & Shiwen Yan & Wen Zhang & Bingbing Lu & Feifei Liu & Ran Zhao & Wenting Liu & Zihan Zhang & Kangdong Liu & Xia, 2023. "Spatial transcriptomics analysis of esophageal squamous precancerous lesions and their progression to esophageal cancer," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40343-5
    DOI: 10.1038/s41467-023-40343-5
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