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Single cell transcriptomic analysis reveals cellular diversity of murine esophageal epithelium

Author

Listed:
  • Mohammad Faujul Kabir

    (Temple University Lewis Katz School of Medicine)

  • Adam L. Karami

    (Temple University Lewis Katz School of Medicine)

  • Ricardo Cruz-Acuña

    (Columbia University Medical Center)

  • Alena Klochkova

    (Temple University Lewis Katz School of Medicine)

  • Reshu Saxena

    (Temple University Lewis Katz School of Medicine)

  • Anbin Mu

    (Temple University Lewis Katz School of Medicine)

  • Mary Grace Murray

    (Temple University Lewis Katz School of Medicine)

  • Jasmine Cruz

    (Temple University Lewis Katz School of Medicine)

  • Annie D. Fuller

    (Temple University Lewis Katz School of Medicine)

  • Margarette H. Clevenger

    (Northwestern University Feinberg School of Medicine)

  • Kumaraswamy Naidu Chitrala

    (Temple University Lewis Katz School of Medicine)

  • Yinfei Tan

    (Fox Chase Cancer Center)

  • Kelsey Keith

    (Coriell Institute for Medical Research)

  • Jozef Madzo

    (Coriell Institute for Medical Research)

  • Hugh Huang

    (Coriell Institute for Medical Research)

  • Jaroslav Jelinek

    (Coriell Institute for Medical Research)

  • Tatiana Karakasheva

    (Children’s Hospital of Philadelphia)

  • Kathryn E. Hamilton

    (Children’s Hospital of Philadelphia)

  • Amanda B. Muir

    (Children’s Hospital of Philadelphia)

  • Marie-Pier Tétreault

    (Northwestern University Feinberg School of Medicine)

  • Kelly A. Whelan

    (Temple University Lewis Katz School of Medicine
    Temple University Lewis Katz School of Medicine)

Abstract

Although morphologic progression coupled with expression of specific molecular markers has been characterized along the esophageal squamous differentiation gradient, the molecular heterogeneity within cell types along this trajectory has yet to be classified at the single cell level. To address this knowledge gap, we perform single cell RNA-sequencing of 44,679 murine esophageal epithelial, to identify 11 distinct cell populations as well as pathways alterations along the basal-superficial axis and in each individual population. We evaluate the impact of aging upon esophageal epithelial cell populations and demonstrate age-associated mitochondrial dysfunction. We compare single cell transcriptomic profiles in 3D murine organoids and human esophageal biopsies with that of murine esophageal epithelium. Finally, we employ pseudotemporal trajectory analysis to develop a working model of cell fate determination in murine esophageal epithelium. These studies provide comprehensive molecular perspective on the cellular heterogeneity of murine esophageal epithelium in the context of homeostasis and aging.

Suggested Citation

  • Mohammad Faujul Kabir & Adam L. Karami & Ricardo Cruz-Acuña & Alena Klochkova & Reshu Saxena & Anbin Mu & Mary Grace Murray & Jasmine Cruz & Annie D. Fuller & Margarette H. Clevenger & Kumaraswamy Nai, 2022. "Single cell transcriptomic analysis reveals cellular diversity of murine esophageal epithelium," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-29747-x
    DOI: 10.1038/s41467-022-29747-x
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    References listed on IDEAS

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    Cited by:

    1. Lana Kostic & Carly Leung & Katzrin Ahmad Murad & Snezhina Kancheva & Stefano Perna & Bernett Lee & Nick Barker, 2024. "Lgr5 marks stem/progenitor cells contributing to epithelial and muscle development in the mouse esophagus," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    2. Naveen Kumar & Pon Ganish Prakash & Christian Wentland & Shilpa Mary Kurian & Gaurav Jethva & Volker Brinkmann & Hans-Joachim Mollenkopf & Tobias Krammer & Christophe Toussaint & Antoine-Emmanuel Sali, 2024. "Decoding spatiotemporal transcriptional dynamics and epithelial fibroblast crosstalk during gastroesophageal junction development through single cell analysis," Nature Communications, Nature, vol. 15(1), pages 1-20, December.

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